Chloridium gonytrichii (F.A. Fernández & Huhndorf) Réblová & Seifert, IMA Fungus

Wei, Tian-Peng, Wang, Kun-Ying, Zhang, Hong, Luo, Ming-Yan, Jia, Wei-Yu, Zeng, Yan & Jiang, Yu-Lan, 2022, A new species and a new combination of Chloridium from southwest China, Phytotaxa 549 (1), pp. 67-76 : 73

Chloridium gonytrichii (F.A. Fernández & Huhndorf) Réblová & Seifert, IMA Fungus 7 (1): 134 (2016) ( Fig. 4 )

Description:— Mycelium immersed or superficial, composed of hyaline to light brown, smooth, septate, 2–4 μm wide hyphae. Conidiophores solitary, tapering from the swollen base towards the apex, unbranched, septate, dark brown and paler towards the apex, some with a terminal sporulating phialide, others with a sterile ending, with 2–5 whorls of phialides in the midsection to lower section, 165–356 × 4.5–7.0 μm. Conidiogenous cells cylindrical to lageniform, producing conidia from multiple enteroblastic conidiogenous loci, phialides borne on collar hyphae around the conidiophore, 7.5–18.5 × 3.5–5.0 µm. Conidia ellipsoidal to subglobose, aseptate, colorless or olivaceous to light green, smooth, guttulate, 3.0–5.0 × 2.5–3.0 μm. Sexual stage not observed.

Culture characteristics:—Colonies on PDA flattened, with rough surface and dense mycelia, light olivaceous to dark olivaceous brown, with a broad hyaline margin, reaching 24 mm diam at 25 ± 1 °C after 7 days.

Material examined:— CHINA, Guizhou Province: Kaili City , isolated from forest soil, 26.2402°N, 107.7722°E, 1500 m above sea level, 15 March 2018, K.Y. Wang & T . P. Wei ( HGUP 1805 ), living culture CGMCC3.19590 and GUCC 1805 .

Notes:—Multi-locus phylogenetic analyses indicated that our isolate CGMCC3.19590 clusters in a clade closely related to C. gonytrichii with high statistical support ( Fig. 1 ). Morphologically, the conidial dimensions in the present study fit exactly with those in Bao et al. (2021). Therefore, this species was identified as C. gonytrichii by combined morphology and phylogenetic analyses. Moreover, this report of this poorly known taxon extends its distribution to southwest China from its original location in Puerto Rico ( Réblová et al. 2016).

Figures

FIGURE 1. Phylogram of RAxML analysis based on a combined ITS and LSU sequence dataset within Chloridium. Adautomilanezia caesalpiniae was used as outgroup. Bootstrap support values for Maximum likelihood and Maximum parsimony greater than 50%, and Bayesian posterior probabilities equal to or greater than 0.8 are indicated at the nodes (ML/BI/MP). Type strains are highlighted by a “*”, and our strain is in bold.

FIGURE 4. Chloridium gonytrichii (CGMCC3.19590).A–B: Colony on PDA for 2 weeks. C–D: Conidiophores, conidiogenous cells and conidia. E–F: Conidiogenous cells producing conidia. G: Conidia. Scale bars: C–D = 100 μm; E–F = 10 μm; G = 5 μm.