Torula suae W.P. Wang, H.W. Shen & Z.L. Luo, 2023

Wang, Wen-Peng, Shen, Hong-Wei, Bao, Dan-Feng, Lu, Yong-Zhong, Yang, Qiu-Xia, Su, Xi-Jun & Luo, Zong-Long, 2023, Two novel species and three new records of Torulaceae from Yunnan Province, China, MycoKeys 99, pp. 1-24 : 1

Torula suae W.P. Wang, H.W. Shen & Z.L. Luo sp. nov.

Fig. 5

Etymology.

“suae” (Lat) in memory of the Chinese mycologist Prof. Hong-Yan Su, who kindly helped the authors in many ways and sadly passed away on 3 May 2022.

Holotype.

KUN-HKAS 124620.

Description.

Saprobic on submerged decaying wood. Sexual morph: Undetermined. Asexual morph: Colonies effuse on the natural substrate, neat, hairy, brown to dark brown. Mycelium immersed to superficial, composed of hyaline, becoming brown closer to fertile region, septate, branched hyphae. Conidiophores 17-54 × 3-4 μm (x̄ = 32 × 3 μm, n = 10), macronematous to semi- macronematous, erect, straight, or slightly flexuous, without apical branches, light brown to brown, ellipsoid to subcylindrical, smooth, septate. Conidiogenous cells 6-8 × 5-7 μm (x̄ = 7 × 6 μm, n = 20), mono- to polyblastic, integrated, terminal or intercalary, doliiform to subglobose, brown to dark brown. Conidia (16-) 31-115 (-160) × 6-9 μm (x̄ = 69 × 7 μm, n = 35), in branched chains, acrogenous, phragmoconidia, golden at apex, brown to dark brown, 2-29-septate, constricted at the septa, verrucose, easily separating, guttulate, chiefly subcylindrical, globlose to subglobose of each cell.

Culture characteristics.

Conidia germinating on PDA within 12 h, and germ tubes produced at the side. Mycelium superficial, branched, septate, hyaline, smooth. After two weeks of incubation at room temperature, colony appears distinctly rounded, the central hyphae are longer, white, velvety, and the edges are brown and the hyphae are shorter.

Material examined.

China, Yunnan Province, Wenshan City, Bamei Town (24°31′96.49″N, 105°03′84.35″E), on submerged decaying wood, 7 February 2022, Wen-Peng Wang S-3509 (KUN-HKAS 124620, holotype), ex-type living culture, KUNCC 22-12433 = CGMCC 3.24259 .

Notes.

According to the BLASTn results, the closest matches for our new species were Torula suae (KUNCC 22-12433) (NR 159045, 98.59% similarity in ITS) and T. goaensis (NFCCL 4040) (NG 060016, 99.60% similarity in LSU). Comparison of ITS and LSU nucleotide bases indicated that T. suae differs from T. goaensis in 7/494 (ITS) and 5/1257 (LSU). Phylogenetic analyses showed that T. suae clustered with T. goaensis with low support. Morphologically, T. suae is similar to T. goaensis in having conidiophores without apical branches and doliiform to subglobose conidiogenous cells ( Pratibha and Prabhugaonkar 2017). However, T. suae can be distinguished from T. goaensis by the conidial type; T. goaensis has phragmoconidia and scolecoconidia, whereas T. suae only produces phragmoconidia, and T. suae has more conidial septa (2-29 vs. 7-20).

Figures

Figure 5. Torula suae (KUN-HKAS 124620, holotype) A fungal structure on the substratum C, D Conidiophores with conidiogenous cells B, E-I conidia J germinating conidium K, L colonies on PDA from surface and reverse. Scale bars: 60 μm (B), 10 μm (C-F), 20 μm (G, H), 30 μm (I, J).