Typhlodromalus annulatus, Carvalho, Adeilma Nascimento De, Oliveira, Aníbal Ramadan, Argolo, Poliane Sá & Ferragut, Francisco, 2017

Carvalho, Adeilma Nascimento De, Oliveira, Aníbal Ramadan, Argolo, Poliane Sá & Ferragut, Francisco, 2017, Three new species of phytoseiid mites (Acari: Phytoseiidae) from Bahia State, Brazil, Zootaxa 4272 (1), pp. 142-150 : 147-149

Typhlodromalus annulatus n. sp. Ferragut & Carvalho

Diagnosis. Female dorsal shield mostly areolate. Dorsal setal pattern 10A:9B. Seventeen pairs of stout dorsal setae, all smooth except Z4, Z5 and J5 serrated. Most setae stout and blunt and arising from tubercles. Seta Z1 short, less than 15 long. Ratio s4 / Z1> 7. Seven pairs of dorsal solenostomes. Peritremes extending to j1. Sternal shield with three pairs of setae, posterior margin indiscernible. Ventrianal shield smooth and vase-shaped, with anterior margin straight and lateral margins concave. Three pairs of pre-anal setae; pre-anal pores crescentic. Fixed digit with nine teeth, movable digit with three teeth. Major duct broad and membranous, surrounded by an incomplete sclerotised ring; atrium globular and tulip-like, incised laterally; calyx tubular flaring distally. Genu II with eight setae. Three stout and blunt macrosetae on leg IV.

FEMALE ( Figs 13–17 ) (two females measured).

Dorsal surface. Dorsal shield 356 (354–356) long, 213 (213–225) wide, covered by areolate elements between setae j1 and Z4; anterolateral margins striate; smooth surface behind Z4. Seventeen pairs of smooth setae, except Z4, Z5 and J5, which are slightly serrated. All setae thick and strong, j4, j5, j6, J5, Z1 and z5 acute, the remainder blunt. Seta j1 32 (30–32), j3 46 (46–50), j4 8 (8–9), j5 8 (8–9), j6 9, J2 14 (14–15), J5 8, z2 24, z4 56 (56–58), z5 6, Z1 13 (13–15), Z4 65 (65–69), Z5 88 (84–88), s4 93, S2 45 (45–48), S4 33 (33–36), S5 15 (15–16), r3 32 (32–36), R1 16 (16–17). Seven pairs of solenostomes; gd1, gd2, gd4 and gd5 small and punctiform; gd6, gd8, gd9 small and crateriform. Solenostome gd2 close to z4, gd8 anterolaterad to Z4, gd9 anteromediad to S5. Peritremes extending to setae j1.

Ventral surface. Sternal shield smooth and weakly sclerotised, with three pairs of setae, posterior margin indiscernible, somewhat wider than long, distance st2–st2 79, distance st1–st3 70. Seta st4 on striated integument. Genital shield 86 wide. Ventrianal shield vase-shaped, smooth and poorly sclerotised, with anterior margin straight and lateral margins concave, longer than wide, 123 long, 81 wide at level of ZV2, 69 wide at level of anus. Three pairs of pre-anal setae; pre-anal (gv3) pores crescentic, distance between them 34. Four pairs of setae surrounding the shield, JV5 43 (43–48). One pair of large pores associated with JV5.

Chelicerae. Fixed digit 31 (27–31) long, movable digit 28 (28–30) long. Fixed digit with nine teeth, two subapical denticles and seven larger and teeth in comb-like alignment. Movable digit with three well-separated teeth.

Insemination apparatus. Complex, major duct broad and membranous, an incomplete sclerotised ring encircling the duct; atrium globular and tulip-like, incised laterally; calyx tubular flaring distally, 15 long.

Legs. Genu II with eight setae; 2 2/1 2/0 1. Macrosetae present on all legs, on genu I 35 (31–35), genu II 31 (27–31), genu III 36 (33–36), tibia III 22 (21–22), tarsus III 18; on leg IV, genu 61 (57–61), tibia 33 (28–33) and basitarsus 83 (78–83). All macrosetae broad and blunt, some of the remainder setae in the segments are acute, while others are blunt.

MALE: ( Figs 18–19 ) (one male measured)

Dorsal surface. Dorsal shield pattern as in female, 264 long, 174 wide. Seventeen pairs of smooth setae, except Z4, Z5 and J5, which are slightly serrated. All setae thick and strong, j4, j5, j6, J5, Z1 and z5 acute, the remainder blunt. Seta j1 24, j3 39, j4 6, j5 6, j6 8, J2 9, J5 6, z2 16, z4 42, z5 4, Z1 9, Z4 39, Z5 48, s4 60, S2 18, S4 15, S5 9, r3 27, R1 11. Seven pairs of solenostomes; gd1, gd2, gd4 and gd5 small and punctiform; gd6, gd8, gd9 small and crateriform. Solenostome gd2 close to z4, gd8 anterolaterad to Z4, gd9 anteromediad to S5. Peritremes extending to setae j1.

Ventral surface. Ventrianal shield subtriangular, reticulate mainly anteriorly to pre-anal setae; 105 long and 141 wide at anterior corners. Three pairs of pre-anal setae; pre-anal (gv3) pores crescentic, distance between them 24. Seta JV5 20.

Chelicerae. Fixed and movable digits 36 long. Fixed digit with seven teeth. Movable digit with one tooth. Spermatodactyl 18 long.

Legs. Genu II with eight setae; 2 2/1 2/0 1. Macrosetae on all legs, on genu I 27, genu II 18, genu III 23, tibia III 21, tarsus III 18; on leg IV, genu 36, tibia 24 and basitarsus 63. All macrosetae broad and blunt, some of the remaining setae in the segments are acute, while others are blunt.

Type Material: Holotype female, one paratype female and one paratype male from Inga affinis Benth (Fabaceae) , Arataca , Bahia ( Brazil) 14°47'50" S, 39°10'14" W, 20 January 2015, 26 October 2016, 92 m. a.s.l . Holotype and paratypes deposited in the collection of Acari at Laboratory of Entomology, Universidade Estadual de Santa Cruz, Ilhéus - BA, Brazil .

Etymology: The specific name annulatus derives from the Latin annulus, which means ring and refers to the incomplete ring encircling the major duct in the spermatheca.

Remarks: Chant & McMurtry (2005) proposed the genus Amblydromalus , which was distinguished from Typhlodromalus Muma by the ratio s4 / Z1, being higher than 3 in Amblydromalus and lower than 3 in Typhlodromalus . According to this criterion, the new species keys to Amblydromalus by having ratio s4 / Z1 = 7.1, but its habitus and main taxonomic features are those of Typhlodromalus . Typhlodromalus annulatus has a strongly ornamented dorsal shield with areolae; the posterior margin of the shield is indented at level of seta S5; most of dorsal setae are stout and placed on tubercles, and macrosetae on leg IV are thick and blunt; all of these are usual characters of Typhlodromalus species. For this reason we consider this species belongs to Typhlodromalus , and we suggest that care should be used in the use of ratio s4 / Z1 as a unique criterion to distinguish these genera. More useful characters for separation include the sculpture of dorsal shield, smooth in Amblydromalus (except for A. insolitus Nuvoloni & Lofego ) and reticulate and rugose in Typhlodromalus ; the posterior margins of dorsal shield, which do not have indentation at level of seta S 5 in Amblydromalus but with a conspicuous indentation in Typhlodromalus (except for T. longisetatus Moraes, Zannou & Oliveira and T. tenuicalyx Moraes, Zannou & Oliveira ) giving to this posterior part a trilobate appearance; dorsal setae are setiform and smooth (except for Z5) in Amblydromalus while are stout and sometimes serrated and placed on tubercles in Typhlodromalus ; and the posterior margin of ventrianal shield, which shows a constriction below anal opening, giving a trilobate aspect in Typhlodromalus , which is absent in Amblydromalus (except in A. macroatrium Moraes, Barbosa & Castro ).

It is interesting to note the presence of an incomplete ring around the major duct of the spermathecal apparatus. This structure has also been depicted in other Euseinii, such as Quadromalus colombiensis Moraes , Denmark & Guerrero, Amblydromalus lailae Schicha and A. insolitus Nuvoloni & Lofego , the latter showing intermediate characters between Amblydromalus and Typhlodromalus . However, to our knowledge, this structure has never been described in the literature.

Typhlodromalus annulatus belongs to the peregrinus species group according to Chant & McMurtry (2005), with seta J1 absent and S5 present. The new species can be separated from T. rosayroi Denmark & Muma, T. marmoreus El-Banhawy and T. feresisimilis Moraes, Barbosa & Castro by the shape of dorsal setae, lanceolate in T. feresisimilis , serrate in T. marmoreus and spatulate in T. rosayroi . Moreover, T. rosayroi has slightly reticulated dorsal shield with few areolas.

Typhlodromalus annulatus differs from T. feresi Lofego, Moraes & McMurty and T. ingae Moraes, Barbosa & Castro by its longer z4 and s4 and shorter Z1 (respectively z 4 30–32/ 32–37; s4 37 –38/50–51; Z 1 28–30/34–35). The new species has smooth dorsal setae, except Z4, Z5 and J5 which are barbed, whereas T. feresi has only Z5 serrated and T. ingae has all setae smooth. Macrosetae on leg IV are broad and blunt in T. annulatus , while they are slightly capitate in T. feresi and knobbed in T. ingae . Furthermore, the calyx in the spermatheca of T. feresi and T. ingae is longer (28–32 and 18–19, respectively) than that of the new species.

Figures

FIGURES 13 – 19. Typhlodromalus annulatus n. sp. 13. Female dorsal shield, 14. Female ventral surface, 15. Female chelicera, 16. Spermatheca (two examples), 17. Female leg IV, 18. Male ventrianal shield, 19. Spermatodactyl.