Pseudomonas aeruginosa

Keskin, Z. B. & Kahraman, H., 2022, Effect of calcium on Pseudomonas aeruginasa and Bacillus cereus metabolites, Brazilian Journal of Biology (e 243189) 82, pp. 1-6 : 2

P. aeruginosa (ATCC 27853) and B. cereus (ATCC 10876) obtained from the ATCC and used this study.

2.3. Growth conditions

Microorganisms were grown and cultivated as follows:

3 mL of 24 h bacterial inoculum (OD 600 = 0.3-0.4) was inoculated into 5 mL growth Nutrient Broth media and agitated at the rate of static for 24 h at 37 °C. Crude extracellular enzyme solutions were prepared by removing the cells by centrifugation at 13.500 rpm and room temperature for 5 min. Supernatant harvested was assayed.The concentrations of calcium based in preliminary experiments; in NB was 0,05 M, 0,15 M and 0,15 M were used in Nutrient Broth.