Yamadazyma ovata C.Y. Chai & F.L. Hui, 2021

Gao, Wan-Li, Li, Ying, Chai, Chun-Yue, Yan, Zhen-Li & Hui, Feng-Li, 2021, New species of Yamadazyma from rotting wood in China, MycoKeys 83, pp. 69-84 : 69

Yamadazyma ovata C.Y. Chai & F.L. Hui sp. nov.

Figure 3

Type.

China, Henan Province, Luoyang City , Song County, in rotting wood from a forest park, September 2019, J.Z. Li & Z.T Zhang (holotype NYNU 191125T, culture ex-type CBS 16655 , CICC 33500) .

Etymology.

The species name Yamadazyma ovata refers to the ovoid cell morphology of the type strain.

Description.

The cells are ovoid to ellipsoid (2-3 × 3-6.5 μm) and occur singly or in pairs after growth in a YM broth for three days at 25 °C (Figure 3A ). Budding is multilateral. After three days of growth on YM agar at 25 °C, the colonies are white to cream-colored, buttery, and smooth with entire margins. After nine days at 25 °C, on a Dalmau plate culture with CM agar, pseudohyphae consisting of elongated cells with lateral buds are formed (Figure 3B ). True hyphae are not observed. Asci or signs of conjugation are not observed on sporulation media. Glucose, galactose, and trehalose are fermented, but maltose, sucrose, melibiose, lactose, cellobiose, melezitose, raffinose, d-xylose, and inulin are not. Glucose, galactose, l-sorbose, d-glucosamine, d-ribose, d-xylose, l-arabinose, d-arabinose, sucrose, maltose, trehalose, methyl α-d-glucoside, cellobiose, salicin, melibiose, melezitose, glycerol, erythritol, ribitol, xylitol, d-glucitol, d-mannitol, d- galactitol, d-glucono-1, 5-lactone, 2-keto-d-gluconate, d-gluconate, succinate, citrate, and ethanol are assimilated. No growth is observed in l-rhamnose, lactose, raffinose, inulin, myo -inositol, d-glucuronate, dl-lactate, or methanol. In nitrogen-assimilation tests, growth is present on l-lysine, creatine, glucosamine, and d-tryptophan, while growth is absent on nitrate, nitrite, ethylamine, cadaverine, creatinine, or imidazole. Growth is observed at 37 °C, but not at 40 °C. Growth in the presence of 16% NaCl with 5% glucose is present, but growth in the presence of 0.01% cycloheximide and 1% acetic acid is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.

Additional isolates examined.

China, Henan Province, Luoyang City, Song County, in rotting wood from a forest park, September 2019, J.Z. Li & Z.T Zhang, NYNU 19116, NYNU 19130.

GenBank accession numbers.

Holotype NYNU 191125T (ITS: MT990560; D1/D2 LSU: MT990559); additional isolates NYNU 19116 (ITS: MZ318442; D1/D2 LSU: MZ318423), and NYNU 19130 (ITS: MZ318424; D1/D2 LSU: MZ318425).

Notes.

We generated sequences for three isolates of Y. ovata , NYNU 191125, NYNU 19116, and NYNU 19130. This new species is phylogenetically most closely related to C. trypodendroni (Figure 1 ). Yamadazyma ovata can be distinguished from C. trypodendroni based on ITS and D1/D2 LSU loci (15/565 in ITS and 8/532 in D1/D2 LSU). Physiologically, Y. ovata can be differentiated from C. trypodendroni based on growth in l-sorbose, d-glucosamine, melibiose, and d-glucono-1, 5-lactone, all of which are positive for Y. ovata and negative for C. trypodendroni (Table 2 ) ( Lachance et al. 2011).

Figures

Figure 1. Maximum likelihood phylogenetic tree of Yamadazyma inferred from the combined ITS and D 1 / D 2 LSU dataset and rooted with Scheffersomyces coipomoensis (CBS 8178) and Babjeviella inositovora (CBS 8006). ML and MP bootstrap support values above 50 % are respectively shown at the first and second positions. Newly sequenced collections are in blue boldface.

Figure 3. Yamadazyma ovata (NYNU 191125, holotype) A budding cells after three days in YM broth at 25 ° C B pseudohyphae on cornmeal agar after nine days at 25 ° C. Scale bars: 10 μm.