Ananteris dorae Botero-Trujillo, 2008

Botero-Trujillo, Ricardo & Flórez, Eduardo, 2011, A revisionary approach of Colombian Ananteris (Scorpiones, Buthidae): two new species, a new synonymy, and notes on the value of trichobothria and hemispermatophore for the taxonomy of the group, Zootaxa 2904 (1), pp. 1-44 : 15-18

Ananteris dorae Botero-Trujillo, 2008

Figures 1 , 10–13 , 34 , 42 , 50 , 56–57 , 62 ; Tables 1, 2

Ananteris dorae Botero-Trujillo, 2008a: 287 , 292–295, 297, figs. 1, 12–21, tables 1–2; Botero-Trujillo 2009: 93, fig. 1; Botero- Trujillo & Francke 2009: 9; Ochoa et al. 2010: 17.

Type material. Holotype female: COLOMBIA: Nariño Department: La Planada Natural Reserve , permanent plot, 01°15’N 78°15’W, 1885 m asl, pitfall trap, 02–04 May 2001, G. Oliva, M. 2369 (IAvH-E 100763) (examined).

Further material examined. COLOMBIA: Nariño Department: 1 adult male, Barbacoas, Path El Barro , Río Ñambi Natural Reserve , 01°17’10’’N 78°04’28’’W, 1397 m asl, UV light, 11 September 2008, J. A. Ochoa, R. Botero-Trujillo, J. P. Botero, J. Souza & O. Tovar (MPUJ-SCO-385) . 1 adult female, 1 juvenile female, Barbacoas, Path El Barro, Río Ñambi Natural Reserve , 01°17’10’’N 78°04’2’’'W, 1397 m asl, UV light, 11 September 2008, O. Tovar, J. Souza, R. Botero-Trujillo, J. A. Ochoa & J. P. Botero (ICN-As-775) . 1 adult female, 3 adult males, Barbacoas, Altaquer, Río Ñambi Natural Reserve , 1440 m asl, UV light, 13 October 2009, E. Flórez & D. Luna (ICN- As-816) . 4 adult females, Ricaurte, Path Alto Cartagena, Farm Nueva Estrella , 01°13’16’’N 77°58’08’’W, 1617 m asl, UV light, 12 September 2008, R. Botero-Trujillo, J. A. Ochoa, J. P. Botero, N. Rosales & C. Sevillano (MPUJ- SCO-386) . 3 adult females, 1 subadult female, Ricaurte, Path Alto Cartagena, Farm Nueva Estrella , 01°13’16’’N 77°58’08’’W, 1617 m asl, UV light, 12 September 2008, N. Rosales, C. Sevillano, R. Botero-Trujillo, J. P. Botero & J. A. Ochoa (ICN-As-776) .

Revised diagnosis. Unique metasomal carinal formula 10:10:10:6:3, with median lateral carinae on segment III less pronounced than others and sometimes apparently absent, ventrosubmedian and median lateral carinae absent on segment IV, and ventrolateral carinae absent on segment V. Other features include: carapace weakly, evenly concave and lacking anteromedian projection ( Fig. 34 ); metasomal segments with abundant and strong granulation that obscures the carinae and make them difficult to distinguish; V 1 and V 2 trichobothria unaligned axially, with V 2 located on an external position in relation to V 1 ( Fig. 42 ); fixed finger trichobothria in the order eb: esb: db: est: et: dt ( Fig. 50 ); dorsal surface of chelicerae with dense and complete reticular pattern ( Fig. 34 ); pectines with 13–14 teeth on males (mode = 13), 12–13 on females (mode = 13); sternite VII with paramedian longitudinal carinae obsolete and incomplete, formed by scarcely aggregated granules that may eventually suggest they are absent; pedipalp hand uniformly yellow and spotless; coxosternal region base color yellow with diffuse brownish pigmentation that is darker and more conspicuous in coxa and coxapophysis of legs I. Hemispermatophore: capsular region with three lobes, the basal armed with three minute spines; flagellum with very short pars reflexa ( Fig. 62 ).

Male. Description based on adult male from ICN-As-816. Measurements: see Table 2. Coloration: general coloration brownish with variegated pigmentation over almost the entire body and appendages (see Figs. 10–11 for MPUJ-SCO-385). Carapace predominantly brown with some yellow spots and stripes; anterior and posterior margins dark-brown; arising posteriorly to each lateral ocular tubercle there is a thin and almost straight yellow stripe that ends behind the median ocular tubercle; median ocular tubercle black, surrounded on its base by thin yellow lines on anterior, anterolateral and posterolateral margins. Chelicerae with yellow coxa; hand with dense and complete brown reticular pattern on dorsal view (see Fig. 34 for MPUJ-SCO-385); fixed and movable fingers entirely brown with reddish teeth. Coxosternal region and genital operculum predominantly yellow, with diffuse brownish pigmentation throughout; pectinal basal piece, pectines and sternite III completely yellow; sternites IV– VI with inconspicuous lateral minute brownish spots; sternite VII with light-brown lateral regions; sternite V with a flattened posterior median hyaline area. Tergites predominantly brown; tergites I– VI each with two paramedian yellow spots in anterior position and two transverse yellow lines converging near the midline (arrow-like); tergite VII predominantly brownish but with large yellow areas; lateral margins of tergites I–VII brown. Metasoma predominantly yellowish to reddish; dorsal intercarinal spaces of segments I–IV with a median brown design wider anteriorly, triangle-like on segments I–III but arrow-like on segment IV; all segments with variegated pigmentation on every surfaces; segment V reddish, darker than the preceding segments. Telson vesicle and base of aculeus yellowish, with one thin brown longitudinal band ventromedially, one large ventrolaterally on each side, and a broad one dorsally; distal half of aculeus red; subaculear tubercle yellowish. Pedipalp femur and patella completely brown, with some slightly lighter regions in all surfaces and trichobothrial pits yellow; coxa and trochanter with variegated pigmentation; chela with hand uniformly yellow, fingers predominantly brown (except basally) with yellow trichobothrial pits. Legs with trochanter and telotarsi predominantly yellow, other segments primarily brown.

Carapace: densely covered with conspicuous rounded granules especially on the brown areas; lateral margins converging anteriorly; anterior margin weakly, evenly concave and lacking anteromedian projection (see Fig. 34 for MPUJ-SCO-385); ocular carinae evident, others inconspicuous; with a median depression anterior to the ocular tubercle; postocular furrow evident; median ocular tubercle low, located on the posterior half of the anterior third of carapace; lateral ocular tubercles each with three evident ocelli.

Chelicerae: with abundant fine white setae on the internal and ventral surfaces; cheliceral dentition characteristic of the family Buthidae ( Vachon 1963) . Movable finger dorsally with two small basal teeth, one median pronounced, one subdistal slightly shorter than the median, and one distal tooth. Ventrally with three strong teeth, one basal, one median, and one distal tooth larger than its dorsal counterpart. Fixed finger dorsally with one basal and one median tooth forming a bicuspid, one subdistal, and one distal tooth. Ventrally with only one subdistal tooth slightly basal to the dorsal subdistal.

Coxosternal region: sternum subpentagonal, with deep median depression and two anterolateral furrows; all the components of this region smooth, with few setae; coxapophyses I–II densely setose anteriorly.

Genital operculum and pectines: genital operculum completely divided longitudinally; pectinal basal piece heart-shaped, slightly longer than wide and deeply notched anteriorly; pectines long, with distal tip of marginal lamella reaching posterior end of coxae IV; count of pieces on the pectines: marginal lamellae 3:3, middle lamellae 7:7, teeth 13:14, fulcra absent.

Sternites: III– VI smooth; VII with conspicuous but weak granulation and paramedian longitudinal carinae obsolete and incomplete, lateral carinae completely absent; III–VII with abundant setae, especially on lateral margins; V with flattened posterior median smooth area; spiracles elongated, approximately twice longer than wide in sternites III–IV and three times longer than wide in V – VI.

Tergites: with similar granulation to that of carapace; axial carina only evident on the posterior half of tergites II– VI; dorsolateral carinae completely absent on tergites I– VI; tergite VII tetracarinate, with incomplete paired dorsolateral and lateral carinae, and median elevation on the position of the axial carina.

Metasoma: with abundant setae; segments I–III with ten carinae (paired ventrosubmedian, ventrolateral, median lateral, dorsolateral and dorsosubmedian carinae); segment IV with six (ventrosubmedian and median lateral carinae absent); segment V with three (ventromedian, paired dorsolateral carinae); ventrolateral and median lateral carinae converge distally in segments I–II; ventrosubmedian carinae not parallel in segment I but arranged into S-shape, separated from each other by a greater distance anteriorly than posteriorly; ventrosubmedian carinae connected to each other and to ventrolateral carinae by a transverse row of granules in segments II–III; all carinae granulose, formed by unconnected granules (except for dorsolateral and dorsosubmedian carinae in segments I–IV whose granules are close together); intercarinal spaces with abundant strong granulation.

Telson: vesicle moderately elongated, smooth dorsally, with three granulose ventral carinae and lateral granulations; subaculear tubercle strong and spine-like; aculeus long and curved.

Pedipalps: femur with five vestigial longitudinal carinae (dorsoexternal, dorsointernal, ventroexternal, ventrointernal and internal median carinae); patella without distinct carinae but with few granules on the position of the dorsointernal carina; chela acarinate; fixed finger with six almost linear rows of granules, being the basal the longest; movable finger with seven rows (including the short apical row). Trichobothriotaxy type A, femur with β configuration ( Vachon 1974, 1975).

Legs: tibia, basitarsus and telotarsus with numerous ventral setae; tibial spur present on legs III–IV; prolateral pedal spur single on legs I–II, bifid on legs III–IV; retrolateral pedal spur present on all legs.

Hemispermatophore: Description based on one specimen from ICN-As-816: Flagelliform, thin and poorly sclerotized. Foot narrow and flat. Pedal flexure inconspicuous but movable. Body very long, slightly wider on basal third. Capsular region with a large basal lobe armed with three minute spines distally, an external lobe, and a distinctly curved and well-developed internal lobe. Flagellum with long pars recta and very short pars reflexa ( Fig. 62 ). Carapace length to hemispermatophore body length ratio = 1:1.24. There was no variation between both hemispermatophores examined.

Female. Holotype described by Botero-Trujillo (2008a).

Variability. Pectinal teeth count: 12 to 13 on females (n = 23; mode = 13); 13 to 14 on males (n = 7; mode = 13). Note that one female has ten teeth on the left pectine, but this low number is not included in the count because such pectine lacks the distal piece of the marginal lamella (anomalous condition). Note also that one male has the left pectine damaged and incomplete, with only four basal teeth, and therefore is excluded from the count. Carination of sternite VII (n = 16): paramedian longitudinal carinae are obsolete in most specimens studied (93.8%), but are apparently absent in the holotype female (6.2%). Total body length (including telson): male 14–16 mm, female 19–25 mm.

Anomalies. One female lacks V 2 trichobothrium in the right hand. See ‘Pectinal teeth count’ in the ‘variability’ section above for further anomalies.

Distribution. So far known only from three localities in Nariño Department ( Fig. 1 ). New locality records: COLOMBIA: Nariño Department: i) Barbacoas ( Figs. 56–57 ); ii) Ricaurte.

Ecological notes. All specimens of A. dorae from ‘Río Ñambi’ and ‘Nueva Estrella’ were hand-captured at night, after detection with UV light when actively moving in the leaf litter or fallen trunks. In the former locality, A. dorae was found in sympatry with A. ochoai sp. nov., Tityus sp. and Teuthraustes sp. In the latter, it was found with Troglotayosicus humiculum Botero-Trujillo & Francke, 2009 , Tityus sp. (presumably Tityus cuellari Lourenço, 1994 ) and Teuthraustes sp. ( Ochoa et al. 2010).

Figures

FIGURE 1. Known distribution of Colombian Ananteris. () Ananteris arcadioi Botero-Trujillo, 2008. () Ananteris columbiana Lourenço, 1991. () Ananteris dorae Botero-Trujillo, 2008. () A. dorae and Ananteris ochoai sp. nov. ()Ananteris ehrlichi Lourenço, 1994. () Ananteris faguasi Botero-Trujillo, 2009. () Ananteris gorgonae Lourenço & Flórez, 1989. () Ananteris leilae Lourenço, 1999. () Ananteris meridana González-Sponga, 2006 (also type locality in Venezuela). () Ananteris myriamae Botero-Trujillo, 2007. () Ananteris solimariae sp. nov. ()Ananteris tolimana Teruel & García, 2007. () Ananteris volschenki Botero-Trujillo, 2009.

FIGURES 10–13. Ananteris dorae Botero-Trujillo, 2008. 10–11. Adult male, dorsal and ventral aspects (MPUJ-SCO-385). 12–13. Adult female, dorsal and ventral aspects (ICN-As-776). Scale bars = 5 mm.

FIGURES 32–35. Anterior margin of carapace and chelicerae. 32. Ananteris arcadioi Botero-Trujillo, 2008, adult female (MPUJ-SCO-383). 33. Ananteris columbiana Lourenço, 1991, adult female (IAvH-E 100779). 34. Ananteris dorae Botero-Trujillo, 2008, adult male (MPUJ-SCO-385). 35. Ananteris ehrlichi Lourenço, 1994, adult male (IAvH-E 100814).

FIGURES 40–47. Right pedipalp hand, distribution of ventral trichobothria. 40. Ananteris arcadioi Botero-Trujillo, 2008, adult female (MPUJ-SCO-383). 41. Ananteris columbiana Lourenço, 1991, adult female (IAvH-E 100778). 42. Ananteris dorae Botero-Trujillo, 2008, adult male (ICN-As-816). 43. Ananteris ehrlichi Lourenço, 1994, adult male (IAvH-E 100814). 44. Ananteris meridana González-Sponga, 2006, adult female (ICN-As-321). 45. Ananteris myriamae Botero-Trujillo, 2007, adult male (MPUJ-SCO-382). 46. Ananteris ochoai sp. nov., holotype male (MPUJ-SCO-388). 47. Ananteris solimariae sp. nov., holotype male (MPUJ-SCO-390). Scale bars = 0.5 mm.

FIGURES 48–51. Right pedipalp chela, distribution of external and dorsal trichobothria. 48. Ananteris arcadioi Botero-Trujillo, 2008, adult female (MPUJ-SCO-383). 49. Ananteris columbiana Lourenço, 1991, adult female (IAvH-E 100778). 50. Ananteris dorae Botero-Trujillo, 2008, adult male (ICN-As-816). 51. Ananteris ehrlichi Lourenço, 1994, adult male (IAvH-E 100814). Scale bars = 1 mm.

FIGURES 56–57. Two views of the collection locality of Ananteris ochoai sp. nov. and Ananteris dorae Botero-Trujillo, 2008 in Río Ñambi Natural Reserve, Nariño Department. Photographs by José A. Ochoa.

FIGURES 60–63. Hemispermatophore in ectal aspect and detail of the capsular region in ventral aspect (left for Ananteris dorae Botero-Trujillo, 2008, inverted; right for others). 60. Ananteris arcadioi Botero-Trujillo, 2008 (ICN-As-774). 61. Ananteris columbiana Lourenço, 1991 (ICN-As-446). 62. Ananteris dorae Botero-Trujillo, 2008 (ICN-As-816). 63. Ananteris ehrlichi Lourenço, 1994 (IAvH-E 100815). Scale bars = 0.5 mm (0.25 mm for A. dorae). Abbreviations: il = internal lobe, el = external lobe, ml = median lobe, bl = basal lobe.