Phytomyza nowakowskiana Beiger

Guglya, Yuliia, 2021, Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of seven new species, Zootaxa 5014 (1), pp. 1-158 : 75

Phytomyza nowakowskiana Beiger

( Figs. 221–225 , 599–605 )

Material examined: Ukraine: Transcarpatia: near Lubnya, Uzhanskyi National Park , 49°00’N, 22°43’E, 15.iii.2020, Yu. Guglya, ex Symphytum cordatum (1♂ 2♀) .

Hosts. Boraginaceae : Symphytum cordatum Waldst. & Kit. ex Willd. , S. tuberosum L. ( Beiger 1975).

Mine. ( Fig. 221, 222 ) The larva forms a brown upper surface secondary blotch mine. Pupation takes place outside the mine with the puparium overwintering.

Puparium. ( Figs. 223–225 ) Orange, silky-shining, 2.5 mm long, with shallow but distinct segmentation; surface finely wrinkled except for wide bands of fine spines. One row of sparse larger brown spines encircles each segment medially. Posterior spiracles set on stout conical protuberances that are entirely separate; brown, glossy, with twenty fine dark brown sessile bulbs set in a flattened oval configuration. Ventral portion of last abdominal segment slightly protruding posteriorly viewed from the side. Anal plate orange, distinctly protruding above the surface of the puparium viewed from the side and directed ventro-posteriorly.

Cephalopharyngeal skeleton. ( Fig. 599 ) Right mouthhook larger than the left, each with hook-like abducted portion directed ventro-posteriorly and bearing two accessory teeth. Intermediate sclerite long, 1.18× as long as maximum height of left mouthhook. The mouthhook, the intermediate sclerite and the pharyngeal sclerite anteriorly are strongly sclerotized; the dorsal and ventral cornua are much less so. The ventral cornu bears a “closed” window located centrally. Indentation index 80.

Female head. ( Figs. 600, 601 ) Brownish-black, with only proboscis yellow; orbit not projecting above eye in profile; 2 orb s, 2 fr s; lunule broad, higher than a semicircle, reaching the level between the posterior fr s and the anterior orb s, with shallow and wide furrow; pped of medium size, flattened anteriorly; gena medially 0.25× as high as maximum height of eye.

Female genitalia. ( Figs. 604, 605 ) Capsule of spermatheca relatively small, 0.19× as high as height of anterior part of oviscape. Spermathecae equal in size, dark brown, flattened oval, wider than high. Internal duct invagination cylindrical, 0.65× as deep as height of spermatheca. Spermathecal duct weakly sclerotized. Ventral receptacle Sshaped, with weakly sclerotized tail that is two-bladed in basal half and looped medially. Body of receptacle suboval with uniformly curved basal connecting tube, strongly sclerotized, equal in diameter with capsule of spermatheca; with opening 0.38× as wide as diameter of spherical part of body.

Distribution. Poland ( Beiger 1975). Ukraine (first record).

Comments. The original description of this species does not include illustrations of the wing and hypophallus, which are provided here ( Figs. 602, 603 ).

Figures

FIGURES 221–235. Figures 221–225: Phytomyza nowakowskiana Beiger; 221–222: mine in Symphytum cordatum leaf; 223: empty puparium viewed from the side; 224: posterior segments of puparium (posterior view); 225: posterior spiracles (posterior view). Figures 226–230: P. pulmonaria Nowakowski, reared from Myosotis sparsiflora; 226: mine; 227: puparium in mine; 228: empty puparium viewed from the side; 229: posterior segments of puparium (posterior view); 230: posterior spiracles (posterior view). Figures 231–235: P. pulmonaria Nowakowski, reared from Symphytum asperum; 231: mines; 232: puparia in mine; 233: empty puparium viewed from the side; 234: posterior segments of puparium (posterior view); 235: posterior spiracles (posterior view).

FIGURES 599–605. Phytomyza nowakowskiana Beiger; 599: cephalopharyngeal skeleton; 600: frontal view of head; 601: head viewed from the side; 602: wing; 603: hypophallus; 604: oviscape, ventral receptacle and spermatheca proportionally; 605 a–b: spermatheca and ventral receptacle proportionally; a: spermatheca; b: ventral receptacle.