Phytomyza medicaginis Hendel

Guglya, Yuliia, 2021, Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of seven new species, Zootaxa 5014 (1), pp. 1-158 : 73-75

Phytomyza medicaginis Hendel

( Figs. 216–220 , 594–598 )

Material examined: Ukraine: Vinnytsa Region: near Chechelnyk, location Vyshenka , 48°10’N, 29°20’E, 16– 31.v.2019, Yu. Guglya, ex Pulmonaria mollis (7♂ 3♀) ; near Olhopil, Lizvora , 48°07’40”N, 29°39’14”E, 10.v.2019, Yu. Guglya (1♂) ; Kharkiv Region: near Rubizhne , 50°07’N, 36°46’E, 23.v.2019, Yu. Guglya, ex Pulmonaria officinalis . (1♂ 1♀) .

Hosts. Boraginaceae : Echium Tourn. ex L., Symphytum Tourn. ex L. ( Benavent-Corai et al. 2005), Brunnera Steven , Lithospermum L. ( Warrington 2021). Pulmonaria mollis Wulfen ex Hornem and P. officinalis L.—newly recorded host plants.

Mine. The larva forms a brown upper surface leaf mine that is initially irregular and linear ( Fig. 216 ), then transforms into a large blotch ( Fig. 217 ). Mines can coalesce. Pupation takes place mainly within the mine on the lower leaf surface.

Puparium. ( Figs. 218–220 ) Orange, silky-shining, 2.3 mm long, with shallow but distinct segmentation; surface quite smooth except for wide bands of fine spines. One row of sparse larger brown spines encircles each segment medially. Posterior spiracles set on stout conical protuberances that are entirely separate; brown, glossy, with numerous fine black sessile bulbs set in a flattened oval configuration. Ventral portion of last abdominal segment strongly protruding posteriorly viewed from the side. Anal plate orange, protruding above the surface of the puparium viewed from the side and directed ventro-posteriorly.

Cephalopharyngeal skeleton. ( Fig. 594 ) Right mouthhook larger than the left. each bearing two accessory teeth. Intermediate sclerite long, 1.12× as long as maximum height of left mouthhook. The mouthhook, the intermediate sclerite and the pharyngeal sclerite in the anterior half are strongly sclerotized; the dorsal and ventral cornua in posterior half are much less so. The ventral cornu bears a “closed” window located posteriorly. Indentation index 86.

Female head. ( Figs. 595, 596 ) Brownish-black, with gena brown, and proboscis yellowish; orbit not projecting above eye in profile; 2 orb s, 2 fr s; lunule broad but higher than a semicircle, reaching the level of the posterior fr s; pped of medium size, narrowed apically; gena medially 0.17× as high as maximum height of eye.

Female genitalia. ( Figs. 597, 598 ) Capsule of spermatheca relatively small, 0.14× as high as height of anterior part of oviscape. Spermathecae equal in size, dark brown, flattened basally and apically, wider than high. Internal duct invagination cylindrical, 0.9× as deep as height of spermatheca. Spermathecal duct weakly sclerotized. Ventral receptacle S-shaped, with weakly sclerotized tail that is two-bladed in basal half. Body of receptacle spherical with uniformly curved basal connecting tube, strongly sclerotized, equal in diameter with capsule of spermatheca; with opening 0.47× as wide as diameter of spherical part of body.

Distribution. Belgium, the Czech Republic, France, Germany, British Isles, Greece, Hungary, Lithuania Poland, Portugal, Romania, Slovakia, Switzerland ( Papp & Černý 2019). Ukraine (first record).

Figures

FIGURES 211–220. Figures 211–215: Phytomyza lycopi Nowakowski; 211: mine in Lycopus europaeus leaf; 212: puparium (indicated by arrow) in mine; 213: empty puparium (lateral view); 214: posterior segments of puparium (posterior view); 215: posterior spiracles (posterior view). Figures 216–220: P. medicaginis Hendel; 216–217: mines in Pulmonaria mollis leaf; 216: initial mine with feeding larva (indicated by arrow); 217: fully developed; 218: empty puparium viewed from the side; 219: posterior segments of puparium (posterior view); 220: posterior spiracles (posterior view).

FIGURES 594–598. Phytomyza medicaginis Hendel; 594: cephalopharyngeal skeleton; 595: frontal view of head; 596: head viewed from the side; 597: oviscape, ventral receptacle and spermathecae proportionally; 598 a–b: spermatheca and ventral receptacle proportionally; a: spermatheca; b: ventral receptacle.