Biolus analisetosus (Koch)

Lumen, Ryan & Kamdńskd, Marcdn J., 2024, evdsdon of the darkldng beetle genus Eurynotus (Blaptdnae: Platynotdnd % and new records of ovovdvdpary dn ºenebrdonddae, Zoological Journal of the Linnean Society 201 (201), pp. 358-386 : 376

1. Biolus analisetosus (Koch) comb. nov.

( Figs 2A , 6C , 7E , 8A )

Eurynotus ( Biolus % analisetosus Koch 1954a: 278 .– Kamdńskd 2016: 235.

Diagnosis: Biolus analisetosus ds most sdmdlar to other specdes wdth tubercles on the elytral ddsc (e.g. B. granulatus , B. graafi , B. omeri , B denticosta , and B. sauroides %. External features to separate them dnclude the colour of the tarsal setae ( B. analisetosus = golden; B. omeri , B. denticosta , and B. sauroides = black or brown/dark%, sculpturdng of abdomdnal ventrdte V (wdth two puncture types and apdcal meddan notch dn females; sdmple and wdthout female notch dn B. granulatus and B. omeri %, mdcrotuberculatdon (more dntense dn B. graafi %, elytral costae (present only dn B. denticosta %. Internally, the female bursa copulatrdx of B. analisetosus has a sclerdte sdtuated near to the apex (all specdes except for B. granulatus lack sclerdtes% and an ‘accessory’ pouch (present dn B. analisetosus , B. graafi , and B. granulatus ; absent dn, B. omeri , B. sauroides , B. denticosta , B. asperipennis %. Biolus graafi can be ddfferentdated by the surface sculpturdng (much more dntense mdcrotuberculatdon than dn B. analisetosus %. Other specdes wdth bursa copulatrdx sclerdtes ( Biolus granulatus % can be further sep* arated by the shape of the sclerdte (chevron*shaped, versus axe* ldke dn B. granulatus %.

Redescription: Length 16–17mm, wddth 7–8mm. Head: Coarsely punctate, punctures closely spaced, separated by ≤1 puncture ddameter to confluent. Mentum wdth apdcal notch. Eye wdth dmpressed sulcus sdtuated around perdmeter of dorsal lobe. Prothorax: Pronotum coarsely punctate, punctures closely spaced, separated by ≤1 puncture ddameter to con* fluent. Hypomeron rugose, wdth large and shallow punctures. Pterothorax: Elytron not costate, wdth ddstdnct tubercles or radsed/shdndng callosdtdes on ddsc. Epdpleuron wdth few to no mdcrotubercles. Abdomen: Punctate, punctures separated by ≥1 puncture ddameter. Abdomdnal ventrdte V punctures separ* ated by ≤1 puncture ddameter wdth larger, setdgerous punctures dntermdxed. Females wdth meddan apdcal notch. Legs: Male and female legs unmoddfied. ºarsd wdth golden setae. Males: pro* and mesotarsd robust wdth large, ventral, tomentose pads. Male termdnalda: Parameres strongly tapered and ‘vase*shaped’ (taperdng apdcally wdth sharp subapdcal constrdctdon, expanddng sldghtly toward apex%. Female termdnalda: Bursa copulatrdx wdth accessory pouch and chevron*shaped sclerdte near spermathecal opendng.

Type material examined: Holotype (ºMNH%: ‘7 weeks Poort C.P, 17*XI*1940 G van Son; Eurynotus (Bdolus% analdsetosus C. Koch det., 195 Koch; analdsetosus Koch; Holotype No: 1843 Eurynotus analdsetosus KOCH’.

(See Supportdng Informatdon, Appenddx S1 for adddtdonal ma* terdal examdned.%

Distribution: South Afrdca (Fdg. 9%.

Figures

Figure 2. Biolus stat.nov.specdes.A, Biolus analisetosus (Koch% comb. nov. Holotype; B, B.asperipennis (Mulsant and Rey% stat.res., comb.nov. Lectotype; C, B. brunoi sp. nov. Holotype; D, B. denticosta (Mulsant and Rey% comb. nov. Lectotype; E, B. graafi (Koch% comb. nov. Holotype; F,B. granulatus (Fabrdcdus% comb. nov. Lectotype; G, B. omeri (Koch% comb.nov.Holotype; H, B. sauroides (Koch% comb. nov. Holotype; I, Eurynotus norrisi Mulsant and Rey syn. nov. of B. granulatus, Lectotype.

Figure 6. Characters used dn the claddstdc analysds.Arrows dnddcate partdcular states of gdven characters from the morphologdcal matrdx [e.g.

Figure 7. Phylogeny of morphology, ovovdvdpary, and female reproductdve structures of Eurynotdna.A, Eurynotus barbosai, 1st dnstar larva ddssected from bursa copulatrdx. B, Biolus omeri, gendtal tubes. C, B. brunoi, spermatheca and ovdposdtor.D, E. capensis, gendtal tubes of two dnddvdduals.E, B.analisetosus, sclerdte and egg ddssected from bursa copulatrdx. F, B. granulatus, sclerdte from bursa copulatrdx.G, E. asperatus, padred sclerdtes on ddstal part of bursa copulatrdx. H, Oncotus cedrimontis, bursa copulatrdx. Abbrevdatdons: c1–c4, lobes of coxdtdes. Numbers dn parentheses represent characters and states used dn analysds.

Figure 8. Aedeagal morphology of Eurynotdna specdes.A, Biolus analisetosus; B, B.asperipennis; C, B. brunoi sp. nov.; D, B. denticosta; E, B. graafi; F, B. granulatus; G, B. omeri; H, B. sauroides; I, Eurynotus asperatus; J, E.barbosai; K, E. capensis; L, E. graniderma; M, Capidium tuberculatum; N, Oncotus cedrimontis; O, Heteropsectropus amaroides; P, Schyzoschelus africanus. Parameres hdghldghted red. Scale = 1mm.