Biolus analisetosus (Koch)

Lumen, Ryan & Kamdńskd, Marcdn J., 2024, evdsdon of the darkldng beetle genus Eurynotus (Blaptdnae: Platynotdnd % and new records of ovovdvdpary dn ºenebrdonddae, Zoological Journal of the Linnean Society 201 (201), pp. 358-386 : 376

1. Biolus analisetosus (Koch) comb. nov.

( Figs 2A , 6C , 7E , 8A )

Eurynotus ( Biolus) analisetosus Koch 1954a: 278 .– Kamiński 2016: 235.

Diagnosis: Biolus analisetosus is most similar to other species with tubercles on the elytral disc (e.g. B. granulatus , B. graafi , B. omeri , B denticosta , and B. sauroides ). External features to separate them include the colour of the tarsal setae ( B. analisetosus = golden; B. omeri , B. denticosta , and B. sauroides = black or brown/dark), sculpturing of abdominal ventrite V (with two puncture types and apical median notch in females; simple and without female notch in B. granulatus and B. omeri ), microtuberculation (more intense in B. graafi ), elytral costae (present only in B. denticosta ). Internally, the female bursa copulatrix of B. analisetosus has a sclerite situated near to the apex (all species except for B. granulatus lack sclerites) and an ‘accessory’ pouch (present in B. analisetosus , B. graafi , and B. granulatus ; absent in, B. omeri , B. sauroides , B. denticosta , B. asperipennis ). Biolus graafi can be differentiated by the surface sculpturing (much more intense microtuberculation than in B. analisetosus ). Other species with bursa copulatrix sclerites ( Biolus granulatus ) can be further sep - arated by the shape of the sclerite (chevron -shaped, versus axe - like in B. granulatus ).

Redescription: Length 16–17mm, width 7–8mm. Head: Coarsely punctate, punctures closely spaced, separated by ≤1 puncture diameter to confluent. Mentum with apical notch. Eye with impressed sulcus situated around perimeter of dorsal lobe. Prothorax: Pronotum coarsely punctate, punctures closely spaced, separated by ≤1 puncture diameter to con - fluent. Hypomeron rugose, with large and shallow punctures. Pterothorax: Elytron not costate, with distinct tubercles or raised/shining callosities on disc. Epipleuron with few to no microtubercles. Abdomen: Punctate, punctures separated by ≥1 puncture diameter. Abdominal ventrite V punctures separ - ated by ≤1 puncture diameter with larger, setigerous punctures intermixed. Females with median apical notch. Legs: Male and female legs unmodified. Tarsi with golden setae. Males: pro - and mesotarsi robust with large, ventral, tomentose pads. Male terminalia: Parameres strongly tapered and ‘vase -shaped’ (tapering apically with sharp subapical constriction, expanding slightly toward apex). Female terminalia: Bursa copulatrix with accessory pouch and chevron -shaped sclerite near spermathecal opening.

Type material examined: Holotype (TMNH): ‘7 weeks Poort C.P, 17-XI -1940 G van Son; Eurynotus (Biolus) analisetosus C. Koch det., 195 Koch; analisetosus Koch; Holotype No: 1843 Eurynotus analisetosus KOCH’.

(See Supporting Information, Appendix S1 for additional ma - terial examined.)

Distribution: South Africa (Fig. 9).

Figures

Figure 2. Biolus stat.nov.species.A, Biolus analisetosus (Koch) comb. nov. Holotype; B, B.asperipennis (Mulsant and Rey) stat.res., comb.nov. Lectotype; C, B. brunoi sp. nov. Holotype; D, B. denticosta (Mulsant and Rey) comb. nov. Lectotype; E, B. graafi (Koch) comb. nov. Holotype; F,B. granulatus (Fabricius) comb. nov. Lectotype; G, B. omeri (Koch) comb.nov.Holotype; H, B. sauroides (Koch) comb. nov. Holotype; I, Eurynotus norrisi Mulsant and Rey syn. nov. of B. granulatus, Lectotype.

Figure 6. Characters used in the cladistic analysis.Arrows indicate particular states of given characters from the morphological matrix [e.g.

Figure 7. Phylogeny of morphology, ovovivipary, and female reproductive structures of Eurynotina.A, Eurynotus barbosai, 1st instar larva dissected from bursa copulatrix. B, Biolus omeri, genital tubes. C, B. brunoi, spermatheca and ovipositor.D, E. capensis, genital tubes of two individuals.E, B.analisetosus, sclerite and egg dissected from bursa copulatrix. F, B. granulatus, sclerite from bursa copulatrix.G, E. asperatus, paired sclerites on distal part of bursa copulatrix. H, Oncotus cedrimontis, bursa copulatrix. Abbreviations: c1–c4, lobes of coxities. Numbers in parentheses represent characters and states used in analysis.

Figure 8. Aedeagal morphology of Eurynotina species.A, Biolus analisetosus; B, B.asperipennis; C, B. brunoi sp. nov.; D, B. denticosta; E, B. graafi; F, B. granulatus; G, B. omeri; H, B. sauroides; I, Eurynotus asperatus; J, E.barbosai; K, E. capensis; L, E. graniderma; M, Capidium tuberculatum; N, Oncotus cedrimontis; O, Heteropsectropus amaroides; P, Schyzoschelus africanus. Parameres highlighted red. Scale = 1mm.