Chthonolpidium Tedersoo, 2025

Tedersoo, Leho, Hosseyni Moghadam, Mahdieh S., Panksep, Kristel, Prins, Victoria, Anslan, Sten, Mikryukov, Vladimir, Bahram, Mohammad, Abarenkov, Kessy, Kõljalg, Urmas, Esmaeilzadeh-Salestani, Keyvan, Pawłowska, Julia, Wurzbacher, Christian, Ding, Yi, Alkahtani, Saad Hussin & Nilsson, R. Henrik, 2025, Thirty novel fungal lineages: formal description based on environmental samples and DNA, MycoKeys 124, pp. 1-121 : 1-121

Chthonolpidium Tedersoo gen. nov.

Type species.

Chthonolpidium enigmatum Tedersoo .

Diagnosis.

Distinguishable from other fungi based on diagnostic nucleotide signatures in ITS 2 (positions 60–74 gggccaagctggtta; one mismatch allowed) and LSU D 2 (positions 672–686 in type species and 604–618 in S. cerevisiae ttgcagttgggcgcc; one mismatch allowed). Forms a monophyletic, least inclusive clade in Chthonolpidiaceae, covering sequences EUK 1124876 and EUK 1138033 (Figs 1 , 39 ).

Notes.

Recognized based on eDNA sequences only. Comprises potentially four species represented by sequences EUK 1124876 (mosses in Estonia), EUK 0534819 (tundra soil in AK, USA), and EUK 0534804 (grassland soil in Tibet).

Figures

Figure 1. Maximum Likelihood SSU- 5.8 S-LSU phylogram indicating placement of novel lineages in the fungal kingdom as highlighted in a coloured shade and red font. Previously described taxonomic clades are collapsed. All branches of Sumavosporidiomycetes and Gelotisporidiomycetes are shortened two-fold. Poorly supported branches with bootstrap values below 95 % / 99 % are indicated in blue. The original tree with bootstrap values and uncollapsed branches is indicated in Suppl. material 1.

Figure 39. Maximum Likelihood SSU- ITS - LSU phylogram indicating the position of Chthonolpidium enigmatum within Chthonolpidiomycetes, with ultra-rapid bootstrap values indicated (for higher-level classifications only). Other genus-level groups are collapsed. Olpidiomycota spp. were used as an outgroup.