Glyptapanteles stephaniekirkae Arias-Penna, sp. nov.

Arias-Penna, Diana Carolina, Whitfield, James B., Janzen, Daniel H., Winifred Hallwachs,, Dyer, Lee A., Smith, M. Alex, Hebert, Paul D. N. & Fernandez-Triana, Jose L., 2019, A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador, ZooKeys 890, pp. 1-685 : 1

Glyptapanteles stephaniekirkae Arias-Penna, sp. nov. Figs 206 , 207

Female.

Body length 2.98 mm, antenna length 3.78 mm, fore wing length 2.88 mm.

Type material.

Holotype: COSTA RICA • 1♀; 07-SRNP-67233, DHJPAR0025771; Área de Conservación Guanacaste, Alajuela, Sector San Cristóbal, Potrero Argentina ; pastures; Malaise ; 520 m; 10.89021, -85.38803; 08.x.2007; DH Janzen & W Hallwachs leg.; ( CNC) . Paratypes. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67114, DHJPAR0025652; same data as for holotype except: 28.xii.2007; ( CNC) . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67119, DHJPAR0025657; same data as for holotype except: 28.xii.2007; ( CNC) . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67128, DHJPAR0025666; same data as for holotype except: 28.xii.2007; ( CNC) . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67129, DHJPAR0025667; same data as for holotype except: 28.xii.2007; ( CNC) .

Other material.

Malaise-trapped material. COSTA RICA: Área de Conservación Guanacaste, Alajuela, Sector San Cristóbal, Bosque Trampa Malaise : • 1 (1♀, 0 ♂) (0 ♀, 0 ♂); 07-SRNP-67444, DHJPAR0025982; 815 m; 10.86280, -85.38460; 02.x.2007; DH Janzen & W Hallwachs leg.

Área de Conservación Guanacaste, Alajuela, Sector San Cristóbal, Rio Blanco Abajo : • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66226, DHJPAR0024764; rain forest; Malaise; 500 m; 10.90037, -85.37254; 08.viii.2007; DH Janzen & W Hallwachs leg. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66306, DHJPAR0024844; same data as for preceding except: 15.viii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66335, DHJPAR0024873; same data as for preceding except: 27.viii.2007 . • 1 (0 ♀, 1♂) (0 ♀, 0 ♂); 07-SRNP-66363, DHJPAR0024901; same data as for preceding except: 02.x.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66395, DHJPAR0024933; same data as for preceding except: 21.viii.2007 . • 1 (0 ♀, 0 ♂) (1♀, 0 ♂); 07-SRNP-66432, DHJPAR0024970; same data as for preceding except: 28.vii.2007 . • 1 (1♀, 0 ♂) (0 ♀, 0 ♂); 07-SRNP-66457, DHJPAR0024995; same data as for preceding except: 19.xi.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66477, DHJPAR0025015; same data as for preceding except: 27.viii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66499, DHJPAR0025037; same data as for preceding except: 16.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂), 07-SRNP-66501, DHJPAR0025039; same data as for preceding except: 16.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66508, DHJPAR0025046; same data as for preceding except: 16.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66515, DHJPAR0025053; same data as for preceding except: 28.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66519, DHJPAR0025057; same data as for preceding except: 28.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66526, DHJPAR0025064; same data as for preceding except: 22.vi.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66535, DHJPAR0025073; same data as for preceding except: 22.vi.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66576, DHJPAR0025114; same data as for preceding except: 04.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66581, DHJPAR0025119; same data as for preceding except: 04.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66603, DHJPAR0025141; same data as for preceding except: 04.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66604, DHJPAR0025142; same data as for preceding except: 04.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66635, DHJPAR0025173; same data as for preceding except: 04.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66646, DHJPAR0025184; same data as for preceding except: 04.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66647, DHJPAR0025185; same data as for preceding except: 04.vii.2007 . • 1 (0 ♀, 1♂) (0 ♀, 0 ♂); 07-SRNP-66695, DHJPAR0025233; same data as for preceding except: 22.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66699, DHJPAR0025237; same data as for preceding except: 22.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66701, DHJPAR0025239; same data as for preceding except: 22.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66749, DHJPAR0025287; same data as for preceding except: 02.ix.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66782, DHJPAR0025320; same data as for preceding except: 16.vi.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66785, DHJPAR0025323; same data as for preceding except: 16.vi.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66802, DHJPAR0025340; same data as for preceding except: 16.vi.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67590, DHJPAR0026285; same data as for preceding except: 03.viii.2007 . • 1 (0 ♀, 0 ♂) (1♀, 0 ♂); 07-SRNP-67596, DHJPAR0026291; same data as for preceding except: 03.viii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67677, DHJPAR0026372; same data as for preceding except: 09.viii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67681, DHJPAR0026376; same data as for preceding except: 09.viii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67693, DHJPAR0026388; same data as for preceding except: 01.xi.2007 . • 1 (1♀, 0 ♂) (0 ♀, 0 ♂); 08-SRNP-2877, DHJPAR0026458; same data as for preceding except: 30.i.2008 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-2898, DHJPAR0026479; same data as for preceding except: 05.ii.2008 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-2983, DHJPAR0026564; same data as for preceding except: 23.ii.2008 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3010, DHJPAR0026591; same data as for preceding except: 29.ii.2008 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3239, DHJPAR0026820; same data as for preceding except: 30.iii.2008 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3335, DHJPAR0026916; same data as for preceding except: 05.iv.2008 . • 1 (0 ♀, 1♂) (0 ♀, 0 ♂); 08-SRNP-3378, DHJPAR0026959; same data as for preceding except: 11.iv.2008 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3408, DHJPAR0026989; same data as for preceding except: 11.iv.2008 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3431, DHJPAR0027012; same data as for preceding except: 11.iv.2008 . • 1 (0 ♀, 1♂) (0 ♀, 0 ♂); 08-SRNP-3460, DHJPAR0027041; same data as for preceding except: 11.iv.2008 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3488, DHJPAR0027069; same data as for preceding except: 17.iv.2008 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3582, DHJPAR0027163; same data as for preceding except: 23.iv.2008 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3645, DHJPAR0027226; same data as for preceding except: 23.iv.2008 . • 1 (1♀, 0 ♂) (0 ♀, 0 ♂); 08-SRNP-3729, DHJPAR0027310; same data as for preceding except: 06.v.2008 . • 1 (1♀, 0 ♂) (0 ♀, 0 ♂); 08-SRNP-3790, DHJPAR0027371; same data as for preceding except: 12.v.2008 . • 1 (1♀, 0 ♂) (0 ♀, 0 ♂); 08-SRNP-3799, DHJPAR0027380; same data as for preceding except: 12.v.2008 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3800, DHJPAR0027381; same data as for preceding except: 12.v.2008 .

Área de Conservación Guanacaste, Alajuela, Sector San Cristóbal, Potrero Argentina: • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67019, DHJPAR0025557, pastures; Malaise ; 520 m; 10.89021, -85.38803; 25.xii.2007; DH Janzen & W Hallwachs leg. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67028, DHJPAR0025566; same data as for preceding except: 04.vii.2007. • 1 (1♀, 0 ♂) (0 ♀, 0 ♂); 07-SRNP-67040, DHJPAR0025578; same data as for preceding except: 04.vii.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67064, DHJPAR0025602; same data as for preceding except: 15.viii.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67076, DHJPAR0025614; same data as for preceding except: 02.x.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67092, DHJPAR0025630; same data as for preceding except: 02.ix.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67111, DHJPAR0025649; same data as for preceding except: 10.vi.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67206, DHJPAR0025744; same data as for preceding except: 04.vii.2007. • 1 (0 ♀, 1♂) (0 ♀, 0 ♂); 07-SRNP-67271, DHJPAR0025809; same data as for preceding except: 21.viii.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67237, DHJPAR0025775; same data as for preceding except: 10.viii.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67734, DHJPAR0027472; same data as for preceding except: 03.viii.2007. • 1 (0 ♀, 1♂) (0 ♀, 0 ♂); 07-SRNP-67742, DHJPAR0027480; same data as for preceding except: 03.viii.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67748, DHJPAR0027486; same data as for preceding except: 03.viii.2007. • 1 (0 ♀, 1♂) (0 ♀, 0 ♂); 07-SRNP-67751, DHJPAR0027489; same data as for preceding except: 03.viii.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67762, DHJPAR0027500; same data as for preceding except: 09.viii.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67794, DHJPAR0027532; same data as for preceding except: 07.xi.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67222, DHJPAR0025760; same data as for preceding except: 16.vi.2007. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3884, DHJPAR0027538; same data as for preceding except: 06.i.2008. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3928, DHJPAR0027582; same data as for preceding except: 06.iii.2008. • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 08-SRNP-3930, DHJPAR0027584; same data as for preceding except: 06.iii.2008 .

Área de Conservación Guanacaste, Alajuela, Sector Rincón Rain Forest, Vado Río Francia: • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-66908, DHJPAR0025446; Malaise ; 400 m; 10.90093, -85.28915; 17.vii.2007; DH Janzen & W Hallwachs leg. • 1 (0 ♀, 1♂) (0 ♀, 0 ♂); 07-SRNP-66936, DHJPAR0025474; same data as for preceding except: 06.vi.2007 . • 1 (0 ♀, 1♂) (0 ♀, 0 ♂); 07-SRNP-67000, DHJPAR0025538; same data as for preceding except: 16.viii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67005, DHJPAR0025543; same data as for preceding except: 05.vii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67568, DHJPAR0026098; same data as for preceding except: 04.viii.2007 . • 1 (0 ♀, 0 ♂) (0 ♀, 1♂); 07-SRNP-67576, DHJPAR0026106; same data as for preceding except: 27.x.2007 . • 1 (0 ♀, 1♂) (0 ♀, 0 ♂); 07-SRNP-67675, DHJPAR0026370; same data as for preceding except: 04.viii.2007 .

Diagnosis.

Petiole on T1 with lateral margins straight throughout, with rugae ( Fig. 206G, H ), propodeum medially rhomboid-shaped with transverse rugae, but no trace of median longitudinal carina ( Fig. 206F ), hind coxa punctate only ventrally, dorsal outer depression present ( Figs 206A, J , 207A ), mesoscutum proximally distinctly punctate, distally with a polished area ( Fig. 206E ), precoxal groove shallow, but visible ( Figs 206A, I , 207A ), fore wing with vein 1 cu-a curved, r vein curved, outer side of junction of r and 2RS veins not forming a stub ( Fig. 207B ), inner margin of eyes diverging slightly at antennal sockets ( Fig. 206B ), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 ( Fig. 206G, H ).

Coloration

( Fig. 206 A–D ). General body coloration satin black except scape yellow-brown with brown areas; brown pedicel distally with a yellow-brown ring; all antennal flagellomeres dark brown on both sides; labrum, mandible, and tegulae yellow; apex of propleuron, both dorsal and ventral furrows of pronotum, epicnemial ridge, and mesopleuron distally with a small area yellow-brown/reddish; glossa, maxillary and labial palps pale yellow/ivory. Eyes and ocelli silver. Fore and middle legs dark yellow, except coxae and trochanters pale yellow/ivory, and claws brown; hind legs pale yellow/ivory except 1/3 proximal of black coxae, femora distally brown, and tibiae and tarsomeres dark yellow/orange. Petiole on T1 with two colorations: distal 3/4 brown and proximal 1/4 lighter yellow-brown/reddish, contours darkened and sublateral areas ivory/pale yellow; T2 with median area dark yellow, adjacent area brown, and lateral ends ivory/pale yellow; T3 medially dark yellow, proximally yellow area coinciding with the width of median and adjacent areas on T2, but distally widen, laterally distal ends with brown coloration; T4 and beyond completely brown; distally each tergum with a wide ivory/pale yellow transparent band. In lateral view, T1-3 ivory/pale yellow; T4 light brown; T5 and beyond brown. S1-3 ivory/yellow; S4 and beyond brown.

Description.

Head ( Fig. 206 A–D ). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.28:0.08, 0.28:0.08, 0.27:0.08), distal antennal flagellomere longer than penultimate (0.15:0.07, 0.12:0.07), antenna longer than body (3.78, 2.98); antennal scrobes-frons sloped and forming a shelf. Face flat or nearly so, punctate-lacunose, interspaces wavy and longitudinal median carina present. Frons smooth. Temple wide, punctate-lacunose and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.09, 0.12). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally pointed or nearly so and dorsally wide.

Mesosoma ( Fig. 206A, E, F, I ). Mesosoma dorsoventrally convex. Distal 1/3 of mesoscutum with lateral margin slightly dented, punctation proximally distinct, but distally with a polished area, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS, scutellar punctation distinct throughout, in profile scutellum flat and on same plane as mesoscutum, but not in the same plane, phragma of the scutellum completely concealed; BS only very partially overlapping the MPM; ATS demilune with a little, complete and parallel carinae; dorsal ATS groove with semicircular/parallel carinae. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation smooth, shiny and sloped. Metanotum with BM upward; MPM circular without median longitudinal carina; AFM without setiferous lobes and not as well delineated as PFM; PFM thick and smooth; ATM proximally with a groove with some sculpturing and distally smooth. Propodeum with transverse rugae, proximal half curved with medium-sized sculpture and distal half rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by a short concave carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron with fine rugae and dorsally with a carina. Metasternum convex. Contour of mesopleuron straight/angulate or nearly so; precoxal groove shallow, but visible and with transverse lineate sculpture; epicnemial ridge convex, teardrop-shaped.

Legs ( Fig. 206A, J ). Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.12, 0.10). Hind coxa with punctation only on ventral surface, dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.32, 0.20), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus longer than fourth tarsomere (0.15, 0.13).

Wings ( Fig. 207B, C ). Fore wing with r vein curve; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral; vein 2 cu-a absent; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe narrow, subdistally evenly convex and subproximally straightened, and setae evenly scattered in the margin.

Metasoma ( Fig. 206A, G, H, J ). Metasoma laterally compressed. Petiole on T1, distal half with faint rugae only laterally, virtually parallel-sided over most of length, but narrowing over distal 1/3 (length 0.36, maximum width 0.18, minimum width 0.10), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.18, length T2 0.18), edges of median area polished and lateral grooves deep, median area broader than long (length 0.18, maximum width 0.21, minimum width 0.07); T2 with scattered pubescence only distally. T3 longer than T2 (0.24, 0.18) and with scattered pubescence only distally. Pubescence on hypopygium dense.

Cocoons. Unknown.

Comments.

The pale coloration on T2 and T3 are distinct.

Male

( Fig. 207 A–C ). Some males exhibit the hind legs darker than females and the brown coloration in the hind coxae covers more area. In all males, the coloration on T2 and T3 is darker than in females, yellow coloration is replaced by light brown; however, in some males the junction between T2 and T3 exhibits yellow-brown/reddish coloration.

Etymology.

Stephanie Kirk is a research technician at the Biodiversity Institute of Ontario ( BIO) in Guelph, Canada.

Distribution.

Adult parasitoids were collected in Costa Rica, ACG, Sector Rincón Rain Forest (Vado Río Francia) and Sector San Cristóbal (Bosque Trampa Malaise, Potrero Argentina, and Río Blanco Abajo), during June-December 2007 and January-May 2008 at 400 m, 500 m, 520 m, and 815 m in pasture and rain forest.

Biology.

Unknown.

Host.

Unknown.

Figures

Figure 206. Glyptapanteles stephaniekirkae sp. nov. female 07 - SRNP- 67233 DHJPAR 0025771 A Habitus B, D Head B Frontal view D Dorsal view C Head, pronotum, propleuron, lateral view E Mesonotum, dorsal view F Scutellum, metanotum, propodeum, dorsal view G T 1 - 3, dorsal view H, J Metasoma H Dorsal view J Lateral view I Mesosoma, lateral view.

Figure 207. Glyptapanteles stephaniekirkae sp. nov. male 08 - SRNP- 3460 DHJPAR 0027041, 07 - SRNP- 66621 DHJPAR 0025159 A Habitus, B, C Wings B Fore C Hind.