Iodosphaeria chiayiensis Marasinghe, C.H. Kuo & K.D. Hyde, sp. nov.

Dissanayake, Lakmali S., Marasinghe, Diana S., Samarakoon, Milan C., Maharachchikumbura, Sajeewa S. N., Mortimer, Peter E., Hyde, Kevin D., Kuo, Chang-Hsin & Kang, Ji-Chuan, 2022, Three new species of Iodosphaeria (Xylariomycetidae): I. chiayiensis, I. jinghongensis and I. thailandica, MycoKeys 86, pp. 1-17 : 1

Iodosphaeria chiayiensis Marasinghe, C.H. Kuo & K.D. Hyde, sp. nov.

Figure 2

Etymology.

The specific epithet Iodosphaeria chiayiensis refers to the city name where the fungus was collected.

Holotype.

MFLU 21-0042.

Description.

Saprobic on dead leaves of an unidentified host. Sexual morph: Ascomata 150-190 × 160-200 μm (x̅ = 170 × 180 μm, n = 10), globose to subglobose, superficial, black, solitary to gregarious, consisting of numerous long, flexuous setae. Setae 3-5 μm wide, arising from cells at the peridium surface, brown, unbranched, septate, apex flattened. Ostiole periphysate, apapillate. Peridium 50-55 μm wide (x̅ = 53.4 μm, n = 10), comprises two layers of textura angularis cells, outer layer of dark brown to black thick-walled cells, and an inner layer of flattened, light brown. Paraphyses 2-4 μm wide, shorter than asci, hyaline, embedded in a gelatinous matrix. Asci 60-90 × 8-10 μm (x̅ = 72.9 × 9.2 μm, n = 30), 8-spored, unitunicate, cylindrical, shortly pedicellate, apex rounded, with a J+ apical ring. Ascospores 15-20 × 4-6 μm (x̅ = 17.2 × 5.2 μm, n = 30), overlapping uni-seriate, ellipsoidal to allantoid, aseptate, hyaline, guttulate. Asexual morph: Undetermined

Material examined.

Taiwan, Chiayi, Fanlu Township area , on dead leaves of an undetermined species, 10 September 2019, D.S Marasinghe, DTF018 (MFLU 21-0042, holotype) .

Notes.

Iodosphaeria chiayiensis resembles I. polygoni which has globose to sub globose, superficial, solitary to gregarious ascomata, cylindrical, short pedicellate asci with J+, apical rings and ellipsoidal to allantoid, aseptate, guttulate ascospores. However, I. chiayiensis differs from I. polygoni in having smaller ascomata (150-190 × 160-200 μm vs. 270-475 × 250-500 μm) and shorter asci (60-90 × 8-10 μm vs. 150-180 × 10-13 μm) ( Hsieh et al. 1997). In the multi-gene phylogenetic analyses (Figure 1 ), our collection ( Iodosphaeria chiayiensis , MFLU 21-0042) has close affinity to I. thailandica. However, it was not possible to compare I. chiayiensis and I. jinghongensis as they occur as different morphs.

Figures

Figure 1. RAxML tree based on a combined dataset of partial LSU and ITS sequence analyses. The tree is rooted to Delonicicola siamense (MFLUCC 15 - 0670) and Furfurella luteostiolata (CBS 143620). Type strains are in bold, and the newly generated strains are in red.

Figure 2. Iodosphaeria chiayiensis (MFLU 21 - 0042, holotype) a substrate b ascomata on the host surface c section of ascoma d appearance of setae on peridium e peridium f, g asci h J + apical ring (in Melzer's reagent) i paraphyses j-m ascospores (m stained in lactophenol cotton blue). Scale bars: 50 μm (c, e); 5 μm (d); 20 μm (f-g); 5 μm (h, i); 10 μm (j-m).