Collybiopsis nonnulla (Corner) R.H. Petersen, Mycotaxon, 2021

Kumla, Wiphawanee Phonrob Jaturong, Suwannarach, Nakarin, Khuna, Surapong, Nutaratat, Pumin, Wannathes, Nopparat & Lumyong, Saisamorn, 2024, Five new records of Collybiopsis (Agaricales, Omphalotaceae) in Thailand: a significant addition to the species diversity and global distribution of the genus, Phytotaxa 650 (3), pp. 213-235 : 229-230

Collybiopsis nonnulla (Corner) R.H. Petersen, Mycotaxon 136(2): 343 (2021) FIGURE 8

Index Fungorum number: IF556194

Pileus 20–26 × 5–15 mm, obtusely conical, convex to plano-convex, glabrous, striate, reddish brown (8E5) at center, brownish grey (5D4) at margin. Lamellar adnate, subdistant (20–22) with 1–2 series of lamellulae, yellowish white, narrow (2 mm), non-intervonose. Stipe 18–25 × 2–3 mm, central, tapering downward, pubescent, insititious, yellowish white at apex, brown (8E5–6) at base; Context thin. Odor and taste not distinctive.

Basidiospore [25/1/1] 8–9 × 3–4 µm [x̄ = 8.38 ± 0.48 × 3.48 ± 0.49 µm, Q = 2–3, Q m = 2.45 ± 0.33] elongate-ellipsoid, smooth, hyaline, inamyloid, thin-walled. Basidia 23–30 × 5–7 µm, clavate, with 4 sterigmata, thin walled. Cheilocystidia scattered, 30–43 × 3–7 µm, clavate, flexuose, irregular in shape, sometimes 2-celled, hyaline, inamyloid, thin-walled, sometimes incrusted. Pleurocystidia absent. Lamellar trama interwoven, hyphae 4–13 µm, cylindrical, smooth, hyaline, inamyloid, thin walled. Pileipellis cutis of radially arranged hyphae 3–6 µm, cylindrical, yellowish brown to light brown, inamyloid, non-diverticulate, incrusted. Pileocystidia 22–65 × 4–6 µm, cylindrical, hyaline, inamyloid, thin walled. Pileus trama subregular, 5–12 µm, cylindrical, branched, smooth, brownish yellow, inamyloid, thin walled. Stipitipellis cutis, 3–8 µm, cylindrical, yellowish brown, smooth, weakly dextrinoid, thick-walled (1–2 µm), non-incrusted. Stipe trama parallel, 3–6 µm, cylindrical, smooth, pale yellow, inamyloid. Caulocystidia abundant, 27–37 × 4–7 µm, cylindrical to clavate, irregular in shape, flexuose, hyaline, inamyloid thin walled. Clamp connections are present in all tissues.

Habitat and known distribution:—Fruiting gregarious to solitary on twig and wood. Known from Cameroon, USA ( Petersen & Hughes 2021), Indonesia (Java) ( Wilson et al. 2004), Korea ( Kim et al. 2022), and Thailand (This study).

Material examined:— THAILAND, Sukhothai Province, Sri Satchanalai National Park, trial to Tarawasan Cave, 99°49´27´´N 17°55´53´´E, elevation 192 m, leaf litter and twig, 19 August 2018, N. Wannathes, J. Kumla, N. Suwannarach, S. Lumyong, and P. Lumyong BKF10189.

Note:—Thai materials have macroscopic and microscopic features that agree well with the descriptions of Desjardin et al. (2004). Thai materials were clustered in the C. nonnulla clade ( FIGURES 2 and 3 ). Morphologically, C. nonnulla is similar to C. menehune . However, C. menehune differs from C. nonnulla by having longer caulocystidia (16–88 µm long) and diverticula of pileipellis. Phylogenetic analyses revealed that C. nonnulla can be clearly distinguished from C. menehune ( FIGURES 2 and 3 ).

Figures

FIGURE 2. Phylogenetic tree derived from maximum likelihood analysis of 95 sequences of the combined ITS and nrLSU genes. Sequences of Rhodocollybia maculata TFB14382 and R. butyracea TFB13989 were used as outgroups. The numbers above the branches represent bootstrap percentages (left) and posterior probabilities (right). Bootstrap values ≥ 75% and posterior probabilities ≥ 0.90 are shown. The scale bar represents the expected number of nucleotide substitutions per site. Sequences of specimen vouchers obtained in this study are highlighted in red. Type species are shown in bold.

FIGURE 3. Phylogenetic tree derived from maximum likelihood analysis of 95 ITS gene sequences.Sequences of Rhodocollybia maculata TFB14382 and R. butyracea TFB13989 were used as outgroups. The numbers above the branches represent bootstrap percentages (left) and posterior probabilities (right). Bootstrap values ≥ 75% and posterior probabilities ≥ 0.90 are shown. The scale bar represents the expected number of nucleotide substitutions per site. Sequences of specimen vouchers obtained in this study are highlighted in red. Type species are shown in bold.

FIGURE 8. Collybiopsis nonnulla (BKF10189). A. Basidiomata; B. Basidiospores; C. Basidia; D. Cheilocystidia; E. Pileipellis; F. Caulocystidia. Scale bars: A = 10 mm; B–D, F = 10 µm; E = 20 µm.