Erioscyphella hainanensis (W.Y. Zhuang and Zheng Wang) Hosoya and Tochihara, 2022

Tochihara, Yukito & Hosoya, Tsuyoshi, 2022, Examination of the generic concept and species boundaries of the genus Erioscyphella (Lachnaceae, Helotiales, Ascomycota) with the proposal of new species and new combinations based on the Japanese materials, MycoKeys 87, pp. 1-52 : 1

Erioscyphella hainanensis (W.Y. Zhuang and Zheng Wang) Hosoya and Tochihara comb. nov.

Lachnum hainanense ≡ Lachnum hainanense W.Y. Zhuang & Zheng Wang, Mycotaxon 67: 25 (1998).

Diagnosis.

Forming apothecia with long stipes and long hairs. Differing E. sinensis in much shorter ascospores.

Japanese name.

Shii-Kashi-hina-no-chawantake.

Specimens examined.

Japan , Niigata, Minamiuonuma, 37.056808, 138.80705, ca 720 m, 14 May 2010, on fallen leaves of Quercus glauca , T.Hosoya (TNS-F-35049). Ibid (TNS-F-35056). Japan, Kanagawa, Hiratsuka, 35.33861111, 139.285, ca 80 m, 12 Apr. 2015, on fallen leaves of Q. myrsinifolia , M.Nakajima (TNS-F-61775). JAPAN, Kanagawa, Kamakura, 35.30756, 139.51958, ca 40 m, 24 Apr. 2015, on fallen leaves of Q. serrata , M.Nakajima (TNS-F-61941). Japan, Gunma, Midori, 36.476684, 139.242771, ca 510 m, 9 May 2016, on fallen leaves of Q. serrata , K.Furuya (TNS-F-65722). Japan, Kanagawa, Hiratsuka, 35.340139, 139.287167, ca 60 m, 18 May 2017, on fallen leaves of Q. glauca , Y.Tochiara (TNS-F-80356). The same locality, on fallen leaves of Castanopsis sieboldii , Y. Tochihara (TNS-F-80371) .

Distribution.

China (Hainan), Japan (Honshu: Kanto region).

Notes.

Based on the UNITE SH system at a 3% threshold, ITS sequences of this species were integrated into a single SH (DOI: SH1155844.08FU). SH1155844.08FU included sequences labeled as ' Hyaloscyphaceae ' (JX984680) in UNITE and ' L. albidulum ' (MK282242) in INSDC (Table 2 ). JX984680 was sequenced from air samples in Seoul, South Korea, and was not tied to any fungal specimens or cultures. Lachnum albidulum is common on leathery dicot leaves of the old and new world tropics ( Haines 1992). Erioscyphella hainanensis resembles L. albidulum in morphology, but L. albidulum has yellow resinous substances at the tip of apothecial hairs and occurs on dead leaves of Rubiaceae ( Haines 1992), whereas E. hainanensis lacks resinous substances and occurs on leaves of broad-leaved trees ( Zhuang and Wang 1998b; Hosoya et al. 2013). Therefore, we presume that MK282242, coexisting with L. hainanense in every SH, was misidentified as L. albidulum . No sequences are available for L. albidulum specimens from the type locality. Lachnum hainanense was therefore judged as acceptable species, and recombined into Erioscyphella .

Erioscyphella hainanensis resembles E. sinensis in occurring on dead leaves of Quercus spp. or Castanopsis spp. However, E. hainanensis has much shorter ascospores than E. sinensis . In this study, presence of minute, hyaline apical amorphous materials and absence of any crystals or resinous materials were confirmed in both species (Suppl. material 1: Fig. S2).