Cleistocybe vernaloides H.M. Wu, J.Q. Luo, Ke Wang & Y.J. Yao, 2018
|
publication ID |
https://doi.org/ 10.11646/phytotaxa.336.3.7 |
|
persistent identifier |
https://treatment.plazi.org/id/AA3487D9-FF80-FFA8-9FB8-FF7BFAD8FA19 |
|
treatment provided by |
Felipe |
|
scientific name |
Cleistocybe vernaloides H.M. Wu, J.Q. Luo, Ke Wang & Y.J. Yao |
| status |
sp. nov. |
Cleistocybe vernaloides H.M. Wu, J.Q. Luo, Ke Wang & Y.J. Yao View in CoL , sp. nov. Figs 1–3 View FIGURE 1 View FIGURE 2 View FIGURE 3
Fungal Names database registration FN570383
Holotype:— CHINA. Qinghai Province, Qilian County, Binggou Linchang, 100°18’45.59”N, 38°02’44.38”E, 3112 m alt., in a forest of Platycladus orientalis ( L.) Franco , mixed with broadleaved trees, 27 August 2015, Ke Wang-180 ( HMAS 0276619 View Materials ).The specimen is deposited in the Fungarium ( HMAS) of the Chinese Academy of Sciences.
GenBank accession numbers:—ITS-nrDNA, KY 622013; LSU-nrDNA, KY 622014.
Species diagnosis:—The new species is molecularly related to C. vernalis , the type of Cleistocybe , but is a different species as revealed by both ITS and LSU-nrDNA sequence analyses. It is also morphologically similar to C. vernalis but differs in having broader basidiospores.
Description:— Pileus 1.0–3.0 cm in diameter, convex to plane; surface dry, not viscid, glabrous, often wrinkled forming reticulate grooves and appearing like large squamules; cartridge buff to pale ochraceous, becoming green in KOH when dry; margin incurved to decurved, with submembranous veil remnants. Lamellae decurrent; up to 2 mm broad, arcuate; close (40‒60 reach the stipe), 1‒2 tiers of lamellulae; near to pale pinkish buff; edge smooth. Stipe 1.5‒ 3.5× 0.5‒0.7 cm, central; cylindrical or slightly attenuate upwards; buff to cinnamon; surface dry, slightly fibrillose; sometimes attached with remaining veil towards the cap, occasionally with a mark of annulus; basal mycelium whitish. Context of pileus 0.2–0.3 cm thick in the center, tapering abruptly to the margin; fleshy; subcolorous close to pileus cuticle. Smell and taste not distinctive.
Basidiospores 8.0‒10.5 × 5.0‒6.5 μm, Q =1.2‒2.1; broadly ellipsoid to ellipsoid to elongate; more or less thin-walled, colorless, sometimes with droplets and granules, inamyloid. Basidia 37‒49‒55 × 7.0‒10.0 μm, clavate; foursterigmate, hyaline to subhyaline, some with granular material. Hymenophoral trama 50–120 μm wide, interwoven to strongly interwoven, more parallel in some areas; hyphae 0.5–2.0 μm diam., thin-walled and hyaline, mostly straight to twisted. Pleurocystidia and cheilocystidia absent. Pileipellis composed of interwoven radiating hyphae 2.0–9.0 μm diam. Stipitipellis longitudinally parallel; hyphae 2.0–6.0 μm diam., hyaline to cinnamon. Clamp connections common in all parts of basidioma.
Comments:—The close relationship between C. vernaloides and C. vernalis , the type species of the genus, is supported both morphologically and molecularly as shown by macroscopic characters, e.g. clitocyboid habitat, decurrent gills and 97% similarity of ITS sequences by Blast search. However, phylogenetic analyses of the ITS sequences demonstrated that C. vernaloides and C. vernalis are segregated in two terminal clades ( Fig. 2 View FIGURE 2 ). The molecular distinction of the two species is also supported by the analysis of LSU sequences as shown in Fig. 3 View FIGURE 3 . Further, the morphological differentiation of C. vernaloides from C. vernalis was revealed by detailed observation. The former is characterized by smaller basidiomata and broader basidiospores. To facilitate the species identification, a key to the known species of Cleistocybe based on morphological characters and occurring seasons is provided below.
No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.