Longipedicellata aptrootii (K.D. Hyde & S.W. Wong) H. Zhang, K.D. Hyde & J.K. Liu, 2016

Zhang, Huang, Dong, Wei, Hyde, Kevin D., Bahkali, Ali H., Liu, Jian-Kui, Zhou, De-Qun & Zhang, Di, 2016, Molecular data shows Didymella aptrootii is a new genus in Bambusicolaceae, Phytotaxa 247 (2), pp. 99-108 : 104-105

Longipedicellata aptrootii (K.D. Hyde & S.W. Wong) H. Zhang, K.D. Hyde & J.K. Liu , comb. nov. (Figs. 2–4)

Index Fungorum number: IF551686; Facesoffunginumber: FoF: 01273;

Basionym:— Didymella aptrootii K.D. Hyde & S.W. Wong, Australas. Mycol. 18 (3): 54 (1999)

Holotypus:— HONG KONG. New Territories. Plover Cove Reservoir, on submerged bamboo, 15 Nov. 1996, K.D. Hyde (IFRD(HKU(M) 3333)!).

FIGURE 3. Longipedicellata aptrootii (from IFRD (HKU 4714)!).A. Appearance of ascomata on submerged wood. B. Section of ascoma. C. Asci with sparse pseudoparaphyses. D. Asci. E–H. Ascospores with mucilaginous sheath. Scale bars: B = 60 μm, C = 60 μm, D = 30 μm, E – H = 10 μm.

Saprobic on submerged bamboo in freshwater. Sexual morph: Ascomata 120-250 μm diam., lenticular, immersed or semi-immersed, beneath a blackened pseudoclypeus, becoming slightly erumpent in host tissue, black, scattered or clustered, coriaceous, ostiolate. Ostiole up to 50 μm, central, short, dark. Peridium less than 5 μm thick, comprising 3 or 4 layers of brown, thin-walled, flattened cells of textura angularis. Pseudoparaphyses absent. Asci 70 – 100 × 17 – 20 μm (x = 84.7 × 19 μm, n = 10), 8-spored, bitunicate, fissitunicate, broadly clavate, pedicel becoming long when released, with an ocular chamber (6.5 μm wide and 2 μm high). Ascospores 17 – 22 × 7 – 10 μm (x = 20.1 × 8.5 μm, n = 20), 2–3-seriate, broad fusiform to ellipsoidal, rounded at both ends, hyaline, 1-septate, asymmetrical, septum slightly nearer to the apex, the upper cell broader and shorter than the lower cell, constricted at the septum, thin-walled, surrounded by large, irregular, mucilaginous sheath. Asexual morph: Undetermined.

Culture characters: —On Potato Dextrose Agar medium, slow growing, reaching 30 mm diameter in 30 days at 25 °C, dark grey from above, with grey zones every 5 – 7 mm, raised, fairly dense, fluffy with aerial mycelium, with a smooth edge, darkened from reverse.

Other material examined: — HONG KONG, New Territories, Plover Cove Reservoir, on submerged bamboo, 15 Nov. 1996, K.D. Hyde ( IFRD ( HKU ( M) 4714)!, isotype) ; Thailand , Chiang Rai, Hui Kang Pla Waterfall, on submerged bamboo, 18 January 2010, Huang Zhang ( MFLU 10-0162 !, reference specimen designated here) ; ex-culture: MFLUCC 10-0297 ; ibid ( MFLU 10-0163 !, MFLU 100164 !) .

FIGURE 4. Culture of Longipedicellata aptrootii (from MFLUCC 100297!). Upper ( A) and reverse ( B) views of colony on PDA 30 days after inoculation. Scale bars: A, B = 1 cm.