Metaphire ryunome Blakemore, 2012

Blakemore, Robert J. & Lee, Seunghan, 2013, Survey of Busan Oligochaeta earthworms supported by DNA barcodes, Journal of Species Research 2 (2), pp. 127-144 : 133-134

8. Metaphire ryunome Blakemore, 2012

( Fig. 4B ).

Material. IV0000261294 (DNA HY2, this data not includ- ed in phylogram Fig. 1 ) collected from Jungang Park, 26 th April, 2013.

Description. Length 116 mm. Clitellum 14-16. Spermathecal pores 7/8/9. Male pores on 18, slightly protruded. GMs absent (cf. Shiga types). Spermathecae with elongated, paprika-shaped diverticular bulbs on long stalks. Prostates present but copulatory pouches absent. Caeca ventrally incised (27rhs removed to check for resident Rotifera that, however, were not found).

Remarks. Slight differences from the original description of Japanese M. ryunome are that the GMs were not recorded and that the caeca were serrate. Nevertheless, DNA shows 100% conformity with barcode results in Blakemore (2012d: fig. 4). Finding M. ryunome in Shiga, Japan and now Busan, Korea indicates that this species has wide distribution suggesting it is peregrine and is to be expected from other countries. The BLAST results (Appendix) show it closest to Chinese A. incongruus (Chen, 1933) on GenBank; however, that taxon typically has spermathecal pores in 5/6/7 rather than 6/7/8 and a different arrangement of GMs, at least, compared to those discussed by Blakemore (2012d) for M. ryunome . Most likely the identity of the Chinese specimen was mistaken, just as the DNA data for A. masatakae appears misidentified with Chinese A. triastriatus (Chen, 1946) on Gen Bank (see Appendix).

LUMBRICIDAE

Figures

Fig. 4. A. Amynthas masatakae (Beddard, 1892) Busan specimen showing spermathecal and male regions. B. Metaphire ryunome Blakemore, 2012 Busan specimen [boxed is X2 enlargement of its18rhs male pore that appears elevated, non-superficial, i.e.,=Metaphire].

Fig. 1. MEGA5 Maximum Likelihood phylogram of DNA code results for Busan specimens compared to similar taxa. Solid squares (■) are Busan samples; open circles (○) are unconfirmed GenBank names (from China, Korea or Japan); closed circles(●) are from the author’s Japanese studies.(Sample codes and provisional or new species names are explained in the Appendix legend).