Cladosporium bambusicola P. P. Costa, A.W.C. Rosado & O.L. Pereira

Costa, Pricila P., Rosado, André W. C. & Pereira, Olinto L., 2022, Six new species of Cladosporium associated with decayed leaves of native bamboo (Bambusoideae) in a fragment of Brazilian Atlantic Forest, Phytotaxa 560 (1), pp. 1-29 : 15-17

Cladosporium bambusicola P. P. Costa, A.W.C. Rosado & O.L. Pereira sp. nov. (Figure 3)

MycoBank: MB824725

Systematic position: Fungi, Ascomycota, Pezizomycotina, Dothideomycetes, Dothideomycetidae, Cladosporiales, Cladosporiaceae

Etymology:—Name refers to the host from which the species was isolated, bamboo.

Colonies on PDA attaining 52 mm diam, dull green, greenish glaucous aerial mycelium, woolly, radially furrowed; sector formation, greenish glaucous, greenish-yellow at the margin, smoke grey aerial mycelium; reverse greenishgrey. Colonies on MEA attaining 50 mm diam, dark green to olivaceous grey, fluffy, woolly; reverse olivaceous black. Colonies on OA attaining 65 mm diam, smoke grey; pale olivaceous grey aerial mycelium, flat, wooly; reverse olivaceous grey. Colonies on SNA attaining 47 mm diam, flesh a rosy vinaceous, hazel, flat, sparse; reverse similar to surface. Mycelium scarce, 1.35–4.72µm wide, septate, sometimes very swollen and constricted at the septum, subhyaline to light brown, sometimes brown, irregularly rough-walled, stromatic hyphal aggregations, and numerous conidia. Conidiophores solitary, macronematous, 23.96–178.99 × 2.40–6.17 µm, arising terminal or laterally from hyphae, erect or flexuous, sometimes seceding at one of the lower septa, cylindrical attenuated towards the apex, sometimes filiform, non-nodulose, rarely geniculate towards the apex, pale brown to brown, sometimes becoming subhyaline towards the apex, 0–10 septate, more or less rough-walled, micronematous, lateral 5.56–21.08 × 2.01–2.78 µm, brown, narrowly cylindrical to filiform, 1–2 loci, 0–1 septum. Conidiogenous cells integrated, mostly terminal, cylindrical, cylindrical-filiform, sometimes geniculate with a small lateral shoulder at apex, 9.13–47.04 × 1.58–4.19µm, brown to pale brown, with 1–4 loci at apex, conidiogenous loci non-protuberant or only slightly so, thickened and darkened. Ramoconidia once or rarely formed, cylindrical-oblong attenuated to apex, 33.90–39.71 × 4.34–5.80µm aseptate, brown, truncate base, unthickened, refractive. Secondary ramoconidia ellipsoid, filiform, oblong-ellipsoid, sometimes subglobose, 1–4 distal hila, slightly thickened, 6.28–26.25 × 2.06–5.51 µm, 0–3 septate, subhyaline, pale brown to sometimes brown. Conidia numerous, catenate, acrogenous, aseptate or once septate, subhyaline to dark brown, intercalary conidia fusiform, ellipsoid-ovoid to subglobose 4.16–10.79 × 2.66–5.27 µm, terminal conidia subglobose to globose, sometimes obovoid, aseptate, 3.29–7.26 × 3.31–5.73 µm. Occurrence of microcyclic conidiogenesis.

Type:— Brazil. Minas Gerais: Araponga, at “ Parque Estadual Serra do Brigadeiro ”, isolated from decayed leaf of Aulonemia amplissima (Poaceae) , March 15, 2016, (holotype VIC 44237, ex-type culture COAD 2256 ) .

GenBank: MT373125 (ACT), MT680204 (TEF1-α), MZ318433 (rDNA-ITS)

Additional specimen examined:— BRAZIL. Minas Gerais: Araponga at “Parque Estadual Serra do Brigadeiro”, isolated from decayed leaves of Aulonemia amplissima (Poaceae) , 15 March 2016 (living culture COAD 2255).

Notes: — Cladosporium bambusicola is a sister group of C. brigadeirensis , but the two species present morphological differences. Cladosporium bambusicola forms two types of intercalary conidia markedly different from each other. One smaller conidia type, fusiform, ellipsoid, ellipsoid-ovoid, ellipsoid-cylindrical, aseptate, subhyaline to pale brown, smooth. Another conidia type is predominantly larger, rarely catenate, subglobose, ellipsoid, limoniform, oblong, oblong-ellipsoid, brown to dark brown, wall rough, thickened. However, it is not possible to differentiate the conidiophore that originated these conidia as in Cladosporium herbaroides ( C. herbarum species complex), which also have two morphological types of conidia. However, they are formed by two different conidiophores, macronematous and micronematous ( Bensch et al. 2012). Cladosporium brigadeirensis does not have ramoconidia and its secondary ramoconidia is on average half the size of C. bambusicola . The conidial chains tend to be larger in the last species, while C. brigadeirensis form a cluster of conidia at the base of conidiogenous cells.