Colletotrichum watphraense X.Y. Ma, K.D. Hyde & Jayawardena

Ma, Xiaoya, Nontachaiyapoom, Sureeporn, Jayawardena, Ruvishika S., yde, Kevin D., Gentekaki, Eleni, Zhou, Sixuan, Qian, Yixin, Wen, Tingchi & Kang, Jichuan, 2018, Endophytic Colletotrichum species from Dendrobium spp. in China and Northern Thailand, MycoKeys 43, pp. 23-57 : 30-32

Colletotrichum watphraense X.Y. Ma, K.D. Hyde & Jayawardena sp. nov. Figure 4

Etymology.

In reference to the host sample site - Wat Phra temple in Chiang Rai, Thailand.

Holotype.

MFLU 17-1202 (ex-holotype culture: MFLUCC 14-0123).

Description.

Sexual morph not observed.

Asexual morph on CMA. Vegetative hyphae 1.6-4.3 µm diam (N=20), smooth-walled, septate, branched, hyaline. Chlamydospores and appressoria not observed. Conidiomata 200 × 300 µm, brown, Conidiophores (15.8-) 18.5-26.8 (-29.1) × (3.4-) 3.8-5.1 (-5.7) µm (N=16), smooth-walled, septate, branched or single, periclinal thickening, hyaline. Conidia (12.4-) 12.5-14.6 (-15.2) × (4.4-) 4.5-5.8 (-6.1) µm (N=5), L/W = 2.3, aseptate, ellipsoidal, single guttules in the middle, the one part inflated, hyaline.

Cultures on CMA flat with entire margin. Growth rate: 0.45cm/day, with 30-days for sporulation. Fluffy, white to light buff orange. Perithecia isolated. Acervuli under white cotton-like mycelia, irregular, asymmetrical surface, light brown to brown.

Material examined.

Thailand, Chiang Rai, Wat Phra That Doi Tung (Temple of Doi Tung Pagodas), the host Dendrobium sp. 2 was collected on 19 December 2013, Collector: Sureeporn Nontachaiyapoom, Natdanai Aewsakul, Xiaoya Ma.

Note.

MFLUCC 14-0123 formed a singular branch with other species and only supported by 1.00BI in boninense species complex. There were 42bp (2.6%) and 85bp (5.2%) differences in GAPDH between Colletotrichum watphraense and its close strains Colletotrichum boninense / C. novae-zelandiae respectively. The closest matches in a blastn search with ITS sequences of the strain MFLUCC 14-0123 are C. cymbidiicola strain FS21 (GenBank KP689224) iaolated from a rare medical plant Huperzia serrata with 99% identity in China ( Wang et al. 2016), C. gloeosporioides strain Trtsf02 (GenBank GU479899) isolated from Trillum tschonoskii with 99% identity in China (Unpublished) and pathogenic C. boninense strain CO5016 (GenBank GU935883) isolated from ginseng with 99% identity in Korea (Unpublished). GAPDH and ACT sequences blastn results showed its closest matches are pathogenic C. citricola strain SXC 151 (GenBank KC293736) isolated from Proteaceae with 99% identity in Netherlands (Liu et al. 2012) and C. boninense strain CBS 125502 (GenBank KJ954462) isolated from Camellia sp. with 99% identity in unknown locality ( Liu et al. 2015). Blastn search with TUB2 sequence results in 99% identity with two C. boninense strains CBS 125502 (GenBank KJ955336) and the strain CGMCC 3.15165 (GenBank KC244156) as mentioned above. The conidiophores were much longer (40 µm long) in C. boninense . Conidia of the strain CBS 123755 have straight, cylindrical to clavate, conidia with a rounded apex; and base with a prominent hilum, sometimes with two large polar guttules, which is different from Colletotrichum watphraense . Here we assigned the strain isolated from stem of Dendrobium sp. 2 as a new species.