Rhodococcus globerulus

Kim, Ju-Young, Jang, Jun Hwee, Maeng, Soohyun & Kim, Myung-Suk Kang and Myung Kyum, 2018, A report of 14 unrecorded bacterial species in Korea isolated in 2017, Journal of Species Research 7 (2), pp. 161-180 : 168-169

Description of Rhodococcus globerulus 17G22-9

Cells are Gram-stain-positive, non-flagellated, and long rod-shaped. Colonies are white-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, N -glucosamine, β -mannosamin, D-fructose, glyctl-L-proline, L-arginine, L-histidine, pyroglutamic acid, Dgluconic acid, methyl pyruvate, L-lactic acid, L-malic acid, succinic acid, γ -butryric acid, α -butryric acid, β - butryric acid, acetoacetic acid, propionic acid, and acetic acid are utilized as sole carbon source. But dextrin, Dmaltose, D-trehalose, D-cellobiolose, gentiobiose, sucrose, D-turanose, stachyose, D-raffinose, α -D-lactose, D-melibiose, β -D-glucoside, D-salicin, N -galactosamin, neuraminic acid, α -D-glucose, D-mannose, D-galactose, 6-methyl-glucose, D-fucose, L-fucose, L-rhamnose, inosine, D-sorbitol, D-mannitol, D-arabitol, myo-Inositol, glycerol, D-glucose, D-fructose, D-aspartic acid, D-serine, gelatin, L-alanine, L-aspartic acid, L-glutamic acid, L-serine, pectin, galacturonic acid, L-galactonic lactone, D-glucuronic acid, glucuronamide, mucic acid, quinic acid, D-saccharic acid, pheylacetic acid, D-lactic acid, citric acid, α -glutaric acid, D-malic acid, tween 40, and formic acid are not utilized. In sensitivity tests, the tetrazolium redox dye is reduced in the presence of pH 6, 1% NaCl, 4% NaCl, sodium lactate, rifamycin SV, nalidixic acid, potassium tellurite, aztreonam, and sodium butyrate but not pH 5, fusidic acid, 8% NaCl, D-serine, troleandomycin, minocycline, lincomycin, guanidine HCl, niaproof 4, vancomycin, tetrazolium violet, tetrazolium blue, lithium chloride, and sodium bromate.

Indole production is negative (API 20NE). In the API 20NE and ID 32GN systems, positive for reduction of nitrates (NO 3) to nitrite (NO 2 -), arginine dihydrolase, urease, esculin hydrolysis, β -galactosidase, L-rhamnose, N -acetyl-glucosamine, D-ribose, D-saccharose (sucrose), suberic acid, lactic acid, D-manitol, salicin, D-melibiose, propionic acid, valeric acid, L-histidine, potassium 2- ketogluconate, 3-hydroxybutyric acid, and 4-hydroxybenzoic acid. Negative for glucose fermentation, gelatin hydrolysis, capric acid, phenylacetic acid, inositol, Dmaltose, itaconic acid, sodium malonate, sodium acetate, L-alanine, potassium 5-ketogluconate, glycogen, 3-hydroxybenzoic acid, L-serine, D-glucose, D-fucose, D-sorbitol, L-arabinose, trisodium citrate, and L-proline.

Strain 17G22-9 (= NIBRBAC000500503) was isolated from a soil sample, Gyeonggi-do, Pocheon-si, Soheuleup, Jikdong-ri, Korea.