Pestalotiopsis aporosae-dioicae C. Z. Yin, Z. X. Zhang & X. G. Zhang, 2024

Yin, Changzhun, Zhang, Zhaoxue, Wang, Shi, Ma, Liguo & Zhang, Xiuguo, 2024, Three new species of Pestalotiopsis (Amphisphaeriales, Sporocadaceae) were identified by morphology and multigene phylogeny from Hainan and Yunnan, China, MycoKeys 107, pp. 51-74 : 51-74

Pestalotiopsis aporosae-dioicae C. Z. Yin, Z. X. Zhang & X. G. Zhang sp. nov.

Fig. 2

Type.

China, Yunnan Province, Jinghong City, Sancha River (22 ° 10 ' 10 " N, 100 ° 51 ' 49 " E), from diseased leaves of Aporosa dioica , 19 Mar 2023, C. Z. Yin, Z. X. Zhang and X. G. Zhang, holotype HMAS 352667 , ex-type living culture SAUCC 224004 .

Etymology.

Referring to the name of the host plant Aporosa dioica .

Description.

Conidiomata in culture on PDA, 600–1000 µm diam, globular, solitary, black conidial masses permeated above the mycelium. Conidiophores mostly degenerated into conidiogenous cells, hyaline. Conidiogenous cells smooth, clavate, hyaline, aggregative, 16.1–22.2 × 3.9–5.5 μm. Conidia fusiform, 4 - septate, slightly curved or straight, 25.6–35.2 × 5.0–7.1 μm; basal cell conical, hyaline, rough, thin-walled, 3.9–9.7 µm; three median cells subcylindrical, light brown or brown, rough, thick-walled, the first median cell from base 4.9–7.0 μm, the second median cell 4.8–7.0 μm, the third median cell 4.6–6.9 μm, together 14.9–20.2 μm; apical cell subcylindrical, hyaline, smooth, thin – walled, 4.7–8.3 µm; basal appendage tubular, single, centric, straight or slightly bent, unbranched, 4.0–13.2 µm; apical appendages tubular, 2–4, straight or bent, unbranched, 8.8–31.7 μm. Sexual morph not observed.

Culture characteristics.

After 14 days of dark cultivation at 25 ° C on PDA, the colony diameter reached 90 mm, and the growth rate is 6.2–6.6 mm / day. Colonies filamentous to circular, aerial mycelium on surface raised, white, dense, forms multiple rings from the middle to the edge, fruiting bodies black; reverse yellow, brown in parts.

Additional specimen examined.

China, Yunnan Province, Jinghong City, Sancha River , from diseased leaves of Aporosa dioica , 19 Mar. 2023, C. Z. Yin, Z. X. Zhang and X. G. Zhang, living culture SAUCC 224005 .

Notes.

According to phylogenetic trees based on ITS, tub 2 and tef 1 α, Pestalotiopsis aporosae-dioicae sp. nov. was closely related to P. arengae in a well support branch (ML / BI = 100 / 1). P. aporosae-dioicae was different from P. arengae by 14 / 508 bp in ITS, 51 / 529 bp in tub 2, and 10 / 465 bp in tef 1 α. Morphologically, P. aporosae-dioicae was different from P. arengae by having thinner conidia ( P. aporosae-dioicae : 25.6–35.2 × 5.0–7.1 vs. P. arengae : 25.0–32.0 × 7.0–9.5 µm) and longer basal appendages ( P. aporosae-dioicae : 4.0–13.2 vs. P. arengae : 1.5–3.0 μm) ( Maharachchikumbura et al. 2014). Therefore, Pestalotiopsis aporosae-dioicae was identified as a new species of Pestalotiopsis by morphological and phylogenetic comparison.

Figures

Figure 2. Pestalotiopsis aporosae-dioicae (holotype: HMAS 352667) a leaves of host Aporosa dioica b, c the front and back of the colony after 14 days of culture on PDA d conidiomata on PDA e, f conidiophores and conidiogenous cells g – m conidia. Scale bars: 10 μm (e – m).