Cryptosporidium, Tyzzer, 1907

Vermeulen, Elke T., Ashworth, Deborah L., Eldridge, Mark D. B. & Power, Michelle L., 2015, Diversity of Cryptosporidium in brush-tailed rock-wallabies (Petrogale penicillata) managed within a species recovery programme, International Journal for Parasitology: Parasites and Wildlife 4 (2), pp. 190-196 : 192

3.1. Cryptosporidium screening

DNA was extracted from 324 samples and screened for Cryptosporidium using 18S rRNA PCR. Of the 324 screened faecal samples, 43 contained the expected amplicon. DNA sequencing and Blast searches identified 23 samples as being Cryptosporidium , giving a total positive identification rate of 7.1% in BTRW. Cryptosporidium positive samples were obtained from three site types (captive bred, supplemented and wild). Positives were found to be present across most study sites except for Jenolan Caves. There was no significant difference in Cryptosporidium detection between captive-bred, wild and supplemented as categories (χ 2 = 3.811, DF = 2, p = 0.149). However, there was a significant difference between the sites (χ 2 = 23.6, DF = 6, p <0.001). Kangaroo Valley Creek had the highest rate of positive samples (40%), but this site had the lowest amount of samples tested (n = 10; Table 1).