Neopestalotiopsis elaeagni Y.K. He & Yong Wang bis, 2022

He, Yu-Ke, Yang, Qi, Sun, Ya-Ru, Zeng, Xiang-Yu, Jayawardena, Ruvishika S., Hyde, Kevin D. & Wang, Yong, 2022, Additions to Neopestalotiopsis (Amphisphaeriales, Sporocadaceae) fungi: two new species and one new host record from China, Biodiversity Data Journal 10, pp. 90709-90709 : 90709

Neopestalotiopsis elaeagni Y.K. He & Yong Wang bis sp. nov.

Materials

Type status: Holotype. Occurrence: recordedBy: Yu-ke He; occurrenceID: GUCC 21002; Taxon : scientificName: Neopestalotiopsis elaeagni; order: Amphisphaeriales ; family: Sporocadaceae ; genus: Neopestalotiopsis ; Location : country: China; stateProvince: Hainan; locality: Haikou City , Leiqiong Haikou Volcano Cluster World Geopark ; verbatimCoordinates: 109°39' E, 20°13' N; Identification: identifiedBy: Yu-ke He; dateIdentified: 2020; Record Level: collectionID: HGUP 10002

Description

Associated with the leaf blight of Elaeagnus pungens Thunb. Disease symptom: A large irregular scab on the leaves of E. pungens , light brown, edges dark brown to reddish-brown. The boundary of the scab was not obvious. There were many black, small and punctuate conidia on the scab. Sexual morph: not observed. Asexual morph (Fig. 2 ): Conidiomata dark, punctiform, scattered on the host scab, 110-300 μm (n = 40), releasing black conidia. Conidiophores discrete to lageniform, hyaline, smooth- and thin-walled, 8-13 × 2-3 μm. Conidia 19-25 × 4.5-7 μm, fusiform to clavate, straight to slightly curved, 4-septate; basal cell obconic with a truncate base, hyaline or pale brown, smooth- and thin-walled, 3.5-5 μm long; three median cells 12-15 μm long, versicoloured, dark brown to light brown, septa and periclinal walls darker than the rest of the cell; second cell brown, 3.5-5.5 μm long; third cell brown, 3-5.5 μm long; fourth cell light brown 3.5-5 μm long; apical cell 3-5.5 μm long, hyaline, conic to acute, with 1-3 tubular appendages inserted at different loci, but in the same crest at the apex of the apical cell, unbranched, flexuous, 13-30 μm long; most conidia have tubular appendages or single appendage in the basal cell, hyaline, unbranched, centric, 5-7.5 μm long.

Culture characteristics: Colonies on PDA medium reaching 5-5.5 cm diam. After 10 d at 24℃, the mycelium white, cottony, odourless, soft, without exudate and round with regular edges. Under the surface of hyphal layer, releasing conidia in a black, slimy mass. The reverse side of the culture dish is smooth and light yellow.

Etymology

Neopestalotiopsis elaeagni , in reference to the host genus ( Elaeagnus ) from which it was isolated.

Notes

Phylogenetically, the new species is sister to Neopestalotiopsis chrysea (MFLUCC 12-0261), Neopestalotiopsis umbrinospora (MFLUCC 12-0285) and Neopestalotiopsis asiatica (MFLUCC 12-0286). However, N. elaeagni differed from N. chrysea by having shorter apical appendage ( N. elaeagni : 13-30 μm vs. N. chrysea : 22-30 μm), differed from N. umbrinospora by having smaller conidia and shorter apical appendage (Conidia: N. elaeagni : 19-25 × 4.5-7 μm vs. N. umbrinospora : 19-29 × 6-8 μm; apical appendage length: N. elaeagni : 13-30 μm vs. N. umbrinospora : 22-35 μm) and differed from N. asiatica by having shorter apical appendage ( N. elaeagni : 13-30 μm vs. N. asiatica : 20-30 μm) ( Maharachchikumbura et al. 2012) (Table 3 ). According to the PHI analysis, our dataset showed a 1.0 value indicating no significant genetic recombination between our newly-introduced Neopestalotiopsis strains with other related taxa. Combined with morphology, phylogenetic analysis and PHI test results and we propose N. elaeagni as a novel species.

Figures

Figure 2. Neopestalotiopsis elaeagni (Specimen code: HGUP 10002). a-c Appearance on host surface; d Colony top view and reverse view; e-f Conidiomata on PDA; g Conidiogenous cells; h-j Conidia. Scale bars: a-b = 10 mm, c = 1 mm, e-f = 500 μm, g-j = 20 μm.