Enterocytozoon bieneusi subsp. genotypes

Yu, Mengshi, Liu, Xue, Karim, Fazal, Xie, Meng, Wu, Jiayun, Li, Diyan, Ni, Qingyong, Zhang, Mingwang, Yu, Guozhi, Xiao, Hongtao, Xu, Huailiang & Yao, Yongfang, 2022, Prevalence and new genotypes of Enterocytozoon bieneusi in wild rhesus macaque (Macaca mulatta) in China: A zoonotic concern, International Journal for Parasitology: Parasites and Wildlife 18, pp. 61-67 : 63

3.2. Molecular diversity of E. bieneusi genotypes

From 38 positive specimens, 11 E. bieneusi genotypes were identified based sequence analysis of the ITS region. Including 3 known genotypes: D (n = 24), EbpC (n = 4) and SCC-2 (n = 1); 8 novel genotypes were following: Mul6 (n = 1), Mul7 (n = 1), Mul8 (n = 1), Mul9 (n = 1), Mul10 (n = 2), Mul11 (n = 1), Mul12 (n = 1) and Mul13 (n = 1) ( Table 1). The base variation of the novel genotypes within the 243 bp of the ITS sequence is presented in Fig. 3 : Mul6, Mul7, Mul9, Mul11, Mul12 and Mul13 contained 1 to 4 single nucleotide polymorphisms (SNPs) with genotype D (MT796858); Mul8 had 3 SNPs relative to genotype PL9 (MT497898); Mul10 had 2 SNPs with genotype CAF4 (MZ502643). Genotype D (63.2%) was the most prevalent, it was observed in 7 populations except XZ-2 and QH. The population SC-1 had another known genotypes SCC2. Genotype EbpC was only found in XZ-2. Distribution of known and novel genotypes have shown in Table 1.

Figures

Fig. 3. Sequence variation in the ITS region of the rRNA gene of Enterocytozoon bieneusi isolates from rhesus macaque. The ITS sequences of 5 known genotypes (D, PL9, CAF4, EbpC, and SCC-2) and 8 novel genotypes (Mul6 to 13) identified in this study, were aligned with each other. The dots and transverse lines indicate base identities and deletions, respectively, relative to the ITS sequence of genotype D.