Pestalotiopsis rhaphiolepidis C. Z. Yin, Z. X. Zhang & X. G. Zhang, 2024

Yin, Changzhun, Zhang, Zhaoxue, Wang, Shi, Ma, Liguo & Zhang, Xiuguo, 2024, Three new species of Pestalotiopsis (Amphisphaeriales, Sporocadaceae) were identified by morphology and multigene phylogeny from Hainan and Yunnan, China, MycoKeys 107, pp. 51-74 : 51-74

Pestalotiopsis rhaphiolepidis C. Z. Yin, Z. X. Zhang & X. G. Zhang sp. nov.

Fig. 4

Type.

China, Hainan Province, Jianfeng Town (18 ° 42 ' 35 " N, 108 ° 52 ' 35 " E), from diseased leaves of Rhaphiolepis indica , 11 Apr 2023, C. Z. Yin, Z. X. Zhang and X. G. Zhang, holotype HMAS 352669 , ex-type living culture SAUCC 367701 .

Etymology.

Referring to the name of the host plant Rhaphiolepis indica .

Description.

Conidiomata in culture on PDA, 600–1000 µm diam, globular, solitary, black conidial masses permeated above the mycelium. Conidiophores mostly degenerated into conidiogenous cells, simple, hyaline. Conidiogenous cells fusiform, rough, discrete, 9.8–17.1 × 2.4–3.3 μm. Conidia fusiform, straight or slightly curved, 4 - septate, 18.0–23.1 × 3.8–5.1 μm; basal cell conical, hyaline, rough, thin-walled, 3.3–5.1 µm; three median cells subcylindrical, light brown or brown, rough, thick-walled, the first median cell from base 3.0–4.7 μm, the second median cell 3.4–5.3 μm, the third median cell 3.7–5.6 μm, together 10.1–15.6 μm; apical cell subcylindrical or conical, hyaline, smooth, thin-walled, 2.8–4.7 µm; basal appendage tubular, single, centric, straight or slightly bent, unbranched, 4.7–9.8 µm; apical appendages tubular, 2–3, straight or bent, unbranched, 5.2–18.5 μm. Sexual morph not observed.

Culture characteristics.

After 7 days of dark cultivation at 25 ° C on PDA, the colony diameter reached 90 mm, and the growth rate is 11.8–13.5 mm / day. Colonies filamentous to circular, flat, center raised, aerial mycelium on surface, with irregular edges, white, medium dense, fruiting bodies black; reverse white, multilayer rings from the middle to the edge.

Additional specimen examined.

China, Hainan Province, Jianfeng Town , from diseased leaves of Rhaphiolepis indica , 11 Apr 2023, C. Z. Yin, Z. X. Zhang and X. G. Zhang, living culture SAUCC 367702 .

Notes.

According to phylogenetic trees based on ITS, tub 2 and tef 1 α, Pestalotiopsis rhaphiolepidis sp. nov. was closely related to P. inflexa in a well support branch (ML / BI = 98 / 1). P. rhaphiolepidis was different from P. inflexa by 9 / 508 bp in ITS, 30 / 529 bp in tub 2, and 16 / 465 bp in tef 1 α. Morphologically, P. rhaphiolepidis was different from P. inflexa by having shorter and thinner conidia ( P. rhaphiolepidis : 18.0–23.1 × 3.8–5.1 vs. P. inflexa : 24.0–31.0 × 6.0–9.0 µm) and shorter apical appendages ( P. rhaphiolepidis : 5.2–18.5 vs. P. inflexa : 20.0–30.0 μm) ( Maharachchikumbura et al. 2011). Therefore, Pestalotiopsis rhaphiolepidis was identified as a new species of Pestalotiopsis by morphological and phylogenetic comparison.

Figures

Figure 4. Pestalotiopsis rhaphiolepidis (holotype: HMAS 352669) a leaves of host Rhaphiolepis indica b, c the front and back of the colony after 14 days of culture on PDA d conidiomata on PDA e – g conidiogenous cells and conidia h – n conidia. Scale bars: 10 μm (e – n).