Torula masonii Crous. IMA Fungus 6(1): 195 (2015)

Wang, Wen-Peng, Shen, Hong-Wei, Bao, Dan-Feng, Lu, Yong-Zhong, Yang, Qiu-Xia, Su, Xi-Jun & Luo, Zong-Long, 2023, Two novel species and three new records of Torulaceae from Yunnan Province, China, MycoKeys 99, pp. 1-24 : 1

Torula masonii Crous. IMA Fungus 6(1): 195 (2015)

Fig. 4

Description.

Saprobic on dead Artemisia carvifolia stems. Sexual morph Undetermined. Asexual morph: Colonies effuse on the natural substrate, scattered, hairy, dark brown to black. Mycelium mostly immersed. Conidiophores 16-28 (-45) × 3-4 μm (x̄ = 26 × 4 μm, n = 10), macronematous mononematous, subcylindrical, erect, septate, smooth, straight or slightly flexuous, brown to dark brown, the uppermost side of a transverse compartment is concave inward. Conidiogenous cells 8-10 × 5-7 μm (x̄ = 9 × 6 μm, n =15), holoblastic, polyblastic, doliiform to ellipsoid, dark brown, smooth. Conidia (16-) 25-48 (-70) × 6-10 μm (x̄ = 35 × 8 μm, n = 50), phragmosporous, in branched chains, acrogenous, dry, brown to dark brown, subhyaline at terminal cell, and central cells are significantly larger than both end cells, nearly ellipsoid, constricted at septa, verrucose, easily separating, 2-16-septate, cells subglobose.

Culture characteristics.

Conidia germinating on PDA within 12 h. and germ tubes produced at the side. Mycelium superficial, branched, septate, hyaline, smooth. After two weeks of incubation at room temperature, colony edges are irregularly ellipsoid, center is white with gray fuzzy protrusions on the sides, and a translucent gelatinous substance at the outermost periphery. Hyphae flocculent, velvety.

Material examined.

China, Yunnan Province, Dali, Cangshan mountain (25°64′82.95″N, 100°15′80.33″E), on dead Artemisia carvifolia stems, 16 October 2021, Wen-Peng Wang H 630 (KUN-HKAS 124616), living culture, KUNCC 22-12429 = CGMCC 3.23734 .

Notes.

Torula masonii collected on Brassica sp. in the UK was introduced by Crous et al. (2015). Since then, it was reported from freshwater and terrestrial habitats in China and Italy ( Li et al. 2017; Su et al. 2018; Tian et al. 2023). In this study, our new collection was obtained from dead stems of Artemisia carvifolia ( Asteraceae ) in Yunnan, China. Phylogenetic analyses showed that our new isolate clustered with the ex-type strain of T. masonii (CBS 245.57) with good bootstrap support (82% ML, Fig. 1 ). Morphologically, our new isolate is similar to T. masonii in having macronematous, mononematous, subcylindrical conidiophores, polyblastic conidiogenous cells and dry, constricted at septa, verrucose, easily separating conidia that are formed branched chains. Thus, we identify this isolate as T. masonii , which was first reported on Artemisia carvifolia ( Asteraceae ).

Figures

Figure 1. The maximum likelihood (ML) tree based on combined LSU, ITS, SSU, tef 1 - α, and rpb 2 sequence data. Bootstrap support values with an ML greater than 75 % and Bayesian posterior probabilities (PP) greater than 0.95 are given above the nodes, shown as " ML / PP ". The tree is rooted with Occultibambusa bambusae (MFLUCC 13 - 0855) and Neooccultibusa thailandensis (MFLUCC 16 - 0274). New species and collections are indicated in red; while the type strains are in bold black.

Figure 4. Torula masonii (KUNCC 22 - 12429) A fungal structure on the substratum C-F conidiophore with conidiogenous cells B, G-O conidia P germinating conidium Q, R colonies on PDA from surface and reverse. Scale bars: 20 μm (B), 10 μm (C-N), 25 μm (O, P)