Lopesia chapadensis, Garcia & Urso-Guimarães, 2018

Garcia, Carolina de Almeida & Urso-Guimarães, Maria Virginia, 2018, Three new species of Lopesia Rübsaamen (Diptera: Cecidomyiidae) from Brazil, Florida Entomologist 101 (2), pp. 203-211 : 204-208

Lopesia chapadensis sp. nov. Garcia & Urso-Guimarães ( Figs. 13–20 )

DESCRIPTION

Adult. Male – Light brown. Body: 1.5 mm long (male, n = 2); 1.7 mm long (female, n = 1). Head: Eyes black, holoptic, facets circular, closely adjacent. Occipital process present. Frontoclypeus with 8–10 long setae; labrum triangular with 3 pairs of setae; hypopharynx of the same shape as labrum; labella elongate-convex, each with 8–10 long lateral setae; palpi total length, 0.1 mm, 4-segmented. Antennae: Total length, 0.9 mm (male); scape and pedicel, 0.06 mm long and maximum wide; scape broader distally; 12 binodal tricircumfilar flagellomeres; circumfila whorls regular in length ( Fig. 13 ). Female flagellomeres cylindrical with interconnected circumfila ( Fig. 14 ); apical process present (as in Fig. 23 ); setulose necks in both sexes. Thorax: Scutum and scutellum dark brown. Scutum with 2 rows of dorsocentral setae, anepimeron with 14 setae, laterotergite with a pair of trichoid sensilla near the halter base, other sclerites bare. Legs: First tarsomere without spur; tarsal claws 1-toothed and bent near midlength, empodia reaching bend in claws ( Fig. 15 ). Wings (as in Fig. 5 ): 1.2 mm long and 0.5 mm wide in male, and 1.6 mm long and 0.5 mm wide in the female. Abdomen: Tergites 1–7 rectangular, completely sclerotized, and a complete row of posterior setae in male and female; tergite 8 not sclerotized in males and female. Trichoid sensilla present at tergites 6–7 in males and absent in females. Sternites 1–8 sclerotized in males and with a complete row of posterior setae. Sternites 8–9 not sclerotized in the female. Male terminalia ( Fig. 16 ): Gonocoxites narrow and with mesobasal lobe rounded and discrete, with setae placed only on external surface; gonostylus clavate, shorter than gonocoxite and sparsely covered with setae and microsetulae, group of 8 sensillae at basal region; gonostylus teeth entire and discrete; cercus bilobed and triangular-shape with straight apex; hypoproct deeply bilobed and setose, with apex rounded; each lobe with few strong setae at tip; parameres absent, aedeagus wide and elongated; rounded at apex and 1.5 longer than hypoproct. Ovipositor (as in Fig. 7 ): 0.08 mm long; slightly protrusible, cerci separate, ovoid and setose, 2 setae longer than the others; hypoproct short and covered by setulae.

Pupa. Dark brown. Body: 1.4 mm long (n = 1). Head: Antennal horns, 0.07 mm long, triangular, sclerotized at the base; upper horn present ( Fig. 17 ). Due to the developed pupal stage, some structures were not visible such as cephalic setae, and facial lower and lateral papillae. Thorax: Wing reaching third abdominal segment; foreleg and midleg reaching third abdominal segment and hind leg reaching sternite 8. Prothoracic spiracle cylindrical, 0.16 mm length ( Fig. 17 ). Abdomen: Abdominal tergites with dorsal spines absent. Integument smooth. Terminal segment 0.15 mm long; 0.1 mm wide.

Larva 3rd instar. Cream. Body length, 1.4 mm (n = 1). Spatula 2-toothed with long sclerotized stalk, length 0.15 mm, enlarged in the posterior region ( Fig. 18 ). Lateral papillae in 2 groups of 3 papillae without setae. Terminal segment with 2 corniform papillae each side, ventral anus in clef ( Fig. 19 ).

Types. HOLOTYPE male, Chapada dos Guimarães (Parque Nacional Chapada dos Guimarães), Mato Grosso, Brazil ( Vale do Eco , 15.4093°S, 55.8330°W), reared from leaf galls of A. vermifuga , 10 Jul 2012 by Maria Virginia Urso-Guimarães , emerged 15 Jul 2012 (Museu de Zoologia of the Universidade of São Paulo, São Paulo, Brazil) . PARATYPES, 1 male, 1 female, 1 pupal exuvia, 1 larva collected and reared with holotype ( Museu de Zoologia of the Universidade of São Paulo, São Paulo, Brazil) .

Etymology. The specific epithet refers to the type locality of the new species, the Chapada dos Guimarães municipality.

Gall and biology. Globoid shape, smooth, light green, unilocular leaf gall on A. vermifuga ( Fig. 20 ). Pupation occurs in the gall.

Remarks. Running the key key to segregation of Lopesia species ( Rodrigues & Maia 2010), we observed that the specimens studied do not belong to any species described, although L. chapadensis sp. nov. shares with L. caulinaris Maia , and L. mataybae sp. nov. the setulose flagellomere necks (couplet 1), narrow gonocoxites (couplet 2’), and male flagellomere gynecoid (couplet 7); wing with Rs joining R 1 afer its midlength (couplet 8) with L. elliptica Maia, 2003 ( Madeira et al. 2003), tarsal claws 1-toothed with (couplet 8’) with L. maricaensis Rodrigues & Maia, 2010 ( Rodrigues & Maia 2010); presence of larval prothoracic spatula with 2 teeth (couplets 1, 2’, and 3), terminal segment of larva without reentrancy and terminal papillae equally long (couplets 4 and 5’), posterior margin of larval prothoracic spatula large and teeth of prothoracic spatula spaced apart, with lateral process and rounded at apex (couplets 7, 8’, and 9) with L. linearis Maia, 2003 .

Despite those similarities, the following set of characteristics differentiates L. mataybae sp. nov. from its congeners: male cerci triangular and bilobed with rounded apex; aedeagus large and wide, larval terminal segment with 2 corniform papillae each side (as L. davillae Maia ) and inducing galls on A. vermifuga .

Figures

Figs. 13–16. Lopesia chapadensis sp. nov. (13). Third male flagellomere. (14). Third female flagellomere. (15). Tarsal claw and empodia. (16). Male terminalia (dorsal view).

Figs. 17–19. Lopesia chapadensis sp. nov. (17). Pupal head (ventral view). (18). Larval prothoracic spatula. (19). Larval terminal segment (dorsal view).

Fig. 20. Leaf galls of Lopesia chapadensis sp. nov. on Andira vermifuga (Fabaceae). Arrow in gall.

Figs. 21–25. Lopesia ubatubensis sp. nov. (21). Third male flagellomere. (22). Third female flagellomere. (23). Twelfh male flagellomere. (24). Tarsal claws and empodia. (25). Male terminalia (dorsal view).

Figs. 1–7. Lopesia mataybae sp. nov. (1). Third male flagellomere (frontal view). (2). Third female flagellomere (frontal view). (3). Twelfh male flagellomere with apical process (frontal view). (4). Male tarsal claws and empodia. (5). Male wing. (6). Male terminalia (dorsal view). (7). Female postabdomen (lateral view).