Phaeoisaria sedimenticola X.L. Cheng & Wei Li

Xu, Ke, Xu, Rong-Ju, Li, Jia-Ning, Zhao, Qi & Zhu, Ying-An, 2024, A novel species of Phaeoisaria (Pleurotheciaceae, Sordariomycetes) from the Tibetan Plateau, China, Phytotaxa 642 (1), pp. 61-72 : 67

Phaeoisaria sedimenticola X.L. Cheng & Wei Li ter, Mycotaxon 127: 20 (2014) FIGURE 4

Index Fungorum number: 563661; Facesoffungi number: FoF 14963

Saprobic on decaying submerged wood in a freshwater stream. Asexual morph: Colonies effuse, scattered, solitary, pale brown to dark brown, hairy or velvety, covered by white conidial mass. Mycelium is partly superficial, partly immersed in the substrate, consisting of branched, septate, smooth, subhyaline to pale brown hyphae. Synnemata 455–710 × 10–20 μm (x = 590 × 15 μm, n = 10), macronematous, branched, synnematous, septate, straight or slightly flexuous, brown to dark brown, smooth. Conidiogenous cells 16–40 × 2–4 μm (x = 27 × 3 μm, n = 10), polyblastic, hyaline to pale brown, sympodial, terminal, cylindrical, denticulate. Conidia 5–7 × 3–4 μm (x = 6 × 3 μm, n = 20), ellipsoidal to obovoid, hyaline to pale brown, guttulate, smooth-walled, aseptate. Sexual morph: Undetermined.

Culture characteristics: Conidia germinated on PDA within 48 hours, and germ tubes were produced from both ends. Colony reached 10 mm at room temperature for one week, circular, flat, with fluffy, dense, raised mycelium in the center.

Material examined: CHINA, Xizang, Motuo County, saprobic on decaying submerged wood in a freshwater stream, 12 July 2022, R.J. Xu, LJR-8 ( HKAS 128986), living culture, KUNCC 10456.

Notes: Cheng et al. (2014) introduced Phaeoisaria sedimenticola from surface marine sediment in an intertidal zone in Shandong, China. Morphologically, our collections are consistent with P. sedimenticola . Phylogenetic analyses showed that P. sedimenticola ( KUNCC 10456) clustered into a clade with an ex-type strain of P. sedimenticola ( CGMCC 3 14949) with 100% ML /1.00 PP bootstrap support ( FIGURE 1 ).

Figures

FIGURE 1. The phylogenetic tree obtained from RAxML analysis with combined ITS, LSU, and SSU sequence data. Bootstrap support values for ML equal to or greater than 75% and PP for BI equal to or greater than 0.95 were indicated at the nodes. Pleurotheciella rivularia (CBS 125237) and Pl. rivularia (CBS 125238) were selected as the outgroup taxa. The new isolates are in blue, and the type species are in bold.

FIGURE 4. Phaeoisaria sedimenticola (HKAS 128986). a Colony on a natural substrate. b Conidiophore and Conidiogenous cell. c, d Conidiogenous cell. e–g Conidiogenous cell with developing conidia. h–m Conidia. n, o Colony on PDA. Scale bars: b = 100 μm, c, d = 10 μm, e–g = 20 μm, h–m = 5 μm.