Stemphylium amaranthi Y.F. Pei & X.G. Zhang, Mycotaxon

Poursafar, Alireza, Ghosta, Youbert & Javan-Nikkhah, Mohammad, 2018, An emended description of Stemphylium amaranthi with its first record for Iran mycobiota, Phytotaxa 371 (2), pp. 93-101 : 98

Stemphylium amaranthi Y.F. Pei & X.G. Zhang, Mycotaxon 109: 495, 2009 ( Fig. 2 )

Colonies on PDA (7 days, in darkness at 24±1 °C) were floccose, 68 mm diam., pale brown to grey at center and creamy to white at margin ( Fig. 2a ); on PCA (7 days, white fluorescent light, 24±1 °C) were smooth, 64 mm diam., olive to dark green with distinct concentric zones of growth and sporulation ( Fig. 2b ). Sporulation was abundant from aerial mycelia and less from submerged mycelia. Hyphae were pale brown, septate, branched, 4–5 μm wide. Conidiophores were pale brown, straight or somewhat curved, up to 200 μm in length. Conidiogenous cells were swollen at the apex, occasionally with 1–5 apical proliferation, pale brown and 5.5–6.5 μm wide. Conidia were pale to dark brown, solitary, spherical to oblong-spherical, cylindrical, bottle shaped, conical or obclavate, rounded at the base and spherical to conical at the apex, sometimes with a short apical extension, usually with 1–7(–9) transverse septa, 1–5 longitudinal septa, 1–4 oblique septa, distinctly constricted at the median septum and 21–57 × 10–18 μm (av. 35.28 × 14.62 μm; L/W= 1.43–3.8) ( Fig. 2c–f ). Sexual morph was firstly formed on PCA plates incubated at 4 °C after 1 month and was fully developed after 2–3 months. Ascomata were dark brown, thick-walled, spherical, 300–700 × 250–550 μm ( Fig. 2g ). Asci were bitunicate, hyaline, 8-spored, 300–350 × 35–37.5 μm ( Fig. 2h–j ). Ascospores were pale to dark brown, with even darkened septa, oblong-spherical, rounded at both ends, with 6–7 transverse septa, 6–7 longitudinal septa, and 34–41 × 14–18 μm (av. 37.44 × 16.83 μm; L/W= 2.11–3.45) ( Fig. 2k–l ).

Specimens examined — IRAN. Golestan Province: Bandar-e Torkaman , on wheat head, May 2014, IRAN 2601C ( ITS MG397180 , GAPDH MG397170 ) ; Golestan Province, Gonbad-e Kavous , on barley head, May 2014, IRAN 2603C ( ITS MG397183 , GAPDH MG397169 ) ; Golestan Province, Gorgan , on wheat head, May 2014, ABRII 10289 ( ITS MG397181 , GAPDH MG397171 ) ; Golestan Province, KordKuy , on barley head, May 2014, ABRII 10286 ( ITS MG397179 , GAPDH MG397172 ) and UTFC –823 ( ITS MG397176 ) ; Golestan Province, Ali Abad-e Katul , on wheat head, May 2014, UTFC –822 ( ITS MG397175 ) ; Golestan Province, Tuskestan , on wheat head, May 2014, UTFC –825 ( ITS MG397178 ), UTFC –821 ( ITS MG397174 ) and UTFC –824 ( ITS MG397177 ) ; Qazvin Province, Abyek , on wheat head, July 2014 ; UTFC –820 ( ITS MG397182 , GAPDH MG397173 ) ; collected by Alireza Poursafar.

Figures

FIGURE 2. Stemphylium amaranthi (IRAN 2601C). a–b. colonies on PDA (a) and PCA (b) after seven days; c–f. conidiophores and conidia; g. mature ascocarp; h–i. asci; k–l, ascospores.