Colletotrichum cariniferi X.Y. Ma, K.D. Hyde & Jayawardena

Ma, Xiaoya, Nontachaiyapoom, Sureeporn, Jayawardena, Ruvishika S., yde, Kevin D., Gentekaki, Eleni, Zhou, Sixuan, Qian, Yixin, Wen, Tingchi & Kang, Jichuan, 2018, Endophytic Colletotrichum species from Dendrobium spp. in China and Northern Thailand, MycoKeys 43, pp. 23-57 : 29

Colletotrichum cariniferi X.Y. Ma, K.D. Hyde & Jayawardena sp.nov.

Etymology.

In reference to the host epithet cariniferum.

Holotype.

MFLC 17-1199 (ex-holotype culture: MFLUCC 14-0100).

Description.

Sexual morph not observed.

Asexual morph on CMA. Vegetative hyphae 3.5-8.2 µm diam (N=20), hyaline to brown, smooth-walled, septate, branched. Appressoria (9.7-) 10.4-17 (-20.5) × (6.5-) 7.1-11.3 (-13.6) µm (N=6), globose to sub-globose, light brown. Conidiomata 50 × 50 µm (N=10), developing with mycelia, globose to irregular, milk orange to orange, in mass brown. Conidiophores (37.5-) 42.3-65 (-71.6) × (3.1-) 3.8-5.9 (-6) µm (N=6), smooth-walled, unbranched, hyaline. The part connected with conidia of conidiogenous cell inflated and some with large guttules. Conidia (24.1-) 26.8-33.0 (-36.1) × (7.9-) 8.3-9.6 (-10.2) µm (N=30), L/W = 3.4, ellipsoidal to cylindrical, with one end inflated when immature state, both ends rounded when mature, with 2 to 3 guttules, hyaline.

Cultures on CMA flat with entire margin. Growth rate: 0.23cm/day, with 50-days for sporulation. Cottony, pale cinnamon to light brown, scattered pale mycelia in spots around the middle inoculum clump, sometimes covered short, floccose-felty, white, aerial mycelium, reverse buff brown.

Material examined.

Thailand, Chiang Rai, Wat Phra That Doi Tung (Temple of Doi Tung Pagodas), the host Dendrobium cariniferum was sampled on 19 December 2013, Collector: Sureeporn Nontachaiyapoom, Natdanai Aewsakul, Xiaoya Ma.

Notes.

Colletotrichum cariniferi (MFLUCC 14-0100) clusters in a separate branch with a good support (88ML/1.00BI/59MP). The species is most phylogenetically close to Colletotrichum excelsum-altitudum and C. tropicicola , but they are morphologically different. C. cariniferi holds 77 and 91 different base pairs compared with C. tropicicola and C. excelsum-altitudum respectively. Colletotrichum cariniferi has much larger conidia than that of closely related strains in the tree (conidia (24.1-) 26.8-33 (-36.1) × (7.9-) 8.3-9.6 (-10.2) µm (N=30), L/W = 3.4 vs. conidia of C. tropicicola 13 –16.5×5– 7 μm and C. excelsum-altitudum 14.8 ± 0.8 × 5.8 ± 0.4 μm) (Noireung 2012; Tao et al. 2013). Blastn searches with sequence of MFLUCC 14-0100 resulted in 100% matches with ITS sequence of endophytic Colletotrichum sp. strain S4 isolated from Dendrobuim nobile in China (GenBank FJ042517, Yuan et al. 2009) and 96% identity with TUB2 sequences of C. arxii strain CBS 169.59 isolated from Oncidium excavatum (GenBank KF687868, Liu et al. 2014) in Netherlands and another C. arxii strain CBS 132511 isolated from Paphlopedilum sp. in Germany (GenBank KF687881, Liu et al. 2014) respectively. Colletotrichum cariniferi from stems of D. cariniferum is introduced as a new species.