Neopestalotiopsis samarangensis (Maharachch. & K.D. Hyde)

He, Yu-Ke, Yang, Qi, Sun, Ya-Ru, Zeng, Xiang-Yu, Jayawardena, Ruvishika S., Hyde, Kevin D. & Wang, Yong, 2022, Additions to Neopestalotiopsis (Amphisphaeriales, Sporocadaceae) fungi: two new species and one new host record from China, Biodiversity Data Journal 10, pp. 90709-90709 : 90709

Neopestalotiopsis samarangensis (Maharachch. & K.D. Hyde)

Neopestalotiopsis samarangensis (Maharachch. & K.D. Hyde) Maharachch., K.D. Hyde & Crous in Maharachchikumbura, Hyde, Groenewald, Xu & Crous, Stud. Mycol. 79: 147 (2014)

Materials

Type status: Other material. Occurrence: recordedBy: Yu-ke He; occurrenceID: GUCC 21003; Taxon : scientificName: Neopestalotiopsis samarangensis; order: Amphisphaeriales ; family: Sporocadaceae ; genus: Neopestalotiopsis ; Location : country: China; stateProvince: Hainan; locality: Haikou City , Xinglong Tropical Botanical Garden ; verbatimCoordinates: 110°11' E, 18°44' N; Identification: identifiedBy: Yu-ke He; dateIdentified: 2020; Record Level: collectionID: HGUP 10003

Description

Associated with leaf spots of Salacca zalacca (Gaertn.) Voss. Disease symptom: a small oval scab, ring-like, the inner ring is light brown to dark brown and the outer ring is light brown, the boundary is obvious, dark brown. A few black, small, isolated and punctuate conidia irregularly distributed on the scab. Sexual state: unknown. Asexual morph (Fig. 4 ): Conidiomata is dark, oblate, scattered on the host scab, 70-180 μm. Conidiophores discrete to lageniform or globular, hyaline, smooth- and thin-walled, simple and short. Conidia 18-23 × 6-7.5 μm, fusiform to clavate, straight to slightly, 4-septate; basal cell obconic with a truncate base, hyaline or pale brown, smooth- and thin-walled, 3.5-5 μm long; three median cells 12.5-15 μm long, light brown or hyaline, septa and periclinal walls darker than rest of the cell, wall rugose; second cell 4.5-5.5 μm long; third cell 4-5.5 μm long; fourth cell 5-6 μm long; apical cell 3-4.5 μm long, hyaline, conic to acute, with 1-2 tubular appendages inserted at different loci, but in the same crest at the apex of the apical cell, unbranched, flexuous, 12-20 μm long. The spores have tubular appendages or single appendage, unbranched, centric, 3.5-6 μm long.

Culture characteristics: Colonies on PDA medium reaching 4.5-5 cm diam. After 9 d at 24℃, odourless, without exudates, with black dots in the centre (conidiomata), the mycelium is white, soft and round with regular edges; reverse yellow to white. Under the surface of hyphal layer, releasing many conidia in a black, slimy mass.

Notes

Phylogenetically, isolated GUCC 21003 clustered with the ex-type strain of N. samarangensis (MFLUCC 12-0233). In morphology, our strain is very similar to N. samarangensis ( Maharachchikumbura et al. 2013). A comparison of DNA bases (Table 2 ) demonstrated that the differences between these two strains are minute. Therefore, we concluded that they are the same species, but occurring on different hosts ( N. samarangensis GUCC 21003 on leaf of Salacca zalacca vs. N. samarangensis MFLUCC 12-0233 on Syzygium samarangense ).

Figures

Figure 4. Neopestalotiopsis samarangensis (Specimen code: HGUP 10003). a-b Appearance on host surface; c Colony top view and reverse view; d-e Conidiomata on PDA; f Conidiogenous cells; g-j Conidia. Scale bars: a = 10 mm, b = 1 mm, d-e = 500 μm, f-j = 20 μm.