Labrundinia neopilosella Beck and Beck, 1966

Silva, Fabio Laurindo Da, Fonseca-Gessner, Alaide Aparecida & Ekrem, Torbjørn, 2014, A taxonomic revision of genus Labrundinia Fittkau, 1962 (Diptera: Chironomidae: Tanypodinae), Zootaxa 3769 (1), pp. 1-185 : 89-94

publication ID	https://doi.org/ 10.11646/zootaxa.3769.1.1
publication LSID	lsid:zoobank.org:pub:705567C6-C912-4A19-9DE2-726254E17076
DOI	https://doi.org/10.5281/zenodo.5621067
persistent identifier	https://treatment.plazi.org/id/676987A8-B75A-FF8C-FF29-FF6CFB9DF95F
treatment provided by	Plazi
scientific name	Labrundinia neopilosella Beck and Beck, 1966
status

Treatment

Labrundinia neopilosella Beck and Beck, 1966 View in CoL

( Figs 42–44 View FIGURES 42 A – M View FIGURES 43 A – E View FIGURES 44 A – I )

Labrundinia neopilosella Beck and Beck, 1966: 341 View in CoL (description of adults and immatures); Roback 1971: 278 (description of male); Roback 1987: 193 (description of immatures).

Material examined. Type material: Holotype male with larval exuviae, USA: Florida, Flagler County, Rayonier Ditch, 3.vi.1964, W. Beck ( USNM 69125). Paratype: 1 male with pupal and larval exuviae same data as holotype except for 27.ii.1965.

Additional material: 3 larvae, USA: North Carolina, Forsyth, Belews Lake, 16.ix.1985, W. Beck. 1 male with pupal exuviae, South Carolina, Pickens, Clemson University, 16.ix.1985, P. L. Hudson; 1 larva as previous except for Anderson, Fish Pond, 25.iii.1977, P. H. Carlson and T. R. White.

Diagnostic characters. Labrundinia neopilosella differs from other Labrundinia species by the combination of the following Characters. Adult male: abdominal tergite I pale brown, T II–IV with continuous brown transverse band near proximal margin, T V–VIII completely brown; hypopygium pale brown, sternapodeme with reduced anterior process. Pupa: thoracic horn ovoid; preapical indentation absent; thoracic horn about 2.5–2.9 times as long as wide. Larva: Surface of head capsule smooth, lateroventral spine group present with several conspicuous spines; subbasal seta of posterior parapod serrated; bifid claw with U-shaped lower indentation.

Description. Adult male (n = 4 unless otherwise stated)

Size. Total length 1.6–2.0 (3) mm. Wing length 1.0– 1.2 mm. Total length/wing length 1.33–1.75 (3). Wing length/ profemur length 2.80–2.85 (3).

Coloration. Head pale brown with dark brown occipital margin; pedicel and antenna brown; maxillary palp pale brown. Thorax brown with dark scutum; antepronotum pale brown; supraalar callus pale brown. Wing membrane transparent without any marking. Legs pale. Femur I–III pale. Tibia I–II pale, Ti III pale brown. Tarsomere 1–5 pale to pale brown. Abdominal tergite I pale brown, II–IV with continuous brown transverse band near proximal margin, T V–VIII completely brown; hypopygium pale brown ( Fig. 42L View FIGURES 42 A – M ).

Head ( Figs 42A–E View FIGURES 42 A – M ). Temporal setae 10–14 (3), uniserial ( Fig. 42B View FIGURES 42 A – M ). Eye ratio 0.84–0.96 (2). Tentorium ( Fig. 42C View FIGURES 42 A – M ) 98–128 (2) Μm long. Clypeus 58–92 (2) Μm long, 53–63 (2) Μm wide at largest part, bearing 12–17 setae. Cibarial pump 144–165 (3) Μm long ( Fig. 42D View FIGURES 42 A – M ), with anterior margin concave. Lengths of palpomeres 1–4 (in Μm): 23–29 (3); 41–47 (3); 88–92 (3); 90–120 (2); palpomeres 5 broken off. Antennal flagellum 582–678 (3) Μm long, diameter of pedicel 96–108 Μm, apical setae single ( Fig. 42E View FIGURES 42 A – M ), AR 1.15–1.19 (3).

Thorax. Antepronotum with 3–5 (2) lateral setae. Acrostichals 30–36 (2), biserial, diverging evenly posteriorly, starting close to antepronotum and almost reaching scutellum; dorsocentrals 18–26, irregularly uniserial; prealars 7–10; supraalars 3. Anapleural suture ratio 0.35–0.50 (2). Scutellum with transverse row of 8–10 (3) setae and group of numerous fine anterior setae.

Wing ( Fig. 42F View FIGURES 42 A – M ). Width 0.3 mm. Costa 0.8–1.0 mm long. R2+3 absent. VR 0.64–0.71. WW 0.26–0.30. Brachiolum with 2 setae. Squama setiferous.

Legs ( Figs 42G–K View FIGURES 42 A – M ). Fore leg: width at apex of tibia 32–39 Μm ( Fig. 42G View FIGURES 42 A – M ), tibia with single, apical and pectinate spur 15–16 (3) Μm long ( Fig. 42H View FIGURES 42 A – M ), with three lateral teeth and two preapical setae; ta1-4 without any preapical pseudospurs. Mid leg: width at apex of tibia 31–39 Μm long ( Fig. 42I View FIGURES 42 A – M ), tibia with single, apical and pectinate spur 17–18 (3) Μm long with three lateral teeth ( Fig. 42J View FIGURES 42 A – M ) and two preapical setae; ta1-4 each with two preapical pseudospurs. Hind leg: width at apex of tibia 29–39 Μm long ( Fig. 42K View FIGURES 42 A – M ), tibia without spur; comb with 5 (2) bristles; ta1-4 without any preapical pseudospurs. Claws slender, hooked; pulvilli absent. Lengths and proportion of leg segments as in Table 27.

Hypopygium ( Fig. 42M View FIGURES 42 A – M ). Tergite IX arched, with 10–14 posterior setae. Membranous anal point rounded, notched apically. Phallapodeme 39–49 (3) Μm long. Sternapodeme with reduced anterior process. Gonocoxite cylindrical, 84–96 Μm long, 41–46 Μm wide, with slightly concave inner margin. GcR 1.86–2.23. Gonostylus simple and slender, 58–63 Μm long; megaseta 9–12 (3) Μm long. HR 1.36–1.53. HV 2.64–3.10 (3).

Adult female (n = 1)

Size. Total length 0.8 mm. Wing length 0.9 mm. Total length/wing length 0.98. Wing length/ profemur length 2.68.

Coloration. Head, pedicel, antenna and maxillary palp pale brown. Thorax pale brown with dark scutum. Wing membrane transparent without any marking. Legs pale. Abdomen completely pale brown, not very discernible. Seminal capsules brown.

fe ti ta1 ta2 ta3

p1 414–453 452–484(1) 254–274 207–218 133–145 p2 487–523 352–416 453–507 180–242 133–141 p3 469–477 484–586 460–500 219–242 148–179

ta4 ta5 LR BV SV

p1 103–109 62–63 0.60–0.63 2.26–2.38 2.95–3.34 p2 101–102 63–70 1.30–1.38 2.68–2.69 1.68–1.74 p3 101–109 63–78 0.95–098 2.45–2.48 1.86–1.95 Head. Temporal setae 10, uniserial. Eyes ratio 1.04. Tentorium 107 Μm long. Clypeus 74 Μm long, 71 Μm wide at largest part, bearing 19 setae. Cibarial pump 142 Μm long, as in male. Lengths of palpomeres 1–5 lengths (in Μm): 34; 52; 65; 92; 147. Antennal flagellum 299 Μm long, diameter of pedicel 49 Μm, AR 0.42.

Thorax. Antepronotum with 6 lateral setae. Acrostichals 36, irregularly biserial, starting close to antepronotum; dorsocentrals 32, irregularly uniserial; prealars 11; supraalars 3. Scutellum with transverse row of 9 setae and group of numerous fine anterior setae.

Wing. Width 0.3 mm. Costa 0.7 mm long. R2+3 absent. VR 0.82. WW 0.35. Brachiolum with 2 setae. Squama setiferous.

Legs. Fore leg: width at apex of tibia 36 Μm, tibia with single, apical and pectinate spur 14 Μm long, with three lateral teeth and two preapical setae; preapical pseudospurs on ta1-4 not observed. Mid leg: width at apex of tibia 28 Μm, tibia with single, apical and pectinate spur 18 Μm long, with three lateral teeth and two preapical setae; preapical pseudospurs on ta1-4 not observed. Hind leg: width at apex of tibia 27 Μm, tibia without spur; comb setae not observed; preapical pseudospurs on ta1-4 not observed. Claws slender, hooked; pulvilli absent. Lengths and proportion of leg segments as in Table 28.

fe ti ta1 ta2 ta3 p1 334 313 242 141 107 p2 470 361 – – –

p3 417 484 441 241 100

ta4 ta5 LR BV SV p1 85 62 0.77 2.25 2.67 p2 – – – – –

p3 99 77 0. 91 2.59 2.04 Genitalia. Gonapophysis VIII broadly rounded, 67 Μm long. Coxosternapodeme 108 Μm long. Postgenital plate rounded. Cerci oval-quadrate, 38 Μm long and 21 Μm wide; with 4 elongated setae. Labia with inconspicuous microtrichia. Notum length 108 Μm long. Seminal capsules oval, 50 Μm long, 30 Μm wide, with conical shaped necks. Length ratio SCa/No 0.47.

Pupa (n = 4 unless otherwise stated)

Size. Abdomen1.7–1.8 (3) mm long in male, 1.8 (1) mm long in female.

Coloration. Exuviae mostly pale brown; thoracic horn and apex of anal lobe brown.

Cephalothorax ( Figs 43A–C View FIGURES 43 A – E ). Frontal apotome smooth ( Fig. 43A View FIGURES 43 A – E ). Wing sheath smooth 0.7–0.8 (3) mm long. Thoracic horn 219–231 Μm long and 76–101 Μm wide ( Fig. 43B View FIGURES 43 A – E ), THR 2.54–2.91, preapical indentation absent. Membranous preapical papilla 22–37 Μm long ( Fig. 43C View FIGURES 43 A – E ), PTH 0.10–0.17, aeropyle tube simple, elongated, 28–29 (2) Μm long; plastron plate small. Horn sac well developed, completely filling respiratory atrium. Reticulation of respiratory atrium indistinct. Basal lobe wedge-shaped. Thoracic comb with 8–10 conical tubercles ( Fig. 43B View FIGURES 43 A – E ).

Abdomen ( Figs 43D–E View FIGURES 43 A – E ). Tergite I with scar 114–120 (2) Μm long. T I–VI without shagreen, T VII–VIII with shagreen basally concentrated. Abdominal chaetotaxy as in figure 43D. Abdominal segment VII with 4 LS-setae. A VIII with 5 LS-setae. Anal lobe 234–258 (3) Μm long and 148–171 Μm wide ( Fig. 43E View FIGURES 43 A – E ), outer margins sclerotized, with 8 (2) spines, longest spine 14–16 (3) Μm long, inner margins of lobes membranous. ALR 1.58–1.64 (3). Male genital sac extending somewhat beyond apex of anal lobe.

4th instar larva (n = 8 unless otherwise stated)

Coloration. Head pale yellow; postoccipital margin brown. Second antennal segment brown; distal tooth of mandible and apex of ligula brown. Abdomen pale yellow; procercus and anal setae pale brown. Posterior parapod claws all pale yellow.

Head ( Fig. 44A View FIGURES 44 A – I ). Length 438–463 (4) Μm, 254–297 (4) Μm wide. IC 0.56–0.75. Surface smooth; lateroventral spine group present, well developed, with 7–10 (7) spines; posteroventral spine group absent. Ventral cephalic setae S9, S10 and SSm forming a more or less straight line diagonal to longitudinal axis of head capsule ( Fig. 44A View FIGURES 44 A – I ).

Antenna ( Figs 44B–C View FIGURES 44 A – I ). Length 246–288 Μm, A1 148–189 Μm long, with ring organ located 0.77–0.86 from base, A2 68–78 Μm long. AR 1.85–2.13 (7). Blade longer than A2 over-reached by accessory blade.

Maxilla ( Fig. 44D View FIGURES 44 A – I ). Basal palp segment 24–38 Μm long and 8–18 Μm wide at the middle, with ring organ 0.76– 0.91 (6) from base. PR 2.14–3.20 (3). APR 4.80–7.60.

Mandible ( Fig. 44E View FIGURES 44 A – I ). Length 60–73 Μm. Sensillum campaniformium located 0.68–0.85 from apex, basal tooth bifid, with seta subdentalis projecting from sloping end towards apical tooth, accessory tooth present, AMD 2.28– 3.05.

Mentum and M appendage . Dorsomental teeth reduced; pseudoradula uniformly granulate.

Hypopharyngeal complex ( Figs 44F–G View FIGURES 44 A – I ). Ligula 52–65 Μm long, 24–27 Μm wide, with row of 5 teeth. IO 0.95– 0.99 (7), MO 1.08–1.15. Paraligula bifid, 20–29 Μm long, inner tooth 19–26 (7) Μm long, shorter than outer tooth. Pecten hypopharyngis with 8 teeth almost equal in size.

Body ( Figs 44H–I View FIGURES 44 A – I ). Without lateral fringe. Anterior parapods with simple claws. Procercus 125–151 (6) Μm long, 22–40 Μm wide, with 7 anal setae 494–650 (5) Μm long. L/ W 4.60 –6.00 (5). Supraanal seta well developed. Anal tubules 173–210 (4) Μm long. Posterior parapod 251–251 (4) Μm long; subbasal seta on posterior parapod serrated, with 5–9 (7) spines basally ( Fig. 44H View FIGURES 44 A – I ); parapod apex with numerous simple claws; bifid claw with Ushaped lower indentation ( Fig. 44I View FIGURES 44 A – I ). B/C 0.88–0.96 (7).

Remarks. Adult males of Labrundinia neopilosella appear most similar to those of L. setosa in the abdominal coloration. However, the completely brown abdominal tergite VI and the pale brown hypopygium present in L. neopilosella contrast the anteriorly brown abdominal tergite VI (anterior ¾) and the dark brown hypopygium in L. setosa . The pupae of L. neopilosella appear most similar to those of L. becki but can be distinguished from this species by the insertion of aeropyle tube into the upper margin of the preapical indentation in the thoracic horn. The larvae of L. neopilosella resemble those of L. becki and L. longipalpis , but may be separated from the former by the basally serrated subbasal seta and the bifid claw with U-shaped lower indentation of the posterior parapod. The subequal inner and outer teeth of the ligula and the pecten hypopharyngis with 7 teeth distinguish larvae of L. neopilosella from those of L. longipalpis . Labrundinia neopilosella is widespread in the United Stated (Roback 1987). The larvae were collected in in small lakes, creeks and unpolluted pools.