Ngirhaphium sivasothii Grootaert & Puniamoorthy, 2014

Samoh, Abdulloh, Satasook, Chutamas & Grootaert, Patrick, 2019, NGS-barcodes, haplotype networks combined to external morphology help to identify new species in the mangrove genus Ngirhaphium Evenhuis & Grootaert, 2002 (Diptera: Dolichopodidae: Rhaphiinae) in Southeast Asia, Raffles Bulletin of Zoology 67, pp. 640-659 : 651-653

Ngirhaphium sivasothii Grootaert & Puniamoorthy, 2014

( Figs. 11 , 12A )

Ngirhaphium sivasothii Grootaert & Puniamoorthy, 2014: 150 (figs. 6–8, 9, 10, 17). Type locality: SINGAPORE: Semakau Island .

Diagnosis. A medium-sized species (4.5–5.5 mm), generally with dark infuscate wing and with longitudinal veins and Tp (posterior cross vein) brownish seamed. Mesonotum and tergites metallic green. Apical aristal segment shorter, but nearly as long as basal segment. Male with dorsal surstylus half as long as cerci, with a rectangular bend, set with very long bristles ( Fig. 12A ). Cercus much longer than dorsal surstylus, tip wide, rounded, set with many long yellow bristles ( Fig. 12A detail). Outer prong of apical fork on the fore tarsomere 4 slightly shorter than inner prong.

Material examined. THAILAND: 2 males, 1 female; Langu, Bakan Tohthid (6°47′29.7″N 99°48′53.5″E), Samoh A, sweep netting, 1 June 2015, ( PSUNHM); Krabi province : 3 males,

5 females; Muaeng, Sai Thai, Khlong Chilat (8°03′23.0″N 98°53′37.4″E); Samoh A, sweep netting, 21 February 2015 ( PSUNHM) ; 1 male, 1 female; Koh Lanta, Khlong Yang (7°48′21.2″N 99°07′36.1″E); Samoh A, sweep netting, 13 June 2015 ( PSUNHM); Trang province : 3 females; Kantang, Nakluea, Ban Haad Saikhao (7°19′31.3″N 99°28′43.9″E); Samoh A, sweep netting, 15 July 2018 ( PSUNHM); Phang Nga province : 2 males, 5 females; Thappud, Ban Khuan Bosane pier (8°26′43.8″N 98°35′40.6″E); Samoh A, sweep netting, 13 February 2015 ; 1 male, 2 females, Takuapa, Bangnaisi, Ban Bangdong (8°55′46.7″N 98°23′15.4″E), Samoh A, sweep netting, 11 February 2015 ( PSUNHM) ; 14 females, Takuapa, Bangnaisi, Ban Bangnaisi (8°56′18.4″N 98°23′43.5″E), Samoh A, sweep netting, 11 February 2015 ( PSUNHM) ; 1male, 14 females, Takuapa, Bangnaisi, Ban Bangnaisi (8°56′18.4″N 98°23′43.5″E), Samoh A, sweep netting, 12 February 2015 ( PSUNHM) ; 1 male, Muang, Ban Bang Toei (8°21′54.4″N 98°34′50.9″E), Samoh A, Malaise trap, 13 February 2015 ( PSUNHM) ; 2 males, 5 females, Takua Thung, Kalai, Ban Khuan Kalai (8°19′12.8″N 98°25′46.2″E); Samoh A, sweep netting, 14 July 2018 ( PSUNHM); Ranong Province : 1 male, 4 females; Kraburi, Lam Liang, Phra Khayang cave (10°19′25.5″N 98°45′58.0″E), Samoh A., sweep netting, 10 July 2018 ( PSUNHM) .

Singapore: 3,127 specimens recorded (1,628 specimens NGS barcoded; LKCNHM).

Distribution. Singapore and South Thailand, Andaman Sea.

Haplotype network. Number of sequences used: 35 Selected region: 1–313

Number of sites: 313

Number of Haplotypes, h: 28

Figures

Fig. 11. Haplotype network of Ngirhaphium sivasothii. Thailand: Krabi, Phangnga, Satun; Singapore: Sarimbun, Pulau Tekong, Pulau Ubin, Labrador, Semakau Island, and Sungei Buloh.

Fig. 12. Compilation of male terminalia of Ngirhaphium in lateral view. A, Ngirhaphium sivasothii left side with ventral surstylus removed; cerci dorsally; B, Ngirhaphium murphyi left side with ventral surstylus removed; cerci and dorsal surstyli dorsally; C, Ngirhaphium caeruleum left side with ventral surstylus removed; cerci and dorsal surstyli dorsally; D, Ngirhaphium meieri, new species left side with ventral surstylus removed; E, Ngirhaphium chutamasae left side with ventral surstylus removed; F, Ngirhaphium thaicum, new species right side. Scale = 0.1 mm.