Diaporthe shuichengensis C. G. Ren, 2025

Ren, Chunguang, Liu, Yu, Su, Wenwen, Han, Zhengcheng & Li, Weijie, 2025, Two new species of Diaporthe (Diaporthaceae, Diaporthales) from Actinidia chinensis in Guizhou Province, China, MycoKeys 121, pp. 291-310 : 291-310

Diaporthe shuichengensis C. G. Ren sp. nov.

Fig. 7

Diagnosis.

Diaporthe shuichengensis can be distinguished from the closely related species D. passiflorae and D. malorum based on the ITS, tef 1, tub 2, his 3, and cal loci. Diaporthe shuichengensis differs from D. passiflorae in that it possesses longer alpha conidia and from D. malorum in that it possesses wider beta conidia.

Etymology.

Referring to the locality of the holotype, Shuicheng City, Guizhou Province, China.

Description.

Conidiomata: pycnidial, globose or conical, growing on the surface of pine needles, gray to black, with white villous hyphae on the surface. Conidiophores reduce to conidiogenous cells. Conidiogenous cells: colorless, transparent, smooth, and without branches, and acuminate apex; size, 14.8–30.9 × 1.3–2.6 um (mean = 22.5 × 1.9, n = 30). Alpha conidia: transparent, elliptic, obtuse at both ends, with 2 oil droplets, no septum; size, 5.2–8.1 × 1.3–2.8 μm (mean = 6.6 × 1.9, n = 30); Beta conidia: unicellular, septate, and linear; one of their ends was straight and the other was slightly curved; size, 17.3–29.7 × 1.3–2.7 μm (mean = 23.3 × 2.0, n = 30).

Culture characteristics.

After 15 days of culture on PDA at 25 ° C under dark conditions, the colonies were white to light green in color, with the back appearing white to purple.

Holotype.

China • The Guizhou Province: Shuicheng City ( 26°25'8.65"N, 104°57'33.67"E), from kiwifruit soft rot, October 11, 2023, Chunguang Ren; ( holotype GZMHT SC-7.; ex-type living culture: SC-7; living culture: SC-8).

Notes.

The two strains of D. shuichengensis sp. nov. formed a distinct clade with high bootstrap value (100 % ML, 1 BI); they were closely related to D. passiflorae , D. malorum and D. eucommiigena . Compared with the typical characteristics of the known species (Table 3 ). D. shuichengensis sp. nov. differs from D. passiflorae and D. malorum in that it possesses larger beta conidia 17.3–29.7 × 1.3–2.7 μm vs. (14 –) 16–18 (– 20) × 1.5 (– 2) μm. and (17.4) – 21.5 – (26.6) × (0.8) – 1.3 – (2.0) μm). D. shuichengensis was distinguished from eucommiigena by its shorter beta conidia (17.3–29.7 × 1.3–2.7 μm vs. 27–37 × 1–2 μm). Thus, the morphological characteristics and molecular phylogenetic results support D. shuichengensis as a new species.

Figures

Figure 7. Diaporthe shuichengensis sp. nov. (SC- 7). A. Upper view of the colony; B. Reverse view of the colony; C. Conidiomata; D – E. Conidiogenous cells; F – H. Alpha- and beta-conidia. Scale bars: 10 μm (D – E); 5 μm (F – H).