Blastocystis, Jaekel, 1918

Rondon ́, Silvia, Ortiz, Mario, Leon ́, Cielo, Galvis, Nelson, Andres ́ Link & Gonzalez, Camila, 2017, Seasonality, richness and prevalence of intestinal parasites of three neotropical primates (Alouatta seniculus, Ateles hybridus and Cebus versicolor) in a fragmented forest in Colombia, International Journal for Parasitology: Parasites and Wildlife 6 (3), pp. 202-208 : 204

2.4. Molecular analyses for Blastocystis and Giardia

To perform detection of Blastocystis hominis and Giardia sp. , we extracted DNA from faecal samples stored in ethanol using a QIAamp DNA Stool Kit (Qiagen), and its QIAamp DNA Stool Handbook Protocol. DNA concentration was measured for all extracts using a nanodrop device. Blastocystis hominis detection was performed through PCR using the primers RD5 and BhRDr ( Scicluna et al., 2006) and Go Taq Master Mix, targeting a 607bp fragment. Additionally, a semi-nested PCR assay was performed for Giardia sp. detection, using the primers GDHeF, GDHiF and GDHiR ( Read et al., 2004). Reactions were performed using Go Taq Master Mix targeting a 450 — 500 pb fragment.

All PCR products were visualized on agarose gel and positive samples were sequenced. Sequences were analysed using Geneious Software and compared with publicly available sequences using BLAST (National Center for Biotechnology Information).