Neospora caninum

Adri, Hern, an, andez-Ortiz, Emilie, Bouchard, Snyman, Louwrens P., Al-Adhami, Batol H., Gouin, eraldine-G., Neelin, Mikhaela & Jenkins, Emily J., 2023, Toxoplasma gondii and related Sarcocystidae parasites in harvested caribou from Nunavik, Canada, International Journal for Parasitology: Parasites and Wildlife 21, pp. 246-254 : 248

2.3.2. Competitive ELISA for Neospora caninum

The commercially available competitive ELISA kit ( Neospora caninum antibody test kit, cELISA, VMRD, WA, USA) was performed following the manufacturer’ s protocol. Fifty μL/well per sample, including kit controls, were added directly to the plate in duplicate. Results were interpreted by calculating the percentage of inhibition (%I) using the kit controls. Results were classified as negative when the samples were <30 %I, and positive if the samples were> 30 %I.