Claviradulomyces machaeriae D.M.Macedo & R.W.Barreto, 2021

Duarte, Lidiane Leal, M, Davi, Macedo, esquita de & Barreto, Robert Weingart, 2021, Cryptic but ubiquitous: Claviradulomyceae fam. nov. with five novel species of the lenticel fungus Claviradulomyces from Brazil, Cryptogamie, Mycologie 20 (7), pp. 121-135 : 125-129

Claviradulomyces machaeriae D.M.Macedo & R.W.Barreto , sp. nov.

( Fig. 3 )

MYCOBANK NUMBER. — MB 838369.

HOLOTYPE. — Brazil. State of Rio de Janeiro, Nova Friburgo, Riograndina, on branches of Machaerium sp. ( Fabaceae ), 7.IV.2014, R. W.Barreto ( VIC 42840; culture COAD 1936; GenBank LSU sequence KP 975453).

ETYMOLOGY. — Refers to its host-genus Machaerium .

DIAGNOSIS. — Similar to C. xylopiae but having a pale brown to olivaceous pycnidial body instead of dark brown as in C. xylopiae .

HABITAT AND DISTRIBUTION. — Inside lenticels on branches of X. sericea in a secondary Atlantic Forest and on lenticels on branches of Machaerium sp. in ruderal (roadside) situation.

DESCRIPTION

Asexual morph on living branches bearing abnormal hypertrophied lenticels with spongy tissue. Conidiomata pycnidial, commonly several per lenticel, subglobose, 50-95 Μm diam, basal narrow layer of subhyaline, walls subhyaline to olivaceous, textura intrincata at base, changing into pale brown textura angularis in upper half, smooth, ostiolate, rostrate: neck long, 140-340 × 20-37 Μm, slightly curved, dark brown to olivaceous becoming subhyaline at the tip, of textura porrecta. Conidiophores reduced to conidiogenous cells, holoblastic, suboblate to somewhat lageniform, straight, 5-8 × 1.5-2 Μm, hyaline, smooth. Conidia fusoid-filiform, 25-35 × 2-3 Μm, straight to slightly curved, attenuated toward the acute tip, aseptate, guttulate, subhyaline to hyaline, smooth. Sexual morph not observed.

Culture characteristics

Very slow-growing (1.5-2.5 cm diam after 40 days). Colonies dry or moist, flat to slightly raised centrally, margins even or lobate, aerial mycelium scarce, mostly dark brick with cinnamon edges or ochreous centrally followed by a dark mouse grey band, ending with a narrow honey periphery or velvet isabelline mycelium centrally and powdery buff mycelium at the periphery; not sporulating.

NOTES

Claviradulomyces machaeriae D.M.Macedo & R.W.Barreto , sp. nov. is morphologically similar to C. xylopiae, However , the color of the pycnidial body is olivaceous in C. machaeriae D.M.Macedo & R.W.Barreto , sp. nov. and dark brown in C. xylopiae . The neck is bigger in C. machaeriae D.M.Macedo & R.W.Barreto , sp. nov. in comparison with C. xylopiae . The combination of morphological and molecular, using rpb 2 confirm the fungus on Machaerium as a distinct species, although close to C. xylopiae .

Figures

FIG. 3. — Claviradulomyces machaeriae D.M.Macedo & R.W.Barreto, sp. nov.: A, branch of Machaerium sp. showing abnormal lenticels colonized by C. machaeriae sp. nov. (VIC 42840); B, C, pycnidia on lenticels.D, E, pycnidia; F, group of immature conidia attached to the conidiogenous cells; G, conidium. Scale bars: B-E 20 µm; F, G, 10 µm.