Neopestalotiopsis zingiberis Y.K. He & Yong Wang bis, 2022

He, Yu-Ke, Yang, Qi, Sun, Ya-Ru, Zeng, Xiang-Yu, Jayawardena, Ruvishika S., Hyde, Kevin D. & Wang, Yong, 2022, Additions to Neopestalotiopsis (Amphisphaeriales, Sporocadaceae) fungi: two new species and one new host record from China, Biodiversity Data Journal 10, pp. 90709-90709 : 90709

Neopestalotiopsis zingiberis Y.K. He & Yong Wang bis sp. nov.

Materials

Type status: Holotype. Occurrence: recordedBy: Yu-ke He; occurrenceID: GUCC 21001; Taxon : scientificName: Neopestalotiopsis zingiberis; order: Amphisphaeriales ; family: Sporocadaceae ; genus: Neopestalotiopsis ; Location : country: China; stateProvince: Hainan; locality: Haikou City , Wuzhishan Nature Reserve ; verbatimCoordinates: 109°32' E, 18°48' N; Identification: identifiedBy: Yu-ke He; dateIdentified: 2020; Record Level: collectionID: HGUP 10001

Description

Associated with leaf blight of Zingiber officinale Rosc. Disease symptom: A long oval to irregular, ring-like scab, light brown, edge reddish-brown, slightly sunken on adaxial surface. The boundary of the scab is obvious, with a narrow yellow halo around the scab. There are many black, small and punctuate conidia on the scab. Sexual state: unknown. Asexual morph (Fig. 3 ): Conidiomata is dark, oblate, scattered on the host scab, 104-202 μm. Conidiophores discrete to lageniform, hyaline, smooth- and thin-walled, annellidicae, 12-25 × 3-6 μm (n = 40). Conidia 21-31 × 6-9.5 μm, fusiform to clavate, straight to slightly, 4-septate; basal cell obconic with a truncate base, hyaline or pale brown, smooth- and thin-walled, 3-6 μm long; three median cells 15-19 μm long, septa and periclinal walls darker than rest of the cell, versicoloured, wall rugose; second cell brown, 4-6 μm long; third cell brown, 4-7 μm long; fourth cell light brown 4-6 μm long; apical cell 3-5 μm long, hyaline, conic to acute, with 1-3 tubular appendages insert at different loci, but in the same crest at the apex of the apical cell, unbranched, flexuous, 12-15 μm long; most spores have no tubular appendages or single appendage, unbranched, centric, 0-6 μm long.

Culture characteristics: Colonies on PDA medium reaching 8-9 cm diam. after 15 d at 24℃, the mycelium is yellowish or white, soft and round with irregular edges. Under the surface of hyphal layer, releasing conidia in a black, slimy mass. Dark brown pigment is deposited on the bottom of the Petri dish.

Etymology

Neopestalotiopsis zingiberis , in reference to the host genus ( Zingiber ) from which it was isolated.

Notes

Neopestalotiopsis zingiberis (GUCC 21001) formed a distinct clade and sistered to Neopestalotiopsis magna (MFLUCC 12-0652) (Fig. 1 ). Morphologically, conidia of N. zingiberis (21-31 × 6-9.5 μm) are smaller than N. magna (42-46 × 9.5-12 μm) and also differed by having branched, flexuous apical tubular appendages ( Maharachchikumbura et al. 2014a) (Table 3 ). Thus, we propose N. zingiberis as a novel taxon.

Figures

Figure 1. Consensus phylogram of 1,000 trees resulting from an RAxML analysis of the (ITS + TUB 2 + TEF 1) alignment of the analysed Neopestalotiopsis sequences. Pestalotiopsis diversiseta (MFLUCC 12 - 0287) is used as the outgroup taxon. The MP bootstrap values ≥ 50 %, ML bootstraps ≥ 70 % and Bayesian posterior probabilities ≥ 0.90 (MPBS / MLBS / PPBY) are given at the nodes. New collections obtained in this study are in red.

Figure 3. Neopestalotiopsis zingiberis (Specimen code: HGUP 10001). a-b Appearance on host surface; c Colony top view and reverse view; d-e Mycelium; f Conidiogenous cells; g-i Conidia. Scale bars: a = 10 mm, b = 1 mm, d-e = 200 μm, f-i = 20 μm.