Zeugodacus (Sinodacus) madhupuri Leblanc & Doorenweerd

Leblanc, Luc, Hossain, M. Aftab, Doorenweerd, Camiel, Ahmed Khan, Shakil, Momen, Mahfuza, San Jose, Michael & Rubinoff, Daniel, 2019, Six years of fruit fly surveys in Bangladesh: a new species, 33 new country records and discovery of the highly invasive Bactrocera carambolae (Diptera, Tephritidae), ZooKeys 876, pp. 87-109 : 87

Zeugodacus (Sinodacus) madhupuri Leblanc & Doorenweerd sp. nov. Figs 6 A–D ; 7 A–C

Holotype.

Male. Labelled: "Bangladesh, Tangail District, Madhupur National Park, 24.702375N, 90.086325E, 5-13-ix-2018, M. Aftab Hossain, FFBn-316, cue-lure", labelled as molecular voucher ms08804. Deposited at UHIM. Paratypes: One male. Labelled: "Bangladesh, Tangail District, Madhupur National Park, 24.704048N, 90.077770E, 5-13-ix-2018, M. Aftab Hossain, FFBn-311, cue-lure". Deposited at WFBM. Two males labelled: "Bangladesh, Tangail District, Madhupur National Park, 24.703023N, 90.078774E, 5-13-ix-2018, M. Aftab Hossain, FFBn-312, cue-lure", labelled as molecular vouchers ms08805 and ms08806. Deposited at UHIM.

Differential diagnosis.

Zeugodacus madhupuri is similar to the Indian Zeugodacus (Sinodacus) brevipunctatus (David & Hancock) ( David et al. 2017), but differs in that the fuscous medial band and lateral markings on the abdomen are pale and less extensive than in Z. brevipunctatus , dark marking on legs are fulvous rather than fuscous, and Z. madhupuri consistently has two pairs of equally well-developed scutellar setae. Zeugodacus brevipunctatus , along with most other species of subgenus Sinodacus Zia has only one pair of scutellar setae ( Hancock and Drew 2018a).

Molecular diagnostics.

We obtained COI-3P sequences for three specimens, aligned them with the available COI-3P sequences from San Jose et al. (2018a) and performed maximum likelihood analyses. The full tree is available in Supplementary material 2: Figure S2, and a subset of Z. madhupuri and its closest relatives is shown in Figure 5 . Based on our reference dataset, the new species is most similar to Z. hengsawadae (Drew & Romig) and Z. heinrichi (Hering) at around -11% pairwise distance and can be diagnosed reliably using COI. Note however that Zeugodacus brevipunctatus was not represented in our COI dataset.

Description of adult.

Head ( Fig. 6A, B ). Vertical length 1.65-1.95 mm. Frons, of even width, 1.11-1.23 times as long as broad; fulvous with anteromedial hump covered by short red-brown hairs; orbital setae large-sized and dark fuscous and strong: one pair of superior and three pairs of inferior fronto-orbital setae present, the most anterior pairs nearly contiguous; lunule yellow. Ocellar triangle black. Vertex fuscous. Face fulvous to yellow with a broad transverse black band at mid height; length 0.58-0.75 mm. Genae fulvous, with or without a faint fuscous subocular spot; red-brown seta present. Occiput fulvous and yellow along eye margins; with two pairs of large occipital dorsal setae and lateral occipital rows with 5-8 light to dark setae. Antennae with scape, pedicel and first flagellomere fulvous and arista black (fulvous basally); length of segments: 0.20-0.25 mm; 0.25-0.35 mm; 0.83-0.93 mm.

Thorax ( Fig. 6B ). Scutum fulvous with very narrow median and lateral faint fuscous longitudinal lines. Pleural areas fulvous except well-defined or faint red-brown area narrowly along anterior margin of mesopleural stripe, over most of anepimeron, and above mid coxae. Yellow markings as follows: postpronotal lobes (narrowly fulvous anteriorly); notopleura (notopleural callus); broad mesopleural (anepisternal) stripe, reaching level of anterior notopleural seta dorsally, continuing to katepisternum as a broad transverse spot, anterior margin straight or slightly convex; anatergite (with or without posterior margin narrowly red-brown); anterior 75% of katatergite (remainder fulvous); a narrow medial postsutural vitta and two narrow lateral postsutural vittae tapering posteriorly ending before or faintly reaching intra-alar setae, and prolonged narrowly anteriorly beyond notopleural suture. Postnotum fulvous. Scutellum yellow except for very narrow black basal band. Setae: four scutellar (both pairs well developed); prescutellar absent; one intraalar; one posterior supraalar; anterior supraalar absent; one mesopleural; two notopleural; four scapular; all setae well developed and red-brown. A weakly to well-developed postpronotal seta present in some specimens.

Legs ( Fig. 7B ). Fore coxae basally and posteriorly dark fuscous and anteroapically fulvous. Mid and hind coxae predominantly dark fuscous. Trochanters fulvous. Femora fulvous, with basal half of mid femora and three-fifths of hind femora yellow; tibiae fulvous with apical black spur on mid tibiae; tarsi fulvous to yellow.

Wings ( Fig. 7A ). Length 6.78-7.78 mm; basal costal (bc) and costal (c) cells fuscous; microtrichia in outer corner of cell costal only; remainder of wings with a pale fulvous tint except fuscous subcostal cell, broad fuscous costal band overlapping confluent with R4+5, a large dark fuscous apical spot from apex of R2+3, and englobing apical portions of veins R4+5 and M (from interception with dm-cu), a broad fuscous anal streak ending at apex of A1 + CuA2; dense aggregation of microtrichia around A1 + CuA2; supernumerary lobe well developed.

Abdomen ( Figs 6C, D , 7C ). Elongate oval and petiolate; terga free; pecten present on tergum III; posterior lobe of surstylus long ( Fig. 7C ); abdominal sternum V with a shallow concavity on posterior margin. Tergum I as long as wide and sterna I and II longer than wide. Tergum I fulvous with apical margin narrowly yellow. Tergum II yellow with a median fulvous heart-shaped marking with an inner anteromedial fuscous marking. Terga III–V fulvous with fuscous along basal margin of tergum III, a narrow median longitudinal band reaching apex of tergum V, narrowly along lateral margins of terga III–IV, and as broad lateral markings on tergum V, anterior to ceromata, and with pale fulvous along base of tergum IV and on tergum V medially, anterior to ceromata (shining spots), which are also pale fulvous.

Etymology.

The species name is an adjective that refers to the Madhupur National Park, where all specimens were collected.

Figures

Figure 5. Maximum likelihood tree of COI- 3 P sequences of Zeugodacus madhupuri sp. nov. and its closest relatives in COI. Taxa names include UHIM ' ms' molecular voucher numbers, GenBank accessions and ISO three letter country codes. The record of Zeugodacus hengsawadae was published as Zeugodacus nr. tau in San Jose et al. (2018 a). The scale bar indicates substitutions per site; values on the branches indicate ultrafast bootstrap support values and Sh-aLRT bootstrap values, respectively.

Figure 6. Zeugodacus madhupuri sp. nov. A head B head and scutum C-D abdomen.

Figure 7. Zeugodacus madhupuri sp. nov. A wing B lateral view C terminal abdominal segment.