Dolichocephala monae Joost, 1981

Kazerani, F., Shamshev, I. V., Morinière, J. & Thorn, S., 2022, New records of Empididae and Hybotidae (Insecta: Diptera) from Hyrcanian forest in Iran and Azerbaijan, Far Eastern Entomologist 462, pp. 20-28 : 22-24

Dolichocephala monae Joost, 1981

Figs 2–5

MATERIAL EXAMINED. Iran: Golestan province [Shast-Kola forests], 36°43'10'' N ,

54°24' 17'' E, 1320 m, sweeping net, 10.VI 2017, 3♂, leg. F. Kazerani [ ACFRI]; [Shast-Kola forests] , 36°43'00.7" N 54°23'13.7" E, 1271 m, pan traps, 12.VII 2017, 6♀, leg. F. Kazerani

[ ACFRI]; Gilan province [Shafarood forests], 37°40'15.1"N, 48°45'09.6" E, 1138 m, pan traps ,

15.VII 2018, 1♀, leg. F. Kazerani [ ACFRI]. Azerbaijan : Lankaran [Khanbulan], 38°40' N

48°48' E, 20.V 2008, 1♂, leg. N. Vikhrev [ ZMUM]; Hirkan-Burjeli env ., 38°39´N 48°47´E,

15–22. V 2009 , 1♀, leg. K. Tomkovich [ ZMUM]; Hirkan-Burjeli env., 28. V 2009, 1♂, leg.

K. Tomkovich [ ZMUM]; Hirkan-Burjeli env., 15–18. V 2009, 6♂, 4♀, leg. I. Grichanov & K.

Tomkovich [ ZISP].

DIAGNOSIS. This is a dark species distinguished by extensively yellowish legs, brown halters and eleven round white wing spots. Male terminalia: hypandrium cone-shaped, with several short setae; phallus slender and sclerotized basally; epandrium rounded; clasping cercus deeply divided into two subequal in length processes; surstylus slender, tapered, apex pointed ( Figs 2–5 ).

REMARKS. First record for Iran. Up to now, Dolichocephala monae was known after the type specimens only from Armenia (Joost, 1981; Sinclair & Shamshev, 2014) .

In the collected specimens of D. monae from Hyrcanian forests, we faced a different pattern on the wing so that it has eleven spots and this is a fixed character in all collected specimens from Iran and Azerbaijan while in the original description mentioned wing with seven white rounded spots. However, the structures of the male terminalia, including the shape of the clasping cercus and the phallus, are quite identical to the holotype ( R. Wagner,

pers. comm.). The wing pattern of D. monae is variable in specimens collected from Georgia

(B.J. Sinclair, pers. comm.). Therefore, we considered that the above-mentioned specimens belong to D. monae .

female, lateral view; 4 – male head, lateral view; 5 – wing.

In Europe, based on the species collected in emergence traps placed near water and wet areas, it is supposed that larva of the genus Dolichocephala are aquatic (Wagner & Gathmann, 1996; Meyer & Filipinski 1998; Sinclair & MacDonald, 2012; Wolton et al., 2014).

DISTRIBUTION. Palaearctic: Armenia, Azerbaijan, Georgia, Iran.

MOLECULAR DELINEATION WITHIN DOLICHOCEPHALA . The barcodes obtained during this study were used to calculate the evolutionary distances, shown on a neighbourjoining tree ( Fig. 6 ) which comprises seven well-separated clusters, representing nine species of Dolichocephala . Comparison of the CO1 DNA barcode data, as well as p-distances to conspecifics, revealed a mean interspecific distance of 7.9% between D. monae and D.

thomasi ( Fig. 6 ). The latter species has wing with ten white rounded spots and clearly different structure of the male terminalia (Wagner, 1983). The COI barcode data showed slight difference between D. guttata and D. oblongoguttata . These species are distingui-

shable only by details of the male terminalia (Drake & Chandler, 1997).

genus Dolichocephala Macquart created in BOLD. Corresponding BIN (Barcode Index

Numbers – www.boldsystems.org) are highlighted within species names.

Subfamily Empidinae Schiner, 1862

Figures

Figs 2–5. Habitus and wing images of Dolichocephala monae. 2 – male, lateral view; 3 –

Fig. 6. Neighbor-joining tree based on genetic divergences on the CO1 gene within the