Mucispora hydei Wijayaw., Q.R. Li, Y.C. Deng, L.S. Dissan & D-Q Dai

Wijayawardene, Nalin N., Dissanayake, Lakmali S., Li, Qi-Rui, Dai, Dong-Qi, Xiao, Yuanpin, Wen, Ting-Chi, Karunarathna, Samantha C., Wu, Hai-Xia, Zhang, Huang, Tibpromma, Saowaluck, Kang, Ji-Chuan, Wang, Yong, Shen, Xiang- Chun, Tang, Li-Zhou, Deng, Chun-Ying, Liu, Yanxia & Kang, Yingqian, 2021, Yunnan-Guizhou Plateau: a mycological hotspot, Phytotaxa 523 (1), pp. 1-31 : 4-5

Mucispora hydei Wijayaw., Q.R. Li, Y.C. Deng, L.S. Dissan & D-Q Dai sp. nov.

( FIGURE 1 )

Index Fungorum number: IF558463

Etymology:— Named in honour of British mycologist, K.D. Hyde for his immense contributions to mycology

Holotype:— GMB0028

Saprobic on decaying wood. Asexual morph Hyphomycetous. Conidiophores 60–110 × 8–12 µm (x̅ = 78.6 × 9.8 µm, n = 30), macronematous, mononematous, erect, solitary or in small groups on compactly aggregated cells, simple, cylindrical, smooth, brown, straight or slightly flexuous, percurrently proliferate 2–3 times, 1–2-septate. Conidiogenous cells holoblastic, integrated, terminal, cylindrical, smooth, pale brown. Conidia 35-50 × 20-30 µm (x̅ = 41.2 × 25.5 µm, n = 30), acrogenous, solitary, simple, smooth, ellipsoidal to obovoid, hyaline to subhyaline when young, dark brown when mature, with obvious septa in young conidia, paler at basal cell, truncate at base, sometimes covered by a hyaline mucilaginous sheath. Sexual morph Undetermined.

Material examined:— CHINA, Guizhou Province, Guiyang, Gaopo Township, Raorao village (106°48’6.54”E, 26°19’3.46”N), on decaying submerged wood, 9 th December 2019, Nalin N. Wijayawardene, Q. R Li, ( GMB0028 , holotype, NNW56 , isotype) .

LSU: MW797122 , SSU MW 800164 , ITS MW 797039 (Supplementary Table 1)

Known distribution:— Guizhou Province, China

Notes:— Yang et al. (2016) introduced the genus Mucispora Jing Yang et al. with M. obscuriseptata J. Yang et al. as the type species. Besides the type species, the genus comprises two species viz. M. phangngaensis J. Yang & K.D. Hyde ( Yang et al. 2017) and M. infundibulata J. Yang & K.D. Hyde ( Hyde et al. 2020) . All these species have been reported from submerged plant materials in Southern Thailand (Prachuap Khiri Khan Province and Phang Nga Province). In morphology, Mucispora closely resembles Melanocephala but it is specific in its cupulate proliferating conidiogenous cells and its conidia bearing a central downwardly directed collar with a fimbriate margin’ ( Hughes 1979; Yang et al. 2017).

Our new collection did not germinate in different media (WA, PDA, MEA) and in different temperatures thus we extracted DNA directly from the fruiting body ( Zeng et al. 2018). PCR amplification of ITS (ITS4/ ITS5), LSU (primers: LR5/ LROR) and SSU (primers: NS1/NS4) were successful.

Phylogenetic analyses of combined LSU and ITS genes ( Fig. 2 ) that our new strain is distinct from other taxa. However, the separation value is medium (69% in ML) and PP value is low. Nevertheless, morphological characters, of our collection is well-distinct from other Mucispora species ( Table 2 ). Hence, we introduce the fourth species of the genus, Mucispora hydei . This is the first record of the genus outside Thailand.

Figures

FIGURE. Mucispora hydei (GMB0028, holotype). a. Decaying wood. b, c. Colony on wood. d, e, g. Conidiophores with conidia. f. Matured conidia. h–j. Conidiophore. Scale bars: b =100 µm, c =200 µm, d, e, h–j = 20 µm, f, g = 40 µm.

FIGURE. RAxML tree based on a combined dataset of partial LSU and ITS sequence analyses. Bootstrap support values for ML equal to or greater than 60 %, Bayesian posterior probabilities (BYPP) equal to or greater than 0.95 are shown as ML/ BYPP above the nodes. New isolates are in red bold. The tree is rooted to Conioscypha lignicola and Conioschypha minutispora (FMR11245) and Conioscyphascus varius. The scale bar represents the expected number of nucleotide substitutions per site.