Miltogramma rognesi Szpila and Pape, 2024

Szpila, Krzysztof, Walczak, Kinga, Grzywacz, Andrzej, Akbarzadeh, Agnieszka Soszyńska Kamran, Bystrowski, Cezary & Pape, Thomas, 2024, Underexplored diversity in Palaearctic Miltogramma Meigen, 1803 (Diptera: Sarcophagidae), Zoological Journal of the Linnean Society 201, pp. 1-21 : 9

Miltogramma rognesi Szpila and Pape , sp. nov.

( Figs 1E , 3E , 6A–M )

Zoobank registration: urn:lsid:zoobank.org:act:4AB79A4D-C01F-41A8-8857-E3E4D19F46CD .

Type material. Holotype: 1M, Iran, North Khorasan, Lowjalli, 15 May 2014, 37.6749 57.8548, leg. KEiB Iran Expedition . Paratypes: 1M, Iran, Kerman, Bolbolooine , 17 April 2015, 30.1517 57.4048, leg. KEiB Iran Expedition II; 1M,1F , Iran, Kerman, Sirch road, 19 April 2015, 30.1957 57.4357, leg. KEiB Iran Expedition II; 1M , Iran, Tehran, Emamzadeh Hashem , 27 June 2016, 35.7863 52.0346, leg. KEiB Iran Expedition III; 1M , Iran, Kerman, Jupar Massive , 10 May 2017, 29.9230 57.2703, leg. KEiB Iran Expedition IV; 1M , Iran, Kerman, Rayen Waterfall , 17 May 2017, 29.5438 57.2981, leg. KEiB Iran Expedition IV .

Male ( Figs. 1E , 3E , 6A–J ). Body length 6–8 mm (N = 7). Head. Parafacial plate pale whitish, broad, slightly widened posteriorly, with the same width at level of vibrissal angles and at the base of antenna. Frons broad, almost parallel, 0.35 head width at the ocellar triangle and 0.32 head width at the base of antenna. Fronto-orbital plate white-silver including posteriormost part. Frontal viưa broad, 2.5× as broad as fronto-orbital plate at vertex, widened posteriorly, width at lunule 0.7 width at anterior ocellus, shining-silver in anterior view, brown in dorsal view. Scape, pedicel, first flagellomere, and arista black. Ocellar and postocellar setae not differentiated from numerous long, hair-like, additional ocellar setulae. Inner vertical seta strong, curved, outer vertical seta also curved and 0.7× as long as inner vertical seta; 14–16 pairs of long frontal setae, setae crossed over the frontal viưa. Frontal viưa and vertex bare, one pair of paravertical setae present. Four to five proclinate orbital setae and one reclinate orbital seta, both strong. Upper part of fronto-orbital plate with numerous long setulae distributed around orbital setae. Parafacial plate with sparse, long, black setulae. Lunule bare. Scape and pedicel with short setulae. Gena and postgena with long, black setulae. Postcranium with black setulae. Antenna inserted distinctly above level of middle of eye. Antenna short, first flagellomere 1.8× as long as pedicel and with the same length as distance between tip of first flagellomere and lower margin of facial plate. Arista micropubescent, aristomere 1 shorter than their greatest diameter and aristomere 2 as long as their greatest diameter, aristomere 3 widened in basal 0.6. Facial plate with low keel below antennal insertion. Supravibrissal and subvibrissal seta numerous, long. Vibrissa not differentiated. Vibrissal angles situated slightly above lower margin of facial plate. Height of gena 0.6× length of first flagellomere and 0.16× eye height. Proboscis long, labellum 0.5× as long as prementum, palpus yellow.

Thorax. Black ground colour; covered with sparse grey microtrichosity; scutum with unclear median stripes, presutural area with five stripes: a pair of broad lateral stripes flank three narrow inner stripes; postsutural area with three stripes: the continuation of the broad lateral stripes and the narrow median stripe. Entire surface of the thorax with dense and long setulae.

Legs. Foreleg tarsomeres 1–5 with length proportions 1–0.4– 0.4–0.5–0.8. Fore tarsus with claws shorter than tarsomere 5. Tarsomeres 2–4 with elongated dorsal and posterodorsal setae. All setae very long, setae straight, curved only distally. Mid-tibia with two strong and one to three weak anterodorsal setae. Legs otherwise without particular modifications.

Wing. Tegula brown, basicosta yellow, veins yellow; costal spine not developed; base of vein R 4 * 5 with 1–3 setulae; distal part of vein M bent at right angle, M cell r 4 * 5 open.

Abdomen.Generally black, covered with grey microtrichosity. Colour paưern on tergites 3–5 variable with direction of observation; all tergites with a black band across posterior margin in dorsal and posterodorsal views. This black band is interrupted dorsally by a narrow strip of grey microtrichosity. In lateral view, tergites 3–5 with unclear border between the band and surface with microtrichosity.

Terminalia ( Fig. 6H–J ). Cuticule black, with sparse grey microtrichosity. Cercus (c) black, curved (lateral view) and gradually tapering into a pointed tip (lateral and posterior view). Cercus with scaưered setae and short setulae in basal half, almost bare in distal half. Gonostylus (gst) black, straight, shorter than cercus, evenly rounded apically and with setulae along whole length. Pregonite (prg) very broad, slightly curved anteriorly with rounded tip; postgonite (pog) almost straight, broad with rounded tip and strong anterior seta. Basiphallus (bp), ventral plate (vp) and dorsal plate (dp) strongly sclerotized; dorsal plate shallowly bifid apically (posterior view); ventral plate much shorter than dorsal plate. Membranous part of phallus broad, with numerous denticles, acrophallus (ac) widened at tip.

Female ( Fig. 6K–M ). Differs from male by absence of silver microtrichosity on the frons and unmodified fore tarsus chaetotaxy.

Biology. Specimens were collected around noon, perching on stones along dry stream beds and on gravel roads, in mountainous habitats (more than 2000 m a.s.l.).

Barcoding. Barcoded specimen (male, GenBank accession KY749737) was grouped as sister to species Miltogramma vera ( Verves, 1979) ( Table 2 ; Fig. 4 ).

Etymology. Named in honour of recently deceased, eminent Norwegian dipterist, Prof. Knut Rognes.

Remarks. Identifying specimens of M. rognesi sp. nov. using the keys provided by Rohdendorf (1930, 1935) and Verves (1994) leads to species belonging to subgenus Rohdendorfiella Verves and further to Miltogramma (Rohdendorfiella) vera . The fore tarsus chaetotaxy with absence of elongated dorsal and posterodorsal setulae on the fissh tarsomere ( Figs 6G , 7E, F , 8E ) and difference in barcode sequence separates M. rognesi from the M. vera ( Fig. 4 ). DNA sequences in Piwczyński et al. (2017) identified as ‘ Miltogramma vera ( Verves, 1979) ’ refer to M. rognesi sp. nov

Figures

Figure 1. Male fore tarsus of Miltogramma spp. A, M.vervesi sp. nov., right tarsus, tarsomeres 2–5, anterior view. B, M.mikolajczyki sp. nov., right tarsus, tarsomeres 4 and 5, dorsal view.C, M.manouchehrii sp. nov., less tarsus, tarsomeres 4 and 5, dorsal view.D, M.soszynskii sp.nov., right tarsus, tarsomeres 1–5, anterior lateral view.E, M. rognesi sp. nov., right tarsus, tarsomeres 1–5, anterior lateral view.

Figure 3. Male fore tarsus of Miltogramma spp.A, M.vervesi sp. nov., right tarsus, anterior lateral view. B, M.mikolajczyki sp. nov., right tarsus, dorsal view. C, M. soszynskii sp. nov., right tarsus, anterior lateral view. D, M.thompsoni sp. nov., right tarsus, anterior lateral view. E, M.rognesi sp.nov., right tarsus, anterior lateral view.F, M.vera, right tarsus, anterior lateral view. G, M. manouchehrii sp. nov., right tarsus, dorsal view. Scale bars:0.2 mm.

Figure 4. Neighbour-joining phylogenetic tree based on COI gene sequences of Miltogramma species.Numbers at branch nodes represent bootstrap support values as a percentage of 1000 replicates. The scale bar, measured in 0.2 substitutions per nucleotide position, indicates the genetic distance between the species. The asterisk (*) in Miltogramma efflatouni indicates a specimen that was misidentified as Miltogramma nuda in Kuưy et al. (2010).

Figure 6. Miltogramma rognesi sp. nov.(male paratype:Iran, Kerman, Sirch road, 19 April 2015, 30.1957 57.4357, leg. KEiB Iran Expedition II, NHMD; female paratype: Iran, Kerman, Sirch road, 19 April 2015, 30.1957 57.4357, leg. KEiB Iran Expedition II, NHMD; male genital apparatus: paratype Iran, Kerman, Bolbolooine, 17 April 2015, 30.1517 57.4048, leg. KEiB Iran Expedition II, NHMD). A, male, habitus, lateral view.B, male, habitus, dorsal view.C, male, abdomen dorsal view.D, male, head, lateral view.E, male, head, frontal view. F, male, head, dorsal view. G, male, right fore tarsus, anterior view.H, epandrial complex, dorsal view. I, epandrial complex, lateral, view. J, phallus, pre- and post gonites, ejaculatory sclerite, hypandrium.K, female, head, lateral view. L, female, head, frontal view.M, female, head, dorsal view.

Figure 7. Miltogramma soszonskii sp. nov. (male paratype: Iran, Kerman, Hinaman I, 9 May 2017, 30.5426 57.2818, leg. KEiB Iran Expedition IV, KEIB_DIP_01221, NHMD; male genital apparatus:paratype Iran, Kerman, Hinaman II, 16 May 2017, 30.5654 57.2952, leg. KEiB Iran Expedition IV, NHMD). A, habitus, lateral view. B, habitus, dorsal view.C, abdomen, dorsal view.D, head, lateral view. E, head, frontal view. F, head, dorsal view.G, right fore tarsus, anterior lateral view.H, wing, dorsal view. I, epandrial complex, dorsal view.J, epandrial complex, lateral, view. K, phallus, pre- and postgonites, ejaculatory sclerite, hypandrium.

Figure 8. Miltogramma thompsoni sp. nov. (male holotype:Uzbekistan, Besh-Bulak, 25 June 2019, 39.3762 63.1735, leg. KEiB Expedition, NHMD; female paratype: Uzbekistan, Besh-Bulak, 25 June 2019, 39.3762 63.1735, leg. KEiB Expedition, NHMD; male genital apparatus: paratype Uzbekistan, Besh-Bulak, 25 June 2019, 39.3762 63.1735, leg.KEiB Expedition). A, male, habitus, lateral view.B, male, habitus, dorsal view. C, male, head, lateral view.D, male, head, frontal view. E, male, head, dorsal view.F, male, wing, dorsal view. G, male, right fore tarsus, anterior lateral view. H, epandrial complex, dorsal view. I, epandrial complex, lateral, view. J, phallus, pre- and postgonites, ejaculatory sclerite, hypandrium. K, female, head, lateral view. L, female, head, frontal view. M, female, head, dorsal view.