Pusulissus phiaoacensis, Bourgoin & Wang, 2020

Bourgoin, Thierry & Wang, Menglin, 2020, Parahiraciini (Hemiptera, Fulgoromorpha, Issidae): one new genus, two new species and three new subtribes, ZooKeys 997, pp. 69-94 : 69

Pusulissus phiaoacensis sp. nov. Figs 2-7 , 8-12 , 13-14 , 15-20 , 21-24

Type materials.

Holotype: ♂, VIetnam: Phia Oac, 12 vii 2019, 1050 m, (22°26'0.78"N, 105°53'4.692"E), CAV [chasse à vue], rec. Th. Bourgoin, MNHN(EH) 24743. Paratypes: 1♀, same data as holotype, MNHN(EH) 24744 [sequenced specimen]; 1♀, same location and collection date as holotype, but rec. G. Kunz MNHN(EH) 24745.

Description.

Length: male (including forewings) (N = 1): 5.0 mm; female (including forewings) (N = 2): 5.1-5.3 mm.

Coloration. For the dry specimens, general coloration tawny (Fig. 2 ). Vertex tawny, with two brown circular markings near base of disc (Fig. 4 ). Compound eyes black, supported by tawny callus (Figs 4 , 5 ). Frons brown, dorsal part black (Fig. 5 ); dorsal and lateral margins carinated and brown, median carina brown (Fig. 5 ). Frons with two tawny round markings in middle area, dorsal and lateral areas distributed with around ten tawny tubercles on each side (Fig. 5 ). Antennae brown (Fig. 5 ). Postclypeus brown (Fig. 5 ). Genae tawny (Fig. 3 ). Pronotum tawny, median area with two small brownish impressions on disc (Fig. 4 ), paranotal lobes tawny (Fig. 5 ). Mesonotum tawny, with two brown longitudinal bands on disc (Fig. 4 ). Forewings tawny with veins tawny, basal part of costal area with some irregular black markings, area from basal 1/3 of costal margin extending to middle of forewing surface also with some irregular black markings, apical margin with black linear marking (Fig. 6 ); some specimens with these black markings almost invisible, but with one extremely large whitish round marking at basal 1/3 of forewing on each side (Fig. 2 ). Hindwings brown, veins clearer (Fig. 7 ). Legs tawny (Figs 3 , 5 ). For alive and fresh specimens, vertex, pronotum, mesonotum and forewings interspersed with some reddish markings or coloration as mentioned above (Figs 21-23 ). Compound eyes yellow (Figs 21-23 ). Frons brown, dorsal part black, median carina brown, disc distributed with several yellow tubercles (Fig. 22 ). Legs brown (Figs 21-23 ).

Head and thorax. Vertex 2.1 times wider than long in midline, anterior margin very slightly angularly convex (Fig. 4 ). Frons 1.3 times longer in midline than widest part, 1.3 times wider at widest part than dorsal margin (Fig. 5 ). Pronotum 1.4 times longer in midline than vertex in midline (Fig. 4 ). Mesonotum 2.3 times wider along anterior margin than long in midline (Fig. 4 ). Forewings 2.1 times longer at longest part than widest part (Fig. 6 ). Hindwing with apical margin of CuP-Pcu-A1 lobe sinuate (Fig. 7 ). Metatibiotarsal formula: 2-8/5/2.

Male genitalia. Anal tube in lateral view with lateral lobes well visible (Fig. 8 ); in dorsal view mushroom shaped, widest at middle, as long in midline as widest part, apical margin angularly rounded, lateral margins strongly angularly rounded (Fig. 9 ); anal opening located near middle of anal tube (Fig. 9 ). Pygofer in lateral view 2.5 times higher than wide, dorsal margin obviously sloping to the posterior, dorso-lateral angle rounded, posterior margin strongly roundly convex (Fig. 8 ). Gonostylus in lateral view with dorsal margin slightly sinuate (Fig. 10 ). Capitulum of gonostylus derived from middle of gonostylus, broad, sharp triangular, directed to anterior; meniscate processes with one side not reaching to anterior margin of capitulum and another side beyond posterior margin of capitulum (Fig. 10 ). Periandrium in lateral view with dorso-lateral lobe rounded apically (Figs 11 , 13 ). In posterior view, ventral lobe of periandrium with apical margin mostly straight but spinous protruded at middle (Fig. 14 ). Lateral processes of aedeagus long and slender, hooked, derived from the middle, directed anteriorly to basal 1/4, then curved upward, surpassing the dorsal margin of periandrium (Figs 11 , 13 ); in ventral view this pair of processes curved outwards (Fig. 12 ).

Female genitalia. Anal tube in dorsal view broadly ovate, 1.1 times longer in midline than widest part, widest at middle, apical margin nearly straight, lateral margins rounded, anal opening situated slightly below middle (Fig. 15 ). Anterior connective lamina of gonapophysis VIII with two obscure large teeth in the apex and four small keeled teeth in outer-lateral group (Fig. 20 ). Gonocoxa VIII subquadrangular (Fig. 20 ). Hind margin of sternite VII roundly concave, with the median part nearly straight (Fig. 19 ).

Etymology.

The name refers to the locality where the new species was found.

Habitat.

The species was swept from pteridophytes at the margin of the mountainous rainforest (around 1050 m altitude) beside a road (Fig. 24 ).

Note.

Genes sequences were registered in GenBank with the following accession numbers: MW201961 (18S), MT774095 (COXI), MT774092 (Cytb). P. phiaoacensis differs from another unnamed Pusulissus species (referred here as Pusulissus sp.) (Fig. 46 ) by 25 bp in the length 681 bp of COXI. Both species are from Vietnam.

Figures

Figure 46. Bayesian 50 % consensus tree of Parahiraciini species based on combined sequences (18 S, 28 S, COXI, Cytb) with 2 species of Sarimini and 2 species of Hemisphaeriini as outgroup. At each node, values denote posterior probability support.

Figures 2 - 7. Pusulissus phiaoacensis sp. nov. 2 adult (holotype), dorsal view 3 adult (holotype), lateral view 4 head and thorax (holotype), dorsal view 5 adult (holotype), frontal view 6 forewing (paratype) 7 hindwing (paratype). Scale bars: 1 mm.

Figures 8 - 12. Pusulissus phiaoacensis sp. nov., male, holotype. 8 genitalia, lateral view 9 anal tube, dorsal view 10 gonostylus, lateral view 11 phallic complex, right lateral view 12 phallic complex, ventral view. Scale bars: 0.2 mm.

Figures 13 - 14. Pusulissus phiaoacensis sp. nov., male, holotype. 13 phallic complex, right lateral view 14 apex of phallic complex, posterior view. Abbreviations: dll: dorso-lateral lobe of periandrium; Ade: Aedeagus; vl: ventral lobe of periandrium; Adep: Aedeagus processes. Scale bars: 0.2 mm.

Figures 15 - 20. Pusulissus phiaoacensis sp. nov., female, paratype. 15 anal tube, dorsal view 16 gonoplac, lateral view 17 gonapophysis IX and gonospiculum bridge, dorsal view 18 gonapophysis IX and gonospiculum bridge, lateral view 19 sternite VII, ventral view 20 gonocoxa VIII and gonapophysis VIII, lateral view. Scale bars: 0.2 mm.

Figures 21 - 24. Habitus and habitat of Pusulissus phiaoacensis sp. nov. 21 - 23 habitus in nature (Photo G. Kunz) 24 habitat (Photo T. Bourgoin).