Caudiurostyla sinensis Lyu et al., 2018

Min, Kyu-Seok Chae and Gi-Sik, 2023, New record of three hypotrich soil ciliates (Ciliophora: Hypotricha) from South Korea: Oxytricha multilineata, Mixophrya pantanalensis pantanalensis and Caudiurostyla sinensis, Journal of Species Research 12 (4), pp. 307-312 : 310-311

Caudiurostyla sinensis Lyu et al., 2018 ( Fig. 3 )

ŔḤAEŔŤÜAEệ (ljḡ)

Caudiurostyla sinensis Lyu et al., 2018: p. 168 - 170, Figs. 1 , 2 , Table 1.

Material examined. Soil sample from Miho River, Cheongju­si, Chungcheongbuk­do, Korea (36°38 ʹ 3.54 ʺ

N, 127°21 ʹ 18.44 ʺ E), collected by Kyu­Seok Chae on 15 September 2020.

Diagnosis. Cell size 143 - 202 × 40 - 58 μm in protargol impregnation; body flexible with slender to elliptical shape; 46 - 60 adoral membranelles; 1 × 3 - 4 μm colorless cortical granules present; 100 - 177 macronuclear nodules with 3 - 6 micronuclei; 11 - 15 frontal cirri; 2 frontoterminal cirri; 11 - 15 midventral pair; 6 - 7 left and 5 - 7 right marginal row; 5 or 7 transverse cirri; 4 or 5 dorsal kineties; 3 - 5 caudal cirri.

Distribution. China and South Korea.

Remarks. The Korean population of C. sinensis is consistent with the type population (Chinese population) in terms of ventral and dorsal ciliature and the number of macronuclear nodules ( Lyu et al., 2018). Caudiurostyla sinensis resembles Hemicycliostyla franzi (Foissner, 1987) Paiva et al., 2012 , but two species can be distinguished by the number of caudal cirri (3 or 5 vs. absent) and buccal cirri (1 vs. 2 - 3) ( Li et al., 2018).

The 18S rDNA sequence of C. sinensis was 3026 bp in length (GenBank accession number: OQ919809). The 18S rDNA gene sequences of the Korean and Chinese populations are 100% identical ( Table 2).

Voucher slides. One slide with protargol­impregnated specimens was deposited at the National Institute of Biological Resources (NIBRPR0000111060).

Figures

Fig. 3. Caudiurostyla sinensis from life (A-E) and after protargol impregnation (F, G). A, B, Ventral view showing contractile vacuole (arrow); C, Ventral view from live; D, Ventral view showing cortical granules (arrows) lying along with cirral; E, Side view of cortical granules(arrow); F, G, Ventral and dorsal view of typical specimen; FC, frontal cirri; LMR, left marginal cirral row; RMR, right marginal cirral row; TC, transverse cirri. Scale bars=10 μm (D, E); 50 μm (A, B, C, F, G).

Fig. 1. Oxytricha multilineata from life (A, B) and after protargol impregnation (C, D). A, Ventral view showing cytoplasm and arrow indicating contractile vacuole; B, Dorsal view showing dorsal bristles (arrows); C, D, Ventral and dorsal view of typical specimen; AZM, adoral zone of membranelles; CC, caudal cirri; DM, dorsomarginal row; FC, frontal cirri; LMR, left marginal cirral row; PTC, pretransverse cirri; RMR, right marginal cirral row; TC, transverse cirri; 1-5, dorsal kineties 1-5. Scale bars=50μm.

Fig. 2. Mixophrya pantanalensis pantanalensis from life (A-C) and after protargol impregnation (D, E). A, Ventral view showing lithosomes (arrows); B, Cytoplasm showing cortical granule (arrows) and mitochondria (arrowheads); C, Cytoplasm showing surrounded lithosome (arrow) from crystalline structures and masses of granules (arrowhead); D, E, Ventral and dorsal view of typical specimen; AZM, adoral zone of membranelles; BC, buccal cirrus; CC, caudal cirri; FC, frontal cirri; LMR, left marginal cirral row; PTC, pretransverse cirri; RMR, right marginal cirral row; TC, transverse cirri; 1-6, dorsal kineties 1-6. Scale bars=10μm (B, C); 50μm (A, D, E).