Polymastia norfanzii, Ekins & Erpenbeck & Wörheide & Hooper, 2023

Ekins, Merrick, Erpenbeck, Dirk, Wörheide, Gert & Hooper, John N. A., 2023, Deep Water Polymastiidae (Porifera, Polymastiida) from the South West Pacific, Zootaxa 5369 (1), pp. 57-88 : 78-82

Polymastia norfanzii sp. nov. Ekins, Erpenbeck & Hooper urn:lsid:zoobank.org:act:6B12CAAA-B341-4860-9E45-F33DBB995B5D

Figures 1 , 2 & 10 , Table 5

Material examined: Holotype QM G339367 , Lord Howe Plateau , Pacific Ocean Seamounts, Australia -34.2315, 162.6765, 515–700 m, Sherman Sled, Coll. NORFANZ expedition on RV Tangaroa , 85-014, MF336, 26/V/2003.

Etymology: named in honour of the NORFANZ expeditions which enabled the collection of these new species.

Diagnosis: Polymastia with a single invaginated non-contractile papilla, choanosomal tracts of large tylostyles, forming bouquets below the ectosome. Smaller tylostyles also in the ectosome and scattered in the choanosome, lacking stellate bundles of tylostyles in the choanosome.

Morphology: The sponge is dome shaped 11 mm in diameter and 5 mm in height. It was originally attached to a rock. It is cream in colour and has a hispid surface ( Fig. 10 A ). The upper surface bears a single non-contractile papilla 2 mm in length and approximately 0.5 mm in width, which is invaginated into the upper surface ( Fig. 10 B, C ). There is a single oscule on the summit of the papilla of 0.1 mm in diameter ( Fig. 10 A–C ).

TABLE 5. (Continued)

Skeleton: Aquiferous canals are obvious throughout the choanosome and continue up through the papillae ( Fig 10 B, C ). The choanosomal skeleton is composed of longitudinal tracts of principal styles, with smaller tylostyles scattered between the tracts ( Fig. 10 C, D ). These tracts originate in the base of the sponge and form bouquets beneath the ectosome and then protrude through the ectosome where they form the outer hispid layer. The walls and exterior face of the single papilla are protected and supported by the tylostyles ( Fig. 10 C ). The ectosomal skeleton is composed of the tightly packed bouquets of ascending styles forming a dense palisade along with the smaller tylostyles ( Fig. 10 E ).

Spicules. Principal styles 499–(1226)–2560 × 6.1–(17.4)–30.2 μm, (n=62) ( Fig. 10 F,G ), small tylostyles 92.7– (140)–272 × 2.9–(4.5)–8.7 μm, (n=52) ( Fig. 10 H ).

Molecular data: 28S-C region barcode of holotype QM G339367 (ENA Accession number OY741341), This sample is genetically different from all other samples analysed in this study. It forms the sister to a supported P. invaginata clade .

Remarks: This new species is different from P. invaginata firstly by the absence of the stellate bundles of the small tylostyles, that have been consistently referred to as occurring throughout the choanosome. This new species has scattered loose tylostyles in the choanosome and only at the termination of the aquiferous channels do there appear to be tylostyles formed into stellate clusters acting as filters ( Fig. 10 I ). Polymastia norfanzii sp. nov. is also differentiated from P. invaginata by the presence of strictly tylostyles in the choanosomal bundles, rather than the dominating larger styles of the central papillae, surrounded by the invagination caused by partial retraction of the papilla, lacks the contractile ability of the papillae in P. invaginata . This is corroborated by the large number of spicules both internally providing support for the papillae, and externally providing protection ( Fig. 10 C ). The contractile and retractile papilla on P. invaginata have a smooth exterior surface devoid of spicules.

The differences in molecular results obtained in this study compared to previous studies along with the morphological differences mentioned above, separate this new species from P. invaginata . It also raises the possibility that several species may be currently under P. invaginata , as more specimens are recovered in the future, this will most likely elucidate more species.

Figures

FIGURE 1. Distribution of the specimens of species described herein as they were collected during the expeditions.

FIGURE 2. Maximum likelihood phylogenetic tree of polymastiid species reconstructed from 28S rDNA (C-region). Values> 70 of the bootstrap support for the clades are indicated above the branches. Below branches in italics Bayesian inference posterior probabilities ≥ 0.95. The species sequenced in this studied are highlighted in boldface. Scalebar depicts substitutions /site.

FIGURE 10. Polymastia norfanzii sp. nov. A. Plan view of the halved sponge QM G339367 showing the hispid surface, with the single papilla in the upper centre, indicated by the arrow. B. Side view of the same sponge illustrating the aquiferous canals and papilla (indicated by the arrow) as well as the obvious ectosomal palisade. C. SEM of the same sponge illustrating the longitudinal tracts of the diverging bouquets of tylostyles and the protective tylostyles on the outside of the papilla. D. Section illustrating the tracts of choanosomal tylostyles. E. SEM of the ectosome showing the small tylostyles amongst the much larger choanosomal tylostyles. F. Choanosomal tylostyles. G. Magnified ends of the tylostyles in F. H. Small tylostyles. I. Terminating ends of the aquiferous channels.