Ophiostoma tingens (Lagerb. & Melin) Z.W. de Beer & M.J. Wingf., Svensk Skogsvardsfoerening Tidskr. 25:233. 1927.

Min Wang, Hui, Wang, Zheng, Liu, Fu, Xu Wu, Cheng, Fang Zhang, Su, Kong, Xiang Bo, Decock, Cony, Lu, Quan & Zhang, Zhen, 2019, Differential patterns of ophiostomatoid fungal communities associated with three sympatric Tomicus species infesting pines in south-western China, with a description of four new species, MycoKeys 50, pp. 93-133 : 111-112

Ophiostoma tingens (Lagerb. & Melin) Z.W. de Beer & M.J. Wingf., Svensk Skogsvardsfoerening Tidskr. 25:233. 1927. Fig. 14

Description.

Sexual form: unknown.

Asexual forms: Pesotum -like and Sporothrix -like. Pesotum -like: conidiophores macronematous, synnematous; synnemata simple, anchored into the substrate by brown rhizoid-like hyphae, (333-) 344-584 (-684) μm long including conidiogenous apparatus, the base dark brown, slightly widened, (16.7-) 17-50.5 (-65.5) μm wide, the apex cream-coloured or pale brown, slightly widening; conidia hyaline, globose to elliptical, 1-celled, smooth, (2.7-) 3.6-7.2 (-8.0) × (2.8-) 4.3-6.1 (-7.0) μm.

Sporothrix -like: conidiophores semi-macronematous, mononematous, hyaline, simple or loosely branched, smooth, bearing terminal denticulate conidiogenous cells (8.3-) 15.6-30.0 (-42.5) × (1.1-) 1.7-3.1 (-4.7) μm; conidia hyaline, globose to elliptical, obovoid with pointed bases and rounded apices, 1-celled, smooth, (2.6-) 4.0-6.8 (-8.7) × (2.2-) 3.5-5.5 (-7.4) μm.

Culture characteristics.

Colonies on 2% MEA medium slow growing in the dark, reaching 39 mm in diam. in 8 days at 25 °C, growth rate up to 5 mm/day at the fastest; colony margin anomalous. Hyphae appressed to flocculose, black; reverse hyphae also black. Optimal growth temperature 25 °C, no growth at 5 °C and 30 °C.

Known substrate and hosts.

Galleries of Tomicus yunnanensis and T. minor in Pinus yunnanensis .

Known insect vectors.

Tomicus yunnanensis , T. minor .

Known distribution.

Yunnan Province, China; Europe.

Specimens examined.

CHINA, Yunnan, from Tomicus minor and T. yunnanensis galleries in Pinus yunnanensis , Feb. 2017, Nov. 2016, HM Wang, CFCC 52611 = CXY 1866, CFCC 52612 = CXY 1865, CFCC 52613 = CXY 1868.

Note.

Our strains of O. tingens were identified based on phylogenetic affinities and morphological features. (cf. above under note for O. aggregatum .)

Ophiostoma tingens has been reported from sapwood of various Pinus spp. (including P. sylvestris ) infested by T. minor , T. piniperda and Ips sexdentatus in Europe ( Francke-Grosmann 1952, Batra 1967, Jankowiak 2008). The species was recorded in Yunnan Province in China in 2017, associated with T. minor infesting P. yunnanensis ( Pan et al. 2017).