Diaporthe tectonendophytica M. Doilom, A. J. Dissanayake & K.D. Hyde, Fung. Div., 82: 163, 2016

Sun, Wenxiu, Huang, Shengting, Xia, Jiwen, Zhang, Xiuguo & Li, Zhuang, 2021, Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species, MycoKeys 77, pp. 65-95 : 65

Diaporthe tectonendophytica M. Doilom, A. J. Dissanayake & K.D. Hyde, Fung. Div., 82: 163, 2016 Figure 11

Description.

Asexual morph: Conidiomata pycnidial, aggregated, brownish to black, erumpent, subglobose, exuding white creamy conidial droplets from central ostioles after being kept for 30 days in light at 25 °C. Conidiophores 17.4-35.0 × 2.2-3.5 μm, hyaline, branched, subcylindrical, septate, straight or slightly curved, guttulate. Conidiogenous cells 11.3-15.0 × 1.7-2.5 μm (mean = 12.3 × 2.1 μm, n = 20), cylindric-clavate, hyaline, straight to slightly sinuous, tapering towards the apex. Beta conidia 25.0-31.8 × 0.9-1.8 μm (mean = 28.2 × 1.2 μm, n = 20), filiform, hyaline, guttulate, aseptate, hooked and mostly curved through 90-180°, swollen in the middle. Alpha conidia and Gamma conidia not observed.

Culture characteristics.

Pure culture was isolated by subbing hyphal tips growing from surface sterilized diseased material. Colonies on PDA cover the Petri dish diameter after incubation for 15 days in dark conditions at 25 °C, aerial mycelium abundant, white to grayish on surface side, pale yellow on reverse with concentric zonation. Pycnidia are formed on 15th day or later.

Specimens examined.

China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 19 April 2019, S.T. Huang. On diseased leaves of Elaeagnus conferta HSAUP194.11, living culture SAUCC194.11; on diseased leaves of Pometia pinnata HSAUP194.63, living culture SAUCC194.63.

Notes.

Diaporthe tectonendophytica was originally described from the asymptomatic branches of Tectona grandis in Thailand ( Doilom et al. 2017). In the present study, two strains (SAUCC194.11 and SAUCC194.63) from symptomatic leaves of Elaeagnus conferta and Pometia pinnata were congruent with D. tectonendophytica based on morphology and DNA sequences data (Fig. 1 ). We therefore describe D. tectonendophytica as a known species for this clade.

Figures

Figure 1. Phylogram of Diaporthe based on combined ITS, TUB, TEF, CAL and HIS genes. The ML and BI bootstrap support values above 50 % and 0.90 BYPP are shown at the first and second position, respectively. Strains marked with " * " are ex-type or ex-epitype. Strains from this study are shown in red. Three branches were shortened to fit the page size - these are indicated by symbol (//) with indication number showing how many times they are shortened.

Figure 11. Diaporthe tectonendophytica (SAUCC 194.11) a leaf of host plant b, c surface (b) and reverse (c) side of colony after incubation for 15 days on PDA d conidiomata on PDA e, f conidiophores and conidiogenous cells g, h beta conidia. Scale bars: 10 μm (e-h).