Amblycorypha peedee Forrest, 2023

Forrest, Timothy G., Scobie, Micaela, Brueckner, Olivia, Bintz, Brittania & Spooner, John D., 2023, Geographic variation in the calling songs and genetics of Bartram's round-winged katydid Amblycorypha bartrami (Tettigoniidae, Phaneropterinae) reveal new species, Journal of Orthoptera Research 32 (2), pp. 153-170 : 153

Amblycorypha peedee Forrest sp. nov.

Figs 5 , 7 -15 , 17

Material examined. -

Holotype: USA • ♂; North Carolina, Richmond Co., Sandhills Gamelands ; 35.0528°N, 79.6035°W; 1 Jul. 2006; T.G. Forrest leg.; Ambar?-M03-2006; DNA (MS-044) NCBI accession SAMN31929325; REC (2006 Tape01 PGM 04); UNCA to be transferred to FSCA.

Allotype: USA • ♀; North Carolina, Richmond Co., Sandhills Gamelands ; 35.06139°N, 79.63982°W; 16 Jul. 2004; T.G. Forrest leg.; Ambar?-F01-2004; DNA (NA); REC (NA); UNCA to be transferred to FSCA.

Paratypes: (8♂, 0 ♀) USA • 1♂; North Carolina, Richmond Co.; 16 Jul. 2004; T.G. Forrest leg.; UNCA to be transferred to FSCA • 6♂; North Carolina: Richmond Co.; 1 Jul. 2006; T.G. Forrest leg.; UNCA to be transferred to FSCA • 1♂; North Carolina, Richmond Co.; 19 Jul. 2007; T.G. Forrest leg.; UNCA to be transferred to FSCA .

Other specimens:-

2♂, 0♀: North Carolina specimens from Walker et al. 2003 deposited in Florida State Collection of Arthropods. USA • 1♂; North Carolina, Moore Co.; T.J. Walker leg.; (doublet song); FSCA • 1♂; North Carolina, Hoke Co; 26 Jul. 1964; T.J. Walker leg.; FSCA.

Size measurements (mm). -

Holotype: PrnL: 5.9, PrnW: 4.45, TegL: 27.8, TegW: 10.0, HwEx: 3.9, FemL: 27.2, TibL: 28.8mm ( Fig. 17 ). Allotype: PrnL: 7.0, PrnW: 46, TegL: 28.0, TegW: 9.6, HwEx: 2.2, FemL: 29.9, TibL: 31.0, OviL: 11.0mm.

Etymology. -

This species is named for its geographic location, with populations north of the Pee Dee River, which isolates it from populations of A. carolina .

Common name. -

Pee Dee round-winged katydid.

Differential diagnosis. -

Amblycorypha peedee is best distinguished from other species in the Amblycorypha rotundifolia complex by calling song. Syllables are almost always produced in doublets with syllable rates within doublets of about 11.6s-1 at 25°C ( Fig. 8 ) and rates of 4.0s-1 at 25°C between doublets. The sustained portion of calling songs of A. peedee has about 17 syllables with 2-3 syllables in each of the series preceding the sustained portion ( Fig. 7 ).

Description. -

Individuals are typically green with characteristics of the Amblycorypha rotundifolia complex of Amblycorypha ( Walker et al. 2003). The male’s calling song consists of a single syllable type that are produced in several (4-12) bouts of a 2-3 syllables preceding a sustained series of about 17 syllables ( Figs 5 , 7 ) that occur in pairs (doublets) ( Fig. 5D ). During the sustained portion of the song, at 25°C the syllable rate between doublets is about 4.0s-1 and is about 11.6s-1 within the doublet. The function of syllable rate within a doublet in response to changes in temperature is rate=0.583(temp)-2.92 ( Fig. 8 ). Female size measurements for the single individual that was collected are PrnL: 7.05; PrnW: 4.58; TegL: 28.0; TegW: 9.63; FemL: 29.9; TibL: 31.0; OviL: 11.0. Males’ measurements (x -̄ ± SE in mm, N) are on average PrnL: 5.84 ± 0.04, 9; PrnW: 4.32 ± 0.06, 9; TegL: 28.2 ± 0.21, 9; TegW: 9.32 ± 0.18, 9; FemL: 27.3 ± 0.38, 6; TibL: 28.7 ± 0.44, 6 (Table 2 ).

Figures

Fig. 5. A-C. Time waveforms (30 s) of calling songs for 3 NC A. bartrami males. Note the variation in the number of syllables that precede the main sustained train of syllables; D. Highlighted portion in C; E. Oscillogram and spectrogram of highlighted portion of D with 6 syllables in 3 doublets. Doublet syllable rates were faster than those of FL A. bartrami and slower than those of GA-AL A. bartrami. FL, GA, AL, and SC males rarely produced doublet syllables.

Fig. 7. Mean (+ / - SE) number of syllables as a function of the temporal relationship between series in male calling song from populations of supposed Amblycorypha bartrami (GA & AL: green, NC: blue, FL: red, SC: orange) and A. parvipennis (black). Triangles (X = 0) represent the mean number of syllables in the sustained main series of songs averaged over the number of males shown in parentheses. Circles are the means for each series preceding the main series with the number of males contributing to the average indicated in parentheses.

Fig. 8. Syllable rate as a function of temperature for populations of Amblycorypha bartrami (GA & AL: green, NC: blue, FL: red, AL: pink, SC: orange) and A. parvipennis (black). Solid symbols are recordings from our research and open symbols are recordings from other published work (Walker et al. 2003 for A. bartrami and A. parvipennis, Shaw et al. 1990 for A. parvipennis).

Fig. 15. Phylogeography of ' A. bartrami '. Phylogenies were determined by a Bayesian multi-locus multi-species coalescent model using a Markov Chain Monte Carlo process. A. Phylogeographic distribution of sampled populations of supposed A. bartrami (AL, FL, GA, NC, and SC) and A. parvipennis; B. Phylogeographic distribution of song morphs that differed in syllable rates as a function of temperature. Plots were constructed using plot. to. map function of the R package phytools (Revell 2012).

Fig. 17. Holotype of Amblycorypha peedee, PeeDee round-winged katydid. A. Dorsal view; B. Lateral view. Scale bars: 5 mm.