Distoseptispora obpyriformis Z.L. Luo & H.Y. Su, Mycosphere 9(3): 452 (2018)

Shen, Hong-Wei, Bao, Dan-Feng, Boonmee, Saranyaphat, Lu, Yong-Zhong, Su, Xi-Jun, Li, Yun-Xia & Luo, Zong-Long, 2024, Diversity of Distoseptispora (Distoseptisporaceae) taxa on submerged decaying wood from the Red River in Yunnan, China, MycoKeys 102, pp. 1-28 : 1

Distoseptispora obpyriformis Z.L. Luo & H.Y. Su, Mycosphere 9(3): 452 (2018)

Fig. 4

Description.

Saprobic on submerged decaying wood in a freshwater stream. Anamorph: Colonies on wood effuse, hairy, dark brown, glistening, solitary or in small group. Mycelium immersed, composed of septate, pale brown to brown hyphae, smooth-walled. Conidiophores (42-)66-103(-115) × 5-6 µm (x̄ = 84 × 6 µm, n = 20), macronematous, mononematous, solitary or in groups, erect, straight or slightly flexuous, cylindrical, unbranched, 4-6-septate, brown, rounded at the apex, slightly enlarged at the base, smooth-walled. Conidiogenous cells (10-)15-22(-25) × 5-6 µm (x̄ = 19 × 5 µm, n = 20), monoblastic, terminal, determinate, subcylindrical, brown, smooth-walled. Conidia (55-)69-126(-168) × 10-12 µm (x̄ = 98 × 11 µm, n = 25), acrogenous, solitary, obclavate, olivaceous to pale or dark brown, truncate at base, tapering towards the apex, straight or slightly curved, constricted at the septa, 7-18-distoseptate, guttulate, thick and smooth-walled. Teleomorph: Undetermined.

Culture characteristics.

Conidia germinating on PDA within 12 hrs and germ tubes produced from apex and septa of conidium. Colonies growing on PDA reaching 4-5 cm in 20 days at 26 °C in the dark, with dense, velvety, middle papillae, pale to dark brown mycelium from above; dark brown from below.

Material examined.

China, Yunnan Province, Dali City, Weishan Yi and Hui Autonomous County, 25°29′31"N, 100°06′56"E, on submerged decaying branches in a freshwater stream, 19 February 2022, Z.Q. Zhang & Q.X. Yang YJ 1-19-1 (HKAS 125823, living culture KUNCC 23-13047) .

Notes.

Phylogenetic analysis revealed that our new strain KUNCC 23-13047 clustered with two strains of Distoseptispora obpyriformis (MFLUCC 17-1694 (ex-type strain) and DLUCC 0867; Fig. 1 ). A comparison of the LSU, tef 1-α and rpb 2 gene of type strains between KUNCC 23-13047 (this study) and MFLUCC 17-1694 (from holotype) revealed 0% (0/1215 bp), 0.37% (3/812 bp, including 1 gaps), 0% (0/838 bp), 0.29% (3/1034 bp), respectively. Although our collection differs significantly in conidia size from the original description of D. obpyriformis ( Luo et al. 2018a), multigene sequence data do not support this collection as a separate species. Similar results have been reported in previous studies and were found in several species in this study ( Yang et al. 2018; Shen et al. 2021; Ma et al. 2022). Therefore, a new additional record of D. obpyriformis is reported from the Red River Basin in Yunnan, China.

Figures

Figure 1. Maximum likelihood (ML) tree is based on combined LSU, ITS, tef 1 - α and rpb 2 sequence data. Bootstrap support values with a ML greater than 65 % and Bayesian posterior probabilities (PP) greater than 0.95 are given above the nodes, shown as " ML / PP ". The tree is rooted to Aquapteridospora fusiformis (MFLUCC 18 - 1606) and A. lignicola (MFLUCC 15 - 0377). New species are indicated in blue and type strains are in bold.

Figure 4. Distoseptispora obpyriformis (HKAS 125823) a, b colonies on woody substrates c conidiophores d, e conidiophores with conidia f conidiogenous cell g-j conidia k germinating conidium l culture on PDA. Scale bars: 30 μm (c-e, g-k); 20 μm (j).